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Cultivar silvestre de Bromelia pinguin creciendo en Sinaloa. Planta joven (A), planta adulta durante llenado de fruto (B), racimo cosechado (C) y fruto maduro (D). Figure 1. Typical wild cultivar of Bromelia pinguin growing in Sinaloa. Whole young plant (A), mature plant during fruit filling (B), harvested raceme (C) and ripened fruit (D).
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Enzymatic extract from B. pinguin fruits were partially characterized by its milk-clotting activity, caseinolytic activities and inhibition studies. Raw enzymatic extract (REE) showed a maximum milk-clotting activity around 65-85ºC, corresponding to 3.99 Soxhlet units·mL-1 , indicating that 1 mL of REE is necessary to clot approximately 400 mL of m...
Context in source publication
Context 1
... conocida en como "aguama" o "guamara" (Bromelia pinguin L.) ha llamado la atención por la presencia de proteasas en su fruto. Esta especie posee hojas espinosas en rosetas con grandes vainas cubiertas de escamas y desar- rolla racimos de 40 cm con la capacidad de producir entre 40-50 frutos de 5 cm de longitud con un peso aproximado de 18.5 g (Fig. 1). Los frutos son sumamente apreciados en al- gunas regiones por sus propiedades nutritivas y medicinales (Payrol et al., 2005a;Pío-León et al., 2009) y existen algunas propuestas para su aprovechamiento en la preparación de bebidas (Camacho-Díaz et al., 2011). Sin embargo, el fruto sigue siendo subutilizado y/o desechado debido a la ...
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The study of fish digestive biochemistry is essential to understand factors that affect the net efficiency of food transformation and growth, and therefore aquaculture profitability. The aim of the present study was to assess the activity and functional characteristics of key digestive enzymes in juveniles of greater amberjack (Seriola dumerili), a...
Citations
... To 100 mL of the crude protease extract from cocuixtle pulp, 20% (p/v) of ammonium sulfate was added, while for the crude protease extract from guamara pulp, 30% (p/v) of ammonium sulfate was added considering the reported recommendations for each fruit (Romero-Garay et al., 2020;García-Magaña et al., 2018;Moreno-Hernández et al., 2017b), the dialyzed precipitates were lyophilized and named pre-purified proteases (PP). ...
Physiological and physicochemical behavior of guamara (Bromelia pinguin) and cocuixtle (Bromelia karatas) fruits, as well as the antibacterial effect of their pre-purified proteases
... karatas) with high specific proteolytic activity have been found similar to those reported for pineapple (García-Magaña et al., 2018;Meza-Espinoza et al., 2017); These fruits have been used since ancient times for their healing properties (Hornung-Leoni, 2011a, 2011b. Their proteases have important specific proteolytic activities (García-Magaña et al., 2018;Meza-Espinoza et al., 2017;Romero-Garay et al., 2020) and have been reported to have properties that can be used in the food and pharmaceutical industries (Ávalos-Arellano, López; Escandón-Rivera, Andrade-Ceto, & Sánchez-Villaseñor, 2019;Moreno-Hernández et al., 2017;Ruiz-Ruiz, Ramón-Sierra, Arias-Argaez, Magaña-Ortíz, & Ortíz-Vazquez, 2017). Considering these results, it is possible to hypothesize that the proteases present in the peel and seeds of B. pinguin and B. karatas could have similar properties. ...
The aim of this work was to evaluate and study the specific proteolytic activity, antioxidant, and anti-inflammatory capacity of proteolytic extracts obtained from peel and seeds of Bromelia pinguin and Bromelia karatas, as well as the effect of encapsulation on these properties. Proteolytic extracts of by-products from B. pinguin and from B. karatas with high specific proteolytic activity were encapsulated by electrospraying with agave fructans with a high degree of polymerization. The antioxidant and anti-inflammatory capacity of the capsule was analyzed in addition to its photostability. Encapsulation efficiency was assessed using scanning electron microscopy, thermogravimetry, and differential scanning calorimetry in the capsules of B. pinguin peel extracts with its high specific proteolytic activity. The antioxidant capacity and anti-inflammatory capacity decreases when the extract was encapsulated, showing stability during storage. With regard to the photostability of the capsules, the high degree polymerization agave fructans protected the compounds. The micrographs show capsules with spherical and smooth morphology, the encapsulation efficiency was 97%, and the glass transition temperature of the capsule was 160.5°C. These results open a window of possibilities in which the use of these encapsulated by-products can be considered in the food industry as well as in the pharmaceutical industry.
... Thus, the isolation and characterization of proteases from endemic plant sources are of great interest since they can be an alternative in the food and pharmacology industries. This is due to their stability under various physical and chemical conditions [17]. Therefore, the aim of this study was to isolate and purify proteases from B. karatas fruits and also compare them with proteases from B. pinguin fruits. ...
... Ammonium sulfate was added (20 and 30% w/v, respectively) to the CPE of B. karatas and B. pinguin [17]. The obtained precipitate was dialyzed through a cellulose membrane (D0530-30.8 ...
... The reported proteolytic activity of proteases from B. pinguin and B. karatas fruits is variable; for example, for proteases from B. pinguin extract, the reported values were 11 U cas /mL [6], 2.31 U cas /mg [8], 0.98 U cas /mL [16], 3.2 U/mg [17], while for proteases from B. karatas extract, the reported value was 1.2 azocol/mg min/mL [10]. In this work, the proteolytic activity of the concentrated proteases was 21.93 U T for proteases from B. karatas and 43.58 U T for proteases from B. pinguin. ...
The aim of this research is the partial characterization of proteases extracted from B. karatas; the isolation and purification of proteases from B. karatas fruits were achieved using precipitation, separation by size exclusion chromatography and anion-exchange chromatography; molecular mass (MM) was determined, and the effect of inhibitors, reducing agents and heat on enzyme activity was analyzed. These proteases were compared with proteases from Bromelia pinguin (B. pinguin) and evaluated under similar conditions. The isolation procedure was adequate; only a few protein bands are present in sodium dodecyl sulfate polyacrylamide gel electrophoresis. Furthermore, zymogram analysis showed protein bands with enzyme activity. Inhibitors, reducing agents and heat were unable to inactivate the proteases extracted from B. karatas and B. pinguin. The semi-purified extracts are a set of proteases with a MM of 66 kDa, but different isoelectric points (3.5–6.5 for B. karatas and 5–9 for B. pinguin), which are found in quaternary structures with proteolytic activity. When denatured, they segment into fragments of approximately 20 and 10 kDa. The data indicate that these plants could be used as sources of proteases since they present good proteolytic activity (21.93 UT for proteases from B. karatas and 43.58 UT for proteases from B. pinguin) and that B. Karatas has potential applications comparable to B. pinguin in the food and health industries.
