Figure 12 - available via license: Creative Commons Attribution 4.0 International
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Egf family gene expression in ventral striatum of Bl6 and 129Sv mice. Two-way ANOVA was applied to demonstrate differences between the strains and environments. Substantial statistically significant gene expressions for (A) Egf-epidermal growth factor, (B) Nrg1-neuregulin 1, (C) Nrg3-neuregulin 3 and (D) Erbb1-epidermal growth factor receptor. Bonferroni post hoc test: * p ≤ 0.05 compared to respective 129Sv mice, ++ p ≤ 0.01, +++ p ≤ 0.001 and ++++ p ≤ 0.0001 in strain specific comparison. Data are expressed as mean values ± SD. Number of animals in each group varied from 7 to 11. Dorsal Striatum (Figure 13, Table S1)
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C57BL/6NTac (Bl6) and 129S6/SvEvTac (129Sv) mice display different coping strategies in stressful conditions. Our aim was to evaluate biomarkers related to different adaptation strategies in the brain of male 129Sv and Bl6 mice. We focused on signaling pathways related to the dopamine (DA) system, N-methyl-D-aspartate (NMDA) receptor and epidermal...
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... In qPCR, 8 dopamine-related genes were studied, tyrosine hydroxylase (Th), dopamine receptor 1 (Drd1), dopamine receptor 2 (Drd2), dopamine receptor 5 (Drd5), dopamine transporter (Dat), catechol-O-methyltransferase (Comt), monoamine oxidase A (MaoA), and monoamine oxidase B (MaoB). Dopamine system-related primers used in the experiment have been previously described by Varul et al. [51]. Additionally, two serotonin system-related genes were studied: serotonin transporter (Slc6a4) and tryptophan hydroxylase (Tph2). ...
In GWAS studies, the neural adhesion molecule encoding the neuronal growth regulator 1 (NEGR1) gene has been consistently linked with both depression and obesity. Although the linkage between NEGR1 and depression is the strongest, evidence also suggests the involvement of NEGR1 in a wide spectrum of psychiatric conditions. Here we show the expression of NEGR1 both in tyrosine- and tryptophan hydroxylase-positive cells. Negr1−/− mice show a time-dependent increase in behavioral sensitization to amphetamine associated with increased dopamine release in both the dorsal and ventral striatum. Upregulation of transcripts encoding dopamine and serotonin transporters and higher levels of several monoamines and their metabolites was evident in distinct brain areas of Negr1−/− mice. Chronic (23 days) escitalopram-induced reduction of serotonin and dopamine turnover is enhanced in Negr1−/− mice, and escitalopram rescued reduced weight of hippocampi in Negr1−/− mice. The current study is the first to show alterations in the brain monoaminergic systems in Negr1-deficient mice, suggesting that monoaminergic neural circuits contribute to both depressive and obesity-related phenotypes linked to the human NEGR1 gene.
... They also demonstrated that this damage can be prevented by inhibition of nitric oxide synthase (NOS) [10]. Moreover, we have previously shown that LPS-treated Bl6 mice have elevated plasma levels of endogenous NOS inhibitors ADMA and SDMA [4]. ...
... It has been demonstrated repeatedly that Bl6 and 129Sv mice display clearly different coping strategies in response to different stressors. Bl6 mice have an active coping strategy in stressful situations, whereas the coping strategy of 129Sv strain is considered passive [4]. Furthermore, we have previously demonstrated that Bl6 mice seem to cope actively with inflammation by inducing a stronger hypometabolic state, whereas the metabolism of 129Sv mice appears to enhance the proinflammatory status [6]. ...
Many studies have demonstrated significant mouse-strain-specific differences in behavior and response to pathogenic and pharmacological agents. This study seeks to characterize possible differences in microglia activation and overall severity of neuroinflammation in two widely used mouse strains, C57BL/6NTac (Bl6) and 129S6/SvEvTac (129Sv), in response to acute lipopolysaccharide (LPS) administration. Locomotor activity within the open field arena revealed similar 24 h motor activity decline in both strains. Both strains also exhibited significant bodyweight loss due to LPS treatment, although it was more severe in the Bl6 strain. Furthermore, LPS induced a hypothermic response in Bl6 mice, which was not seen in 129Sv. We found that 24 h LPS challenge significantly increased the inflammatory status of microglia in 129Sv mice. On the other hand, we observed that, under physiological conditions, microglia of Bl6 seemed to be in a higher immune-alert state. Gene and protein expression analysis revealed that LPS induces a significantly stronger upregulation of MHC-I-pathway-related components in the brain of Bl6 compared to 129Sv mice. The most striking difference was detected in the olfactory bulb, where we observed significant LPS-induced upregulation of MHC-I pathway components in Bl6 mice, whereas no alterations were observed in 129Sv. We observed significant positive correlations between bodyweight decline and expressions of MHC-I components in the olfactory bulbs of Bl6 mice and the frontal cortex of 129Sv, highlighting different brain regions most affected by LPS in these strains. Our findings suggest that the brains of Bl6 mice exist in a more immunocompetent state compared to 129Sv mice.
