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A5_2). Group 5: photo 1-picta (captivity); photo 2-emma (captivity). 

A5_2). Group 5: photo 1-picta (captivity); photo 2-emma (captivity). 

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Introduction The relationships within the Pyrrhura species complex are partly unresolved. In this study, a comprehensive phylogenetic analysis of the Pyrrhura picta-leucotis complex was carried out, covering all species except P. subandina. Material and Methods We made a morphological analysis of 745 preserved specimens of all the taxa in differen...

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... It has been used by MW before for a phylogenetic reconstruction of many other bird taxa, including parrots (Kraus & Wink 2015). The PCR (polymerase chain reaction) amplifications were performed in 50 µl reaction volumes containing 1 × PCR buffer (Bioron, Ludwigshafen), 100 µM dNTPs, 0.2 units of Taq DNA polymerase (Bioron, Ludwigshafen), 200 ng of DNA and 5 pmol of primers for cytochrome b (as described inArndt & Wink 2017). Thermal cycling involved five minutes at 94°C, followed by 35 cycles of 40 seconds at 94°C, 40 seconds at 52°C, one minute at 72°C and a final extension at 72°C for ten minutes. ...
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... Seiner Revision des P. picta-leucotis-Komplexes sind allerdings nur del Hoyo & Collar (2014) gefolgt. Für die nun vorliegende Studie haben Arndt & Wink (2017) 745 Exemplare in Museen nahezu weltweit untersucht, mtDNA (cytb) der meisten Taxa sequenziert und in begrenztem Umfang auch Lautäußerungen (meist Flugrufe) ausgewertet. Auf der Basis dieses Materials legen sie eine überarbeitete Revision dieses Artenkomplexes vor. ...
... Dem BSC wird dieses Vorgehen allerdings nur in wenigen Fällen gerecht. Nach Arndt & Wink (2017) lassen sich die Sittiche des Pyrrhura picta-leucotis-Komplexes genetisch in sechs Gruppen mit insgesamt 15 Spezies gliedern: I. P. amazonum Hellmayr, 1906;P. pallescens Miranda-Ribeiro, 1926;II. ...
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... These individuals may represent intergrades with nearby populations that have varying amounts of bright or dark red on the forecrown (P. r. parvifrons, P. r. roseifrons, or P. orosaensis; Arndt 2008, Arndt andWink 2017). Further sampling and genetic analysis of these populations is needed to sort out taxonomic relationships. ...
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