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(a-j): (a) Coccinella septempunctata Linnaeus, 1758; (b) Brumoides suturalis (Fabricius, 1798); (c) Henosepilachna vigintioctopunctata (Fabricius, 1775); (d) Henosepilachna elaterii (Rossi, 1794); (e) Hippodamia variegata (Goeze, 1777); (f) Scymnus (Pullus) quadrillum Motschulsky, 1858; (g) Scymnus (Pullus) posticalis Sicard, 1913; (h) Scymnus (Scymnus) nubilus Mulsant, 1850; (i) Micraspis allardi (Mulsant, 1866); (j) Pharoscymnus flexibilis (Mulsant, 1853).
Source publication
Beetles belonging to family Coccinellidae are predators of various sucking insect pests, some of them are phytophageous. A lot of work for the exploration of these beetles has been done in Pakistan. Layyah being a multi crop area was selected as study area for the exploration of Coccinellidae beetles. A survey was conducted for the collection of Co...
Contexts in source publication
Context 2
... (Pullus) quadrillum found similar with given diagnostic characters description by Ali et al. (2015) and first time reported from Layyah district Scymnus (Pullus) posticalis Sicard, 1913 (Fig. ...
Context 4
... (Scymnus) nubilus found similar with given diagnostic characters description by Ashfaque et al. (2015) and first time reported from Layyah district. Micraspis allardi (Mulsant, 1866) (Fig. ...
Citations
... In maize crops, C. undecimpuncta is reported to be the most abundant during the month of May and the least abundant in June (Mahmoud et al., 2021). Cheilomenus sexmaculata (Fabricius, 1781), was the third most abundant species (10.98%) during both cropping seasons and similar results were also reported from other cereal crops in Punjab, Pakistan (Bodlah et al., 2021). This species feeds on aphids including Rhopalosiphum maidis (Fitch), Aphis gossypii (Glover) and Lipaphis erysimi (Kaltenbach). ...
The Coccinellidae is a highly diversified family of order Coleoptera. Coccinellid ladybirds are well known for their role as biological control agent against varied range of agricultural pests. The samples of coccinellid ladybird collected from Pakistan were identified and characterized as Micraspis allardi (Mulsant, 1866). This is one of the least-studied ladybird species with limited work on its ecological distribution as a biological control agent. The genus Micraspis has vast genetic diversity with a possible presence of unknown number of cryptic species. Sequence information of some species of the genus Micraspis are present in NCBI database. However, least molecular data or sequences describing M . allardi could be available from database. Therefore, morphological and molecular characterization was imperative for this species. Here, the samples collected from sugarcane field of Faisalabad District of Pakistan and were identified by using morphological and molecular protocols. For molecular identification, two different regions of mitochondrial cytochrome c oxidase I (COI) gene (COI-5′ and COI- 3′) were used as molecular markers for the identification of the species. Morphological appearance, DNA sequence similarity searches and phylogenetic analysis collectively indicated it as M . allardi . To the best of our knowledge, this is the first report providing molecular evidence of M . allardi using mitochondrial DNA barcode region (658bp) as well as mtCOI-3ʹ sequences (817bp). The study will help in understanding population genetics through diversity analysis, ecological role, and phenotypic structures associated with the geographic range of this species.
