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Entoloma kedrovense. Spores (a), basidium (b), cheilocystidia (c), and pileipellis (d). All figs from holotype. Bar = 10 µm.
Source publication
An account is given of some new and interesting Entoloma species collected in the Primorsky Territory of the Russian Far East. Six species (Entoloma eugenei, are new to science and their taxonomic position is discussed. In addition some interesting records of other species are documented.
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Citations
... The genus Entoloma (Fr.) P. Kumm. (1871: 23) mainly comprises the species with agaricoid basidiocarps; few species develop secotioid or gasteroid basidiocarps (Co-David et al. 2009;Kinoshita et al. 2012;Vidal et al. 2016) and is well-known for its pinkish brown spore print, and angular basidiospores in both face and polar views (Noordeloos 1980(Noordeloos , 1981(Noordeloos , 1984Singer 1986;Gates & Noordeloos 2007;Noordeloos & Hausknecht 2007;Noordeloos & Morozova 2010;Noordeloos et al. 2021). This is the second largest and species-rich genus of the order Agaricales and has a wide range of distribution ranging from the Arctic to the tropical (Pegler 1977;Largent 1994;Noordeloos 2004;Gates & Noordeloos 2007;Noordeloos & Hausknecht 2007;Co-David et al. 2009;Gates et al. 2009;Elliott et al. 2020;Dima et al. 2021). ...
In southern Punjab, Pakistan, the genus Entoloma remains poorly explored. Four collections of the genus Entoloma were collected during the mycological surveys from 2020 to 2022, in different areas of southern Punjab, Pakistan. Morpho-anatomical and molecular phylogenetic analyses based on ITS and combined ITS-28S sequences revealed that these four collections belong to the genus Entoloma Rusticoides group including a new species named E. khalidii and a new record for Pakistan, named E. phaeocarpum. The new species is characterized by a hemispherical to convex pileus with dark brown depressed center, slightly smaller isodiametric basidiospores, the presence of clavate to utriform cheilocystidia, and pileipellis hyphae with rounded ends. These findings underscore the importance of morphological, microscopical, and molecular approaches in fungal taxonomy and contribute to unveiling the fungal diversity in underexplored regions. The discovery of these species emphasizes the significance of continued exploration and conservation efforts to preserve myco-diversity in southern Punjab, Pakistan. Colored photographs of fresh basidiomata and habitats, illustrations of key anatomical characters, detailed descriptions, phylogenetic trees, and comparisons with closely related species are also provided.
... Entoloma (Fr.) P. Kumm. (1871: 23) is a species-rich genus and is well recognized by its pink spore print and angularity of basidiospores in both sides and end view (Noordeloos & Morozova 2010;Ediriweera et al. 2017;Wartchow & Braga-Neto 2019;Brandrud et al. 2020;Noordeloos et al. 2021). Most species are saprotrophic on soil and litter, while others are lignivores (Perraudin 2002), and some species, such as E. rhodopolium (Fr.) ...
A new species of Entoloma is described from Punjab, Pakistan. Species description is based on morpho-anatomical characterization of basidiomata and molecular phylogenetic analyses of ITS and 28S LSU region of nuclear ribosomal DNA. Entoloma punjabensis sp. nov. is distinguished by light gray to dark brown smooth pileus with crenate margins, reddish to brown central depression, larger globose to subglobose basidiospores 8.5-13.4 × 7.2-11.5 µm and hyaline hyphae of pileipellis.
... & O.V. Morozova is also close. It is recognized by the more robust basidiomata and presence of the cheilocystidia [21]. The p-distance (ITS1-5.8S-ITS2 ...
Four new species of Entoloma from Kon Chu Rang Nature Reserve and Ta Dung National Park were discovered during an investigation of the diversity of the mycobiota of Central Vietnam and are described here on the base of the molecular and morphological data. Phylogenetic analysis was based on nrITS1-5.8S-ITS2, nrLSU and tef1α regions. Illustrated descriptions of their macro- and microscopic features and discussion on similar taxa are given. Entoloma cycneum and E. peristerinum belong to the subgenus Cubospora. They are morphologically similar species and are characterized by white or whitish basidiomata with yellowish or beige tinges and with mainly smooth, glabrous, and hygrophanous pileus, longitudinally fibrillose or fibrillose-scaly white stipe, cuboid spores, and more or less cylindrical cheilocystidia, arising from hymenophoral trama. Entoloma peristerinum posseses initially more coloured beige conical pileus, discolouring to white with age and drying. The pileus of E. cycneum is initially white, hemisphaerical to convex, usually with thin pubescence near the margin. The species can be recognized also by the cheilocystidia form: serrulatum-type in E. cycneum vs. porphyrogriseum-type in E. peristerinum. Another two species belong to the subgenus Leptonia. Entoloma tadungense is close to E. percoelestinum from which it differs by smaller spores with pronounced angles, presence of the cheilocystidia, and the lilac discolouration of the stipe. E. dichroides is named after its similarity to E. dichroum, a dark blue coloured species with pronouncedly angled basidiospores. It is distinguished by the basidiospores form—irregularly 5(–6) angled with elongated apiculus, as well as by absence of the cheilocystidia and darker basidiomata with conical pileus. The article also describes the history of the study of the genus Entoloma in Vietnam with a list of 29 species mentioned in the publications for this country.
... While Australasia and temperate to boreal Eurasia were relatively well represented, few data were available for Africa, South America, and South Asia, and the data were incomplete for North America. In addition, Entoloma is especially species-rich in habitats with high conservation value (Horak 1978, Noordeloos & Hausknecht 1989, Noordeloos & Morozova 2010, Griffith et al. 2013, Noordeloos et al. 2017, while pristine habitats in tropical lowlands are often either destroyed or difficult to access. ...
Nolanea is a well-known and long-established subgenus of the genus Entoloma traditionally defined mainly by the mycenoid basidiocarps of the included species. Until now, revisions of this subgenus including molecular data exist only on a regional scale. In this study, the phylogeny of species of Nolanea is analysed based on multi-gene DNA sequences including data of specimens from all continents. New primers are designed for the mitochondrial small subunit and RPB2. The performance of the DNA loci in reconstructing the phylogeny in subg. Nolanea is evaluated. An ancestral state reconstruction is used to infer the character state evolution as well as the importance and reliability of morphological characters used to define subclades below subgeneric rank. Based on the results, seven sections are recognised in Nolanea: the sections Holoconiota, Infularia, Mammosa, Nolanea, Papillata, Staurospora, and the newly described sect. Elegantissima. A large phylogeny based on the fungal barcode rDNA ITS with numerous type sequences is used to evaluate current species concepts. Several names are revealed to be synonyms of older names. Four species new to science are described, namely E. altaicum, E. argillaceum, E. cornicolor, and E. incognitum. Lectotypes, epitypes or neotypes are designated for E. cetratum, E. clandestinum, E. conferendum, E. cuspidiferum, E. hebes, E. minutum, E. nitens, and E. rhodocylix. The re-evaluation of the limits of subg. Nolanea leads to an altered concept excluding species with distinct, lageniform cheilocystidia. The section Ameides is placed in subg. Leptonia. For several species formerly accommodated in Nolanea, but excluded now, viz., E. lepiotoides, E. rhombisporum, E. subelegans, and E. velenovskyi the taxonomic position remains unclear, because of the yet unresolved phylogeny of the whole genus Entoloma.
... make clear, that a coloured lamella is not of great diagnostic value in Cyanula. The collection from France (PAM00092901) has a blackish-brown lamella edge contrasting with the pale colours of pileus and stipe and was accordingly identified as E. atromarginatum (Noordeloos 2004). This taxon, described from peat bogs in the French Jura (Romagnesi & Favre 1938), was described and illustrated as a pale brown species, which may resemble discoloured specimens of E. calceus (Fig. 3E). ...
In anticipation of a phylogenetically revised monograph of Entoloma in Europe, six new species of subgenus Cyanula are described here. Entoloma cistocruentatum is associated with Cistus in Spain, E. dislocatum occurs in montane regions in Catalonia (Spain) and Tuscany (Italy), E. indikon is known from Denmark and three species are mainly distributed in the Nordic countries in Europe: E. calceus, E. perchalybeum and E. praecipuum. Entoloma incarnatofuscescens, from the /Rusticoides clade is neotypified. A fully amended description is given based on molecular evidence, which includes the recently described E. violaceoparkensis and E. klofacianum which became later synonyms.
Citation: Noordeloos ME, Vila J, Jordal JB, Kehlet T, Brandrud TE, Bendiksen E, Moreau P-A, Dondl M, Lorås J, Larsson E, Dima B (2022). Contributions to the revision of the genus Entoloma (Basidiomycota, Agaricales) in Europe: six new species from subgenus Cyanula and typification of E. incarnatofuscescens. Fungal Systematics and Evolution 9: 87–97. doi: 10.3114/fuse.2022.09.06
... 1), в то время G. punctatus и T. matsutake уже присутствуют в её действующей версии (Red Data Book… 2008). Entoloma eugenei в Приморском крае известна пока лишь из заповедника Кедровая Падь (Noordeloos, Morozova 2010). ...
... Microscopically, the sterile lamella edge composed of dense layer of clavate to subglobose and sphaeropedunculate cystidia is distinctive but, especially, in young specimens they can be mixed with cylindrical and lageniform cystidia. Entoloma subcaesiellum, described from the same region, is very similar morphologically, differing mainly in pileipellis structure (Noordeloos & Morozova 2010), but phylogenetically it is distinct. According to the molecular data, Entoloma ekaterinae belongs to the /chalybeum subclade of the /Cyanula clade. ...
Novel species of fungi described in this study include those from various countries as follows: Australia, Chaetomella pseudocircinoseta and Coniella pseudodiospyri on Eucalyptus microcorys leaves, Cladophialophora eucalypti, Teratosphaeria dunnii and Vermiculariopsiella dunnii on Eucalyptus dunnii leaves, Cylindrium grande and Hypsotheca eucalyptorum on Eucalyptus grandis leaves, Elsinoe salignae on Eucalyptus saligna leaves, Marasmius lebeliae on litter of regenerating subtropical rainforest, Phialoseptomonium eucalypti (incl. Phialoseptomonium gen. nov.) on Eucalyptus grandis × camaldulensis leaves, Phlogicylindrium pawpawense on Eucalyptus tereticornis leaves, Phyllosticta longicauda as an endophyte from healthy Eustrephus latifolius leaves, Pseudosydowia eucalyptorum on Eucalyptus sp. leaves, Saitozyma wallum on Banksia aemula leaves, Teratosphaeria henryi on Corymbia henryi leaves. Brazil, Aspergillus bezerrae, Backusella azygospora, Mariannaea terricola and Talaromyces pernambucoensis from soil, Calonectria matogrossensis on Eucalyptus urophylla leaves, Calvatia brasiliensis on soil, Carcinomyces nordestinensis on Bromelia antiacantha leaves, Dendryphiella stromaticola on small branches of an unidentified plant, Nigrospora brasiliensis on Nopalea cochenillifera leaves, Penicillium alagoense as a leaf endophyte on a Miconia sp., Podosordaria nigrobrunnea on dung, Spegazzinia bromeliacearum as a leaf endophyte on Tilandsia catimbauensis, Xylobolus brasiliensis on decaying wood. Bulgaria, Kazachstania molopis from the gut of the beetle Molops piceus. Croatia, Mollisia endocrystallina from a fallen decorticated Picea abies tree trunk. Ecuador, Hygrocybe rodomaculata on soil. Hungary, Alfoldia vorosii (incl. Alfoldia gen. nov.) from Juniperus communis roots, Kiskunsagia ubrizsyi (incl. Kiskunsagia gen. nov.) from Fumana procumbens roots. India, Aureobasidium tremulum as laboratory contaminant, Leucosporidium himalayensis and Naganishia indica from windblown dust on glaciers. Italy, Neodevriesia cycadicola on Cycas sp. leaves, Pseudocercospora pseudomyrticola on Myrtus communis leaves, Ramularia pistaciae on Pistacia lentiscus leaves, Neognomoniopsis quercina (incl. Neognomoniopsis gen. nov.) on Quercus ilex leaves. Japan, Diaporthe fructicola on Passiflora edulis × P. edulis f. flavicarpa fruit, Entoloma nipponicum on leaf litter in a mixed Cryptomeria japonica and Acer spp. forest. Macedonia, Astraeus macedonicus on soil. Malaysia, Fusicladium eucalyptigenum on Eucalyptus sp. twigs, Neoacrodontiella eucalypti (incl. Neoacrodontiella gen. nov.) on Eucalyptus urophylla leaves. Mozambique, Meliola gorongosensis on dead Philenoptera violacea leaflets. Nepal, Coniochaeta dendrobiicola from Dendriobium lognicornu roots. New Zealand, Neodevriesia sexualis and Thozetella neonivea on Archontophoenix cunninghamiana leaves. Norway, Calophoma sandfjordenica from a piece of board on a rocky shoreline, Clavaria parvispora on soil, Didymella finnmarkica from a piece of Pinus sylvestris driftwood. Poland, Sugiyamaella trypani from soil. Portugal, Colletotrichum feijoicola from Acca sellowiana. Russia, Crepidotus tobolensis on Populus tremula debris, Entoloma ekaterinae, Entoloma erhardii and Suillus gastroflavus on soil, Nakazawaea ambrosiae from the galleries of Ips typographus under the bark of Picea abies. Slovenia, Pluteus ludwigii on twigs of broadleaved trees. South Africa, Anungitiomyces stellenboschiensis (incl. Anungitiomyces gen. nov.) and Niesslia stellenboschiana on Eucalyptus sp. leaves, Beltraniella pseudoportoricensis on Podocarpus falcatus leaf litter, Corynespora encephalarti on Encephalartos sp. leaves, Cytospora pavettae on Pavetta revoluta leaves, Helminthosporium erythrinicola on Erythrina humeana leaves, Helminthosporium syzygii on a Syzygium sp. bark canker, Libertasomyces aloeticus on Aloe sp. leaves, Penicillium lunae from Musa sp. fruit, Phyllosticta lauridiae on Lauridia tetragona leaves, Pseudotruncatella bolusanthi (incl. Pseudotruncatellaceae fam. nov.) and Dactylella bolusanthi on Bolusanthus speciosus leaves. Spain, Apenidiella foetida on submerged plant debris, Inocybe grammatoides on Quercus ilex subsp. ilex forest humus, Ossicaulis salomii on soil, Phialemonium guarroi from soil. Thailand, Pantospora chromolaenae on Chromolaena odorata leaves. Ukraine, Cadophora helianthi from Helianthus annuus stems. USA, Boletus pseudopinophilus on soil under slash pine, Botryotrichum foricae, Penicillium americanum and Penicillium minnesotense from air. Vietnam, Lycoperdon vietnamense on soil. Morphological and culture characteristics are supported by DNA barcodes.
... Microscopically, the sterile lamella edge composed of dense layer of clavate to subglobose and sphaeropedunculate cystidia is distinctive but, especially, in young specimens they can be mixed with cylindrical and lageniform cystidia. Entoloma subcaesiellum, described from the same region, is very similar morphologically, differing mainly in pileipellis structure (Noordeloos & Morozova 2010), but phylogenetically it is distinct. According to the molecular data, Entoloma ekaterinae belongs to the /chalybeum subclade of the /Cyanula clade. ...
Coniochaeta dendrobiicola Sujit Shah, sp. nov.
Colour illustrations. Dendrobium longicornu orchid species from Chitlang
village, Makwanpur district, Nepal. Colony after 15 d on PDA, OA and CDA;
conidia, conidiogenous cells and hyphae. Scale bars = 10, 10 and 100 μm.
Sujit Shah, Bijaya Pant, Central Department of Botany, Tribhuvan University, Nepal; e-mail: b.pant@cdbtu.edu.np
Rohit Sharma & Yogesh S. Shouche, National Centre for Microbial Resource (NCMR), National Centre for Cell Science, India;
e-mail: rohit@nccs.res.in & yogesh@nccs.res.in
Jyotsna Sharma, Department of Plant and Soil Science, Texas Tech. University, USA; e-mail: jyotsna.sharma@ttu.edu
Etymology. Name reflects the host genus it was isolated from, Dendrobium
longicornu.
Classification — Coniochaetaceae, Coniochaetales, Sordariomycetes.
Vegetative hyphae thin, septate, smooth 1.2–2.4 μm wide.
Conidiogenous cells arising laterally from vegetative hyphae,
broader at base tapering towards apex (1.4 μm at base and
0.67 μm at apex). Conidia hyaline, smooth, cylindrical to allantoid,
variable in size, 4.35–11.28 × 1.2–2.3 μm. Sexual morph
absent which is reported in Coniochaeta velutina, C. prunicola,
C. africana isolated from Prunus (Damm et al. 2010, Weber
2002, Abdalla & Al-Rokibah 2003, Asgari & Zare 2006).
Cultural characteristics — Coniochaeta dendrobiicola was
first isolated on Czapek-Dox agar (CDA). The shape of the
colony was circular, with lemon yellow colour and pale regular
margin with pale white band as growing zone. The surface
was smooth with flat topography and submerged mycelium.
Colony 4 cm diam after 15 d of incubation, with 2–3 concentric
rings. On potato dextrose agar (PDA) the colony was circular
with regular margin, pale brown with yellowish margin having
radiating furrows. The surface was glistening, smooth with flat
topography and the presence of submerged mycelium. Colonies
reach 4 cm diam after 15 d of incubation, with 1–2 concentric
rings present. On oatmeal agar (OA) the colony shape was
circular with regular margin, lemon yellow with 1 cm thick white
growing margin. The colony surface was smooth, shiny with flat
topography and submerged mycelium. Colonies reach 4.5 cm
diam after 15 d of incubation, with a single concentric brown
ring present.
Habitat — Roots of Dendrobium longicornu, District Makwanpur,
Nepal.
Typus. Nepal, District Makwanpur, roots of Dendriobium lognicornu
(Orchidaceae), 25 May 2017, S. Shah (holotype culture and specimen,
MCC1811, preserved as metabolically inactive, ITS and LSU sequences
GenBank MK225602 and MK225603, MycoBank MB830652).
Notes — Phylogenetic trees of the ITS region was prepared
using sequences of C. dendrobiicola and other Coniochaeta
species obtained from GenBank. An NCBI BLASTn search
of ITS sequences showed closest similarity to be 93 % with
C. africana (CBS 120868, GenBank MH863095), 92 % with
C. velutina (STE-U 8315, GenBank KY312638), 92 % with
Coniochaeta angustispora (CBS 871.73, GenBank MH860816)
and 92 % with Coniochaeta nepalica (NBRC 30584, GenBank
LC146727).
... One of the simplest and distinct type of basidiospores in Entoloma s.l. is described as cuboid and it is recognized by six, more or less equal, quadrangular faces, two of which are the facets forming a dihedral base, one adaxial facet, a pair of lateral facets meeting in the apical adaxial position and an abaxial face (Pegler & Young 1978. A review of the taxonomic literature on cuboid basidiospores, mostly observed in optical microscopy, found them referred to in the following ways as: 'cuboid' (Romagnesi 1941, 1956, Dennis 1953, 1961, Horak 1973, 1977a, 1977b, 1982, Noordeloos 1980, 1992, Pegler 1983, Courtecuisse 1986, Hongo 1990, Baroni & Lodge 1998, Baroni & Halling 2000, Eyssartier et al. 2001, Manimohan et al. 2002, Natarajan & Ravindran 2003, Wartchow 2006, Noordeloos & Hausknecht 2007, Horak & Cheype 2008, Li et al. 2009, Noordeloos & Morozova 2010, Alves & Nascimento 2012, Largent et al. 2013a, He et al. 2015, Pradeep et al. 2016, Ediriweera et al. 2017; 'four-angled' where the four angles represent a rectangle in profile view (also called 'quadrate' or 'tetrahedral', Baker & Dale 1951, Hesler 1967, Horak 1976, 1977a, 1977b, 1982, Largent & Miller 1986, Largent 1994Halling & Mueller 2005, Largent et al. 2008, Aime et al. 2010, Anil Raj & Manimohan 2012, Pradeep et al. 2012, Coimbra et al. 2013, Largent et al. 2013a, 'cuboid-stellate' (Saccardo 1887, Coimbra et al. 2013, 'cuboid-cruciform' (Singer 1973, Horak & Desjardin 1993, 'quadrangular' (Saccardo 1891, Singer 1965, 'quadrate to rhomboidal' (Hesler 1967, Horak 1973, Eyssartier et al. 2001, 'twinnedtetrahedral' (Stevenson 1962, Horak 1973 and 'octahedral' (Murrill 1911). Thus, worldwide there are more than 120 species of the Entolomataceae described as having cuboid or cuboid-like basidiospores. ...
The generic or subgeneric delimitation by morphology of the Entolomataceae (Agaricales, Basidiomycota) is often based on the habit and external features of the basidiomata, the hyphal arrangement of the pileus surface and the shape of the basidiospores, which possess either bumps or undulate-pustules forming short ridges, or longitudinal ridges or are obviously angular with four to nine angles in profile view. Here, we examine the basidiospore shape of species in the /Entoloma clade described as cuboid to evaluate its importance in taxonomy using both phylogenetic and detailed analyses of the shape with Scanning Electron Microscopy. Our phylogenetic analyses support the placement of species with cuboid basidiospores into one of two clades. Based on this separation, two new subgenera of Entoloma are proposed: Cuboeccilia with an omphalinoid habit and fusoid cystidia and Cubospora which has a mycenoid to tricholomatoid habit and clavate, rarely fusoid cheilocystidia.
... Habitat -On soil in the flood plain forest. Comments -The specimens previously were identified as Entoloma violaceoserrulatum due to the absence of clampconnections (Noordeloos and Morozova 2010). Additional morphological examination allowed to find clampconnections in hymenium, and forced us to acknowledge that the identification had been erroneous. ...
Three new species of the gеnus Entoloma s.l. are described on the basis of morphological and molecular evidence. Two of them are morphologically and phylogenetically closely related to the European species E. violaceozonatum but differ sufficiently by their geographic distribution: E. bulakhae is from the Russian Far East and E. bidupense from Vietnam. The more distant E. atricolor from Vietnam is remarkable because of the brownish-black color of the basidiomata, blackish lamellae edge near the stem, and observed dimorphism in cheilocystidia. These three species are characterized by the combination of clamped basidia and a serrulatum-type lamellae edge and grouped together within the /Inocephalus–Cyanula clade in sister position to /Inocephalus and /Griseorubida. It is argued that they may possibly constitute a separate section in future, when more data will be available. For this reason, section Violaceozonata is proposed here “ad interim” to acknowledge the current isolated position.
