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![FIGURE E Helvella rugosa. (a, b) Typical mature specimens [(a, b) Zhao oooo MFLUUU-----]. Scale bars. (a, b) = cm.](publication/372446880/figure/fig4/AS:11431281190657455@1695431349791/FIGURE-E-Helvella-rugosa-a-b-Typical-mature-specimens-a-b-Zhao-oooo-MFLUUU.png)
FIGURE E Helvella rugosa. (a, b) Typical mature specimens [(a, b) Zhao oooo MFLUUU-----]. Scale bars. (a, b) = cm.
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Most species of Helvella have been described from temperate regions in Asia, Europe, and North America, but little is known about the genus from tropical regions. In this report, phylogenetic analyses of 11 newly collected saddle-like fungi from northern Thailand using three genetic markers [the nuclear large subunit ribosomal DNA (LSU), the heat s...
Citations
... Species of Helvella L. are mainly distributed in forested regions of north-temperate eurasia and North america (Skrede et al. 2017. the genus comprised several edible and medicinal taxa, such as H. bachu Q. Zhao, Z. L. Yang & k.D. hyde, H. crispa Bull., and H. zhongtiaoensis J.Z. Cao & B. Liu , 2016, Yu et al. 2023. the macro-and micro-morphological features with taxonomic value include the shape, size, and color of pileus and stipe, stipitipellis, as well as the anatomy of the medullary excipulum, ectal excipulum, paraphyses, and ascospores (Dissing 1966, häffner 1987, abbott & Currah 1997. ...
... Collections were obtained in Yunnan Province, China. observations and photographs were prepared from material mounted in water and measurements followed the protocols previously outlined in Zhao et al. (2015) and Yu et al. (2023). the examined specimens were deposited in the herbarium of Cryptogams at kunming Institute of Botany, Chinese academy of Sciences (kuN-hkaS). the Facesoffungi numbers and Index Fungorum numbers were registered following the guidelines provided by Jayasiri et al. (2015) and Index Fungorum (2023). ...
... historically, the microanatomical characters of the Helvella is limited in the species delineation, and the recognition and classification of species within the Helvella genus were primarily based on macroscopic characteristics (Dissing 1966, abbott & Currah 1997, Skrede et al. 2017. the hymenium, excipulum, shape, color, and other surface characteristics of the cap and stipe have been used to distinguish and categorize different Helvella species (Skrede et al. 2017, Yu et al. 2023. however, morphological characteristics have inherent limitations as the main means of identifying and classifying Helvella in history. ...
During the investigation of Helvella in Yunnan, China, four saddle-like specimens were collected and studied. Through a comprehensive analysis of both morphological traits and phylogenetic data from LSU and hsp90 sequences, the four collections were identified and are described as two new species, i.e., Helvella liquii and H. pseudoatra. Helvella liquii stands out with its distinctive features, including the fusion of the pileus margin with the stipe, a slightly rugose coffee blackish surface on the receptacle, and relatively broad apex of the paraphyses. Helvella pseudoatra is characterized by a pubescent receptacle surface that exhibits shaded or dark grey to greyish brown. Additionally, the upwardly white to brown stipe with a basally white to greyish color further distinguishes this species. To provide a comprehensive understanding of these newly discovered taxa, detailed descriptions and illustrations of both Helvella liquii and H. pseudoatra species are accurately documented.
Helvella is a widespread, frequently encountered fungal group appearing in forests, but the species diversity and molecular phylogeny of Helvella in China remains incompletely understood. In this work, we performed comprehensive phylogenetic analyses using multilocus sequence data. Six datasets were employed, including a five-locus concatenated dataset (ITS, nrLSU, tef1-α, rpb2, hsp), a two-locus concatenated dataset (ITS, nrLSU), and four single-locus datasets (ITS) that were divided based on the four different phylogenetic clades of Helvella recognized in this study. A total of 1 946 sequences were used, of which 713 were newly generated, including 170 sequences of ITS, 174 sequences of nrLSU, 131 sequences of tef1-α, 107 sequences of rpb2 and 131 sequences of hsp. The phylogeny based on the five-locus concatenated dataset revealed that Helvella s. str. is monophyletic and four phylogenetic clades are clearly recognized, i.e., Acetabulum clade, Crispa clade, Elastica clade, and Lacunosa clade. A total of 24 lineages or subclades were recognized, 11 of which were new, the remaining 13 corresponding with previous studies. Chinese Helvella species are distributed in 22 lineages across four clades. Phylogenetic analyses based on the two-locus concatenated dataset and four single-locus datasets confirmed the presence of at least 93 phylogenetic species in China. Among them, 58 are identified as known species, including a species with a newly designated lectotype and epitype, 18 are newly described in this paper, and the remaining 17 taxa are putatively new to science but remain unnamed due to the paucity or absence of ascomatal materials. In addition, the Helvella species previously recorded in China are discussed. A list of 76 confirmed species, including newly proposed species, is provided. The occurrence of H. crispa and H. elastica are not confirmed although
both are commonly recorded in China.
