Fig 7 - uploaded by Sathish Sundararajan
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Embryogenic and nonembryogenic cells observed in established suspension cultures. a multi cell formation; b, c different shape of cells observed in suspension cultures; d cell population of stock suspension culture 7 days after subculture with starch grains; e cell clusters after sieving; f dead cells; Scale bar = 0.1mm
Source publication
The present investigation was carried out to establish a simple and efficient in vitro method for rapid propagation of Daucus carota L for different applications. Root, stem, leaf and seed explants were tested to induce callus on Gamborg medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D) for cell suspension culture establishment. The l...
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... culture 7 days after subculture with starch grains; e cell clusters after sieving; f dead cells; Scale bar = 0.1mm mostly vacuolated cells occur as small clusters showing rapid cell divisions. The seed derived suspension cultures, which showed highest of somatic embryo response, were used for the experiments. The capacity of proliferating (Fig. 7a), suspension cells to produce viable embryos became prominent from the third week of initiation. The cell cluster proliferation started slowly from undifferentiated cell clusters. Rapid cell division occurs in the following weeks from the embryogenic clusters, with the formation of globular embryos. The embryogenic (Fig. 7b, c) and ...
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... of proliferating (Fig. 7a), suspension cells to produce viable embryos became prominent from the third week of initiation. The cell cluster proliferation started slowly from undifferentiated cell clusters. Rapid cell division occurs in the following weeks from the embryogenic clusters, with the formation of globular embryos. The embryogenic (Fig. 7b, c) and non-embryogenic cells were observed clearly in cell clusters. Starch rich cells with a prominent cytoplasm (Fig. 7d) indicate the differentiation and proembryogenic cells as well as embryogenic calli were visualized ...
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... The cell cluster proliferation started slowly from undifferentiated cell clusters. Rapid cell division occurs in the following weeks from the embryogenic clusters, with the formation of globular embryos. The embryogenic (Fig. 7b, c) and non-embryogenic cells were observed clearly in cell clusters. Starch rich cells with a prominent cytoplasm (Fig. 7d) indicate the differentiation and proembryogenic cells as well as embryogenic calli were visualized ...
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... culture 7 days after subculture with starch grains; e cell clusters after sieving; f dead cells; Scale bar = 0.1mm mostly vacuolated cells occur as small clusters showing rapid cell divisions. The seed derived suspension cultures, which showed highest of somatic embryo response, were used for the experiments. The capacity of proliferating (Fig. 7a), suspension cells to produce viable embryos became prominent from the third week of initiation. The cell cluster proliferation started slowly from undifferentiated cell clusters. Rapid cell division occurs in the following weeks from the embryogenic clusters, with the formation of globular embryos. The embryogenic (Fig. 7b, c) and ...
Context 5
... of proliferating (Fig. 7a), suspension cells to produce viable embryos became prominent from the third week of initiation. The cell cluster proliferation started slowly from undifferentiated cell clusters. Rapid cell division occurs in the following weeks from the embryogenic clusters, with the formation of globular embryos. The embryogenic (Fig. 7b, c) and non-embryogenic cells were observed clearly in cell clusters. Starch rich cells with a prominent cytoplasm (Fig. 7d) indicate the differentiation and proembryogenic cells as well as embryogenic calli were visualized ...
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... The cell cluster proliferation started slowly from undifferentiated cell clusters. Rapid cell division occurs in the following weeks from the embryogenic clusters, with the formation of globular embryos. The embryogenic (Fig. 7b, c) and non-embryogenic cells were observed clearly in cell clusters. Starch rich cells with a prominent cytoplasm (Fig. 7d) indicate the differentiation and proembryogenic cells as well as embryogenic calli were visualized ...
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This study determined the effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.), the Thai rice landrace. Pakaumpuel's dehusked seeds were surface sterilized by using 20 % Sodium hypoclorite for 20 min before washed and cultured on Murashige and Skoog (MS) med...
Citations
... The carrot (Daucus carota L.) is a member of the Umbelliferae family, and it is a biennial plant. In addition to being consumed as food, different parts of the carrot can be used for medicinal purposes [2], such as hepatoprotective [3], antisteroidogenic [4], antinociceptive and anti-inflammatory functions [5]. The seeds are used for the treatment of swelling and tumors, and the roots are used as a poultice in mammary and uterine carcinoma as well as for skin cancer [1]. ...
... Stem explants showed the best induction of embryogenic and friable callus. It has been reported that stem explants are an excellent source for the production of somatic embryos from callus compared with all other explants [2,26]. Our results corroborate that stem explants present efficient callus formation and viable suspension cell cultures. ...
... Our results corroborate that stem explants present efficient callus formation and viable suspension cell cultures. It has also been reported that high concentrations of 2,4-D (above 1 mg/L) added to the culture medium developed different types of individual cells in the suspension cells of Daucus carota [2,27]. In this study, suspension cell cultures were established with embryogenic and friable calluses derived from stem explants. ...
The carrot is considered a model system in plant cell culture. Spray drying represents a widely used technology to preserve microorganisms, such as bacteria and yeasts. In germplasm conservation, the most used methods are freeze drying and cryopreservation. Therefore, the aim of this work was to evaluate the effect of spray drying on the viability and totipotency of so-matic carrot cells. Leaf, root and stem explants were evaluated to induce callus with 2 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D). Calli obtained from the stem were cultivated in a liquid medium with 1 mg/L of 2,4-D. Cell suspensions were spray dried with maltodextrin-gum Arabic and maltodextrin-xanthan gum mixtures, two outlet air temperatures (50 and 60 • C) and 120 • C inlet air temperature. Results showed that carrot cells were viable after spray drying, and this viability remained for six months at 8 • C. The totipotency of the microencapsulated cells was proven. Cells that were not spray dried regenerated 24.6 plantlets, while the spray dried cells regenerated 19 plantlets for each gram of rehydrated powder. Thus, spray drying allowed researchers to obtain viable and totipotent cells. This work is the first manuscript that reported the spray drying of plant somatic cells.
Somatic embryogenesis (SE) is a process where somatic embryos can form differentiated tissues and regenerate into new plants. Efficient SE and liquid culture system for large-scale production of banana cv. Chenichampa is presented. The embryo maturation media (M3) was modified with different organic additives and high-frequency somatic embryos were observed in M3 media supplemented with CH: Gln (100:150 mg/L) resulting in a tenfold increase in somatic embryo formation as compared to the control. The generated plantlets were hardened and polymerase chain reaction (PCR) with SSR primers were used to confirm the genetic fidelity of the plantlets. To understand the molecular mechanism of SE, expression patterns of transcript factors (TFs) in respect of embryogenic calli (EC) and embryogenic cell suspension (ECS) was also studied. Transcript factor (TF) such as BBM has been reported to play a significant part in converting explants to EC. The higher expression of TFs MaBBM1, MaBBM2, MaWUS2, and MaVP1 in the EC and MaBBM2 and MaWUS2 in ECS, suggested that these genes could play a crucial role in SE. The protocol developed in this commercially important banana cv. Chenichampa could be highly useful for large-scale micropropagation and genetic manipulation studies which can be adapted to other cultivars lacking in male flowers.
