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Porcentaje de aclimatación en plantas propagadas in vitro del híbrido almendro x durazno H1, en dos fechas y cuatro concentraciones de AIB. Con el fin de conocer la relación que existe entre los tratamientos empleados durante el enraizamiento y el porcentaje de aclimatación, se determinó la correlación entre ambas respuestas. En las dos fechas los coeficientes fueron altos y negativos (Figura 2), lo que indica que a mayor concentración de auxina durante el enraizamiento, menor será el porcentaje de aclimatación. Por tanto, no conviene utilizar concentraciones de auxina mayores a 0.1 µM durante el enraizamiento del híbrido H1. CONCLUSIONES La época de establecimiento in vitro influyó en la contaminación por hongos, porcentajes de brotación, sobrevivencia y muerte de explantes. El establecimiento
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The almond x peach hybrid H1 has the potential to be used as a peach (Prunus persica L.) rootstock, because it its highly resistant to drought, dry soils with low fertility, nematods, root asphyxia, and chlorosis induced by Fe deficiency. Since a hybrid propagated by seeds would produce segregation, for maintaining the desired plant traits the hybr...
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Murashige-Skoog (MS) or Lloyd and McCown (WPM) medium were used for micropropagation of Cissus tiliacea, an herb plant with potential in agriculture and pharmacology; in both medium similar results for shoot regeneration, number of leaves, leaves nodes, and adventitious roots were observed; only formation of callus was influenced (p ≤ 0.05) by the medium. For in vitro multiplication, benzyl adenine (BA) was added to WPM at 0, 0.5, 1.0, 1.5 or 2.0 mg L -1 and we used three types of explants that is basal, medium an upper. BA at 0 and 0.5 mg L -1 resulted in higher size and well developed plantlets; moreover, those concentrations allowed 1.2 or 1.6 roots per vitro plant. Those explants cultured in 1.5 or 2.0 BA resulted in callus development. Type of explant did not influence the evaluated explants responses. Rooting was evaluated in MS adding naphthalene acetic acid (NAA), indol butiric acid (IBA) or indol acetic acid (IAA) at 0.5 mg L -1; the higher number of secondary roots and the diameter of the main root was induced by NAA, but IBA resulted in longer main roots. Present results suggested that micropropagation of C. tiliaceae might be and alternative for conservation and multiplication of this wild plant.
