Fig 4 - uploaded by Degong Ruan
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Examples of recurrent acquired karyotypic abnormalities in hPSCs. Abnormalities can present with different sizes and mechanisms. Each aberration type is designated by a distinct color that outlines the chromosomal region gained or lost, and minimal overlapping regions (if known) are indicated by a darker color shade for cytobands 1q32, 12p13.3, 17q25, 18q21, and 20q11.21. Abnormalities are reported using ISCN: (a) +20, (b) i(20)(q10), (c) idic(20)(p12), (d) dup(20)(q11.2q11.2), (e) +12, (f) +i(12)(p10), (g) dup(12)(p13p13), (h) dup(1)(q21q44), (i) dup(1)(q25q32), (j) dup(1)(q32q32), (k) der(14)t(1;14)(q12;p11.2), (l) der(18)t(1;18)(q12; q21.1), (m) +X, (n) +8, (o) del(18)(q21.1), (p) del(18)(q21.1q21.3), (q) +17, (r) der(22)t(17;22)(q21.3;p11.2), (s) i(17)(q10)
Source publication
The development of porcine expanded potential stem cells (pEPSCs) provides an invaluable tool for investigation of porcine stem cell pluripotency and opens a venue for research in biotechnology, agriculture, and regenerative medicine. Since the derivation of pEPSC from porcine pre-implantation embryos has been demanding in resource supply and techn...
Citations
... Using our optimized medium, we have also been able to derive pEPSCs lines in opEPSCM. In this protocol, we describe the detailed procedure for reprogramming porcine somatic cells (OCT4-tdTomato PFF cells) 35 into pEPSC iPS using the previously described Dox-inducible piggyBac eight-factor reprogramming method 4,36 (Fig. 7a,b). The EPSC iPS lines could be efficiently established in opEPSCM ( Fig. 7c-e). ...
... Supporting data of this study can be found in our previous publications 4, 36 . All source data generated or analyzed during this study are included in this published article and its supplementary files. ...
... Moreover, modeling in nonhuman species further widens the tool-box for host and reservoir screening of emerging diseases [150,219], particularly in the context of zoonoses such as bornavirus disease, where animal models may possibly be closer to a reservoir species, and therefore represent an infection that is not relevant for human disease. Interestingly, next to mouse [222,223] and NHPs [224][225][226], iPSCs already exist for a wide range of domestic species, including pigs [227][228][229], horses [230,231], cattle [232,233], and sheep [234] (reviewed in [219]). Such models would present major advantages, particularly in emerging viruses such as borna-or filoviruses. ...
The order Mononegavirales contains a variety of highly pathogenic viruses that may infect humans, including the families Filoviridae, Bornaviridae, Paramyxoviridae, and Rhabodoviridae. Animal models have historically been important to study virus pathogenicity and to develop medical countermeasures. As these have inherent shortcomings, the rise of microphysiological systems and organoids able to recapitulate hallmarks of the diseases caused by these viruses may have enormous potential to add to or partially replace animal modeling in the future. Indeed, microphysiological systems and organoids are already used in the pharmaceutical R&D pipeline because they are prefigured to overcome the translational gap between model systems and clinical studies. Moreover, they may serve to alleviate ethical concerns related to animal research. In this review, we discuss the value of animal model alternatives in human pathogenic filovirus and bornavirus research. The current animal models and their limitations are presented followed by an overview of existing alternatives, such as organoids and microphysiological systems, which might help answering open research questions.
