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Effects of rapeseed oil fortified with micronutrients (polyphenols, tocopherols and phytosterols) on TG, TC and HDL-C in plasma of rats fed a high-fat diet. RRO: the refined rapeseed oil group; L-. M- and H- FRRO: fortified refined rapeseed oil with low, middle and high contents of micronutrients groups. Bars represent the mean ± SD from 10 animals in each group. * p < 0.05 and ** p < 0.01 compared to the RRO group.
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Micronutrients polyphenols, tocopherols and phytosterols in rapeseed exert potential benefit to cardiovascular system, but most of these micronutrients are removed by the refining process. The aim of this study was to determine the effect of rapeseed oil fortified with these micronutrients on the atherosclerosis risk factors in rats fed a high-fat...
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Intake of high-fat diet is associated with increased fatty livers. Hepatic lipid accumulation and oxidative stress are key pathophysiological mechanisms in this disease. Micronutrients polyphenols, tocopherols and phytosterols in rapeseed exert potential benefit to hepatoprotection, but most of these micronutrients are removed by the traditional re...
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... Napier and Graham, (2010) have reported the promising approach of transgenic metabolic engineering in developing transgenic plant producing designer oil enriched with omega-3 long chain PUFA equivalent to the level found in marine organisms. Recently, Xu et al. (2011) concluded from their experiments that designer oil based approach for rapeseed oil enriched with micronutrients such as polyphenols, tocopherols and phytosterols may be effective in the prevention of atherogenesis Phenol enrichment of olive oils (Suárez et al., 2010) and palmitic acid-fortified vegetable oil produced synergistic effects on calcium absorption and it is beneficial for baby foods including infant formula, with regard to increasing absorption of calcium by higher soluble calcium in the small intestinal content (Lee et al., 2008). Eshigina et al. (2007) investigated the influence of dietary therapy containing sunflower oil with phospholipids (PL) on the lipid profile of plasma and composition of fatty acids of red blood cells in patients with hypertension and obesity and observed the reduction in serum total cholesterol, low density lipoprotein (LDL), apo-lipoprotein A 1, apo-B and fibrinogen. ...
During the past decade the knowledge of dietary influence on health and wellbeing has amply been increased and often related to some food components. To ameliorate specific nutritional disorders, designer foods have been ad vented way back in 1980.Designer foods with potential health promoting food ingredients, which are normal foods consumed daily. The designer food approach is one of the best and low among people, be are developed by the process of fortification or bio the potential health benefits in alleviating life style and chronic disord foods are reviewed in this communication. In developed and developing countries, the designer foods played a major role in improving the diet and eliminating life style and chronic disorders and nutrient deficiencies among peopl biotechnological methods like transgenic plants have health value. Designer rape seed oil and sunflower oil can alleviate lipid profiles in hyper tension and obesity patients. Desig like broccoli sprouts and cereals like fermented rice with monascus have reported to have antioxidant and immune wheat etc are fortified with beta caro foods are developed and available now in the market. Polyphenol fortified rich foods like tea are proved to have potential health benefits. As of now, designer foods are completely safe but fortification or bio recommended daily allowances of nutrients and they should be strictly regulated with stringent quality control measures to ensure safety and acceptance before success of designer foods depends on creating adequate awareness among people about their health benefits through national and international programs as well.
... As a result of the study, after the consumption of oil in rats, the levels of triglycerides and plasma cholesterol decreased markedly. Thus, rapeseed oil prevents atherogenesis by improving plasma oxidative stress, the lipid profile and inflammation [103]. ...
... Antidiabetic properties The peptides present in rapeseed affect high levels of insulin secretion 680 µg/mL [84] Hypoglycemic action An extract derived from rapeseed significantly reduces serum triglyceride levels 3 g/kg [92] Atherosclerosis Rapeseed oil prevents atherosclerosis by lowering plasma triglyceride and cholesterol levels 20% [103] Antioxidant activity The methanol extract of rapeseed has an antioxidant effect 1000 µl [105] Antiviral activity An aqueous extract of rapeseed shows antiviral activity against the A/H1N1 influenza virus 0.5 mg/mL [110] Hepatitis C virus ...
Rapeseed (Brassica napus L.) is a herbaceous annual plant of the Cruciferous family, the Cabbage genus. This oilseed crop is widely used in many areas of industry and agriculture. High-quality oil obtained from rapeseed can be found in many industrial food products. To date, extracts with a high content of biologically active substances are obtained from rapeseed using modern extraction methods. Brassica napus L. seeds contain polyunsaturated and monounsaturated fatty acids, carotenoids, phytosterols, flavonoids, vitamins, glucosinolates and microelements. The data in this review show that rapeseed biocompounds have therapeutic effects in the treatment of various types of diseases. Some studies indicate that rapeseed can be used as an anti-inflammatory, antioxidant, antiviral, hypoglycemic and anticancer agent. In the pharmaceutical industry, using rapeseed as an active ingredient may help to develop new forms drugs with wide range of therapeutic effects. This review focuses on aspects of the extraction of biocompounds from rapeseed and the study of its pharmacological properties.
... The levels of creatine kinase-MB (CK-MB), Troponin I (cTnI) and lactate dehydrogenase (LDH) were detected by the corresponding commercial kits (Sangon Biotech, Shanghai, China) based on the instruction of manufacturer. An Automatic Agarose Gel Electrophoresis Apparatus (Epalyzer 2; Helena Laboratories, Saitama, Japan) was operated in determination of triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and highdensity lipoprotein cholesterol (HDL-C) in serum, Enzymelinked immuno sorbent assay (ELISA, Thermo Fisher Scientific Co., Ltd., China) kits to determine the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and monocyte chemotactic protein-1 (MCP-1) in plasma (Abcam, Cambridge, MA, USA), while for the activity of nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA), previously published methods were used [22][23][24]. ...
To investigate the efficacy of Slit2 in the rats with coronary heart disease (CHD). CHD model were constructed by feeding high-fat food and injecting with pituitrin in rat, followed by recombinant Slit2 treatment, and then the cardiac function was evaluated by echocardiography, and the indicators concerning the cardiomyocyte injury markers and lipoprotein status and oxidative stress were measured. The Slit2 expression in the heart tissues was identified by immunofluorescence. Enzyme-linked immunosorbent assay (ELISA) was carried out to detect inflammatory cytokines, H2DCFDA staining to determine the ROS generation in heart tissues, Masson trichrome staining to observe myocardial fibrosis, and qRT-PCR and Western blotting to detect gene and protein expressions. Slit2 decreased the levels of LDH, CK-MB, cTnI, TG, TC and LDL-C and increased HDL-C level in CHD rats. In the normal heart tissues, Slit2 expression was significantly lower in cardiomyocytes than cardiac fibroblasts. Furthermore, the expressions of VCAM-1, ICAM-1, fibronectin and TGF-β1 were increased in the heart tissues of CHD rats with the obvious myocardial fibrosis, which were dose-dependently reversed by recombinant Slit2. In addition, recombinant Slit2 also dose-dependently increased the activity of NO, SOD, CAT and GSH-Px, and decreased TNF-α, IL-6, MCP-1, MDA and ROS in CHD rats. Slit2 was downregulated in myocardial tissue and plasma of CHD rats. Recombinant Slit2, by regulating the level of blood lipid, can relieve the myocardial fibrosis, inflammation and oxidative stress in CHD.
... It represents a healthier version of traditional fresh spreadable cheese produced only of milk fat. A similar tendency was observed by other authors [33][34][35]. An important characteristic of the end product is its sensory profile. ...
Introduction. This research aims at determining the effect of canola oil and natural antioxidant of basil on chemical and sensory properties of fresh cheese.
Materials and methods. A fresh spreadable cheese was prepared by the use of fresh whole milk, canola oil and natural antioxidant of basil. The prepared cheese was analysed on fatty acid profile according to GC method of methyl esters, tocopherol content – according to HPLC method, phytosterol content – according to GC method, health lipid indices – according to empirical calculations complying with the fatty acid profile of the product, sensory evaluation – according to 10-points hedonic scale.
Results and discussion. Canola oil was characterized by a high content of unsaturated fatty acid content above 93 g.100 g-1 of the total content and a very good ratio of omega-6/omega-3 – 2/1. The total content of tocopherols in the lipid fraction was comparatively high – 315 mg.kg-1. Canola oil contained significant amount of phytosterols – 0.7 g.100 g−1 with individual sterol composition – β-sitosterol (54.7 %) and campesterol (35.3 %) predominated in the sterol fraction.
The cheese with partial replacement of milk fat by canola oil was characterized by a reduced content of saturated fatty acids which have negative effects on the human health – 35.18 g.100 g-1 compared to 68.33 g.100 g-1 in cheese produced by classical technology containing only milk fat. The product contained a large part of the short-chain fatty acids, typical for milk fat that defines the functionality of the end product. The cheese with added canola oil presented a higher biological value due to atherogenic index of 0.42 compared to 1.60 for cheese produced only of milk fat, thrombogenic index of 0.59 compared to 3.13 for cheese produced only of milk fat and preventive lipid score of 34.89 compared to128.23 for cheese produced only of milk fat.
The fresh cheese with partial replacement of milk fat by canola oil had good sensory properties.
Conclusions. Fresh spreadable cheese with partial replacement of milk fat by canola oil in the ratio (1:1) was characterized by increased healthy characteristics – low levels of atherogenic and prothrombogenic index and preventive lipid score.
... These results may be attributed to efficacy of used oil as antihistaminic, anti-inflammatory, high vitamin E and antioxidant activity. These findings are consistent with the result recorded by (Rabinkov et al., 1998;Miron et al., 2000;Xu et al., 2011;Pillaiyar et al., 2017;Qing, 2017). Moreover, essential oil had been approved as food additives and fall in the category of generally recognized as safe by the US FDA (Bilsland and Strong, 1990). ...
The present study was carried out to evaluate the effect of plant essential oils on Dermanyssus gallinae (D. gallinae). In vitro six groups of red mites, 20 mites in each group were exposed to direct spray of combined plant essential oils (Alisal) in rate of 0.25% on mites. activity and changes under stereomicroscope showed that sprayed mites completely stopped movements at both 1-and 2-h after treatment with completely stretched legs and white bead-like spots of oils accumulation on legs and bodies at 1-and 2-h. while, the non-treated mites were active with pale light brown colour. In vivo effect of Allisal to control red mite infestation in laying hens was investigated. In case of drinking water method mite count reduction rate on the bird was 60 %, 10%, and 0% as well as 0%, 0% and 10% in their traps at 4,7, and 12 days respectively, from the start of treatment in drinking water. While, in spray method mite reduction was 40%, 20%, and 10% on birds and 0%, 0% and 30% in the traps. On the other hand, water intake, feed intake, general health condition, skin health, and feather condition scores were improved at 4 th day post treatment (DPT). Total lesion score at 12 DPT was improved. The present study concluded that in vitro combined plant essential oils have rapid and strong acaricide effect in contact sprays. In vivo, there was obvious improvement in groups treated with plant oils than non-treated group. Drinking water treated birds showed good results than spray treated group. Therefore, it is recommended to use combined plant essential oils in D. gallinae control strategies in poultry.
... All the hematological values returned to the normal values after treatment with essential oils mixture indicating the protective and repairing effect of the studied essential oil mixture which supported by of garlic parts have antioxidant properties shown to be effective in reducing tributyltin (TBT) induced oxidative damage in vivo and in vitro (Liu and Xu, 2007) and had tissue protection against nicotine-induced oxidative damage (Augusti and Mathew, 1973;Fukao et al., 2004). Also, studies showed that Rapseed oil (canola oil) which is one of the studied oils mixture, contains different antioxidant substances (Vuorela et al., 2004;Loganes et al., 2019), vitamin E (Xu et al., 2011), with wide range variability in content and composition within rapeseed variees (Seker et al., 2008), carotenoids and phenolic compounds (Chen et al., 1996;Basta et al., 2011). Vitamin E as one of natural an-oxidant and radical scavenger is able to maintain the cellular integrity and promote the prevention and treatment of several inflammatory diseases (Combs and McClung, 1992;Morrissey et al., 1994). ...
... All garlic components used in our oil mixture reported to be effective (Rabinkov et al., 1998;Miron et al., 2000;Fukao et al., 2004;Kuete, 2017;Satyal, 2017). Rapseed oil contains different anoxidant substances (Vuorela et al., 2004;Loganes et al., 2019), Vitamin E (Xu et al., 2011;Seker et al., 2008), carotenoids and phenolic compounds (Chen et al., 1996;Basta et al., 2011). The rosehip-seed oil has antioxidant activity (Grajzer et al., 2015;Ilyasoglu, 2017;Mármol et al., 2017;Da Silva et al., 2019), hepatoprotective, antioxidant and anti-inflammatory properties in kidneys disorders were described (Ashtiyani et al., 2013;Sadeghi et al., 2016;Zuk and Bonventre, 2016). ...
In this study essential oils mixture (Lacecca®) was used for treatment (control) and prevention of ticks
infestation in dogs (0.25 ml/kg b.wt) orally followed by water for 3 successive days followed by 4 weeks observation
period. In infested oral treated dogs, ticks and skin affections (irritation and inflammation) were decreased and
disappeared gradually by treatment. Alive tick number was decreased to 75% at 6 hours from the 1st dose to reach 99.42
% at 6 hours from the 3rd dose and 100% starting from 12 hours from the 3rd dose up to 28 days. Complete disappearance
of ticks from tested dogs bodies at 7, 14 and 21 days after treatment. Oral dosing of essential oils mixture (Lacecca®)
resulted in protection (100%) of dogs against ticks infestation, complete safety and no adverse effect on control and
preventive group. Ticks infestation showed adverse effects on hematological values, decrease in Hb% (12.40±0.55),
PCV % (37.13±1.47), TEC 106/ml 5.29±0.03 and increase in total leucocytic count (TLC 103/ml) 22.64±2.88 in
infested dogs than in normal dogs. Infested treated group showed decrease in glucose (77.60±7.64mg/dl) and albumin
(2.20±0.01g/l). Increase of total protein (7.79±0.03g/l), ALT (64.10±2.23 IU/l), AST (73.20±5.13IU/l) and ALP
(140.10±2.07 IU/l00) levels than in normal dogs. Treatment with essential oils mixture improved health condition of
tick infested dogs where all blood values and biochemical parameters returned to normal values. Preventive treatment
with Lacecca® showed no bad changes in hematological values and biochemical parameters and it is safe in healthy
and preventive group. The obtained results proved that essential oils mixture is safe, has no adverse effects and has high
efficacy in both control (treatment) and prevention of tick infestation in dogs. Therefore, this study pointed out that
essential oils mixture is a very promising safe and acaricidal agent for control and prevention of tick infestation in dogs.
... Based on the results of the S-plots and volcano plots, the comparison between Chinese and Canadian rapeseeds was performed, and 67 metabolites out of 152 were identified as differential metabolites based on the criteria. The 67 metabolites, as listed in Table S2, include phenylpropanoids (12), flavonoids (11), terpenoids (15), alkaloids (2), amino acids (2), amino acid-related compounds (4), fatty acids (11), lipids (2), organic acids (2), polyketides (3), and others. Compared to Canadian rapeseeds, 40 metabolites were up-regulated and 27 metabolites were down-regulated in Chinese rapeseeds. ...
Rapeseed is an important oilseed with proper fatty acid composition and abundant bioactive components. Canada and China are the two major rapeseed-producing countries all over the world. Meanwhile, Canada and Mongolia are major importers of rapeseed due to the great demand for rapeseed in China. To investigate the metabolites in rapeseeds from three countries, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based metabolomics was employed to analyze rapeseeds from China, Canada, and Mongolia. As results, 67, 53, and 68 metabolites showed significant differences between Chinese and Canadian, Chinese and Mongolian, and Canadian and Mongolian rapeseeds, respectively. Differential metabolites were mainly distributed in the metabolic pathways including phenylpropanoid biosynthesis, flavone and flavonol biosynthesis, and ubiquinone and other terpenoid-quinone biosynthesis. Among the differential metabolites, contents of sinapate and sinapine were higher in Chinese rapeseeds, while the contents of brassicasterol, stigmasterol, and campestanol were higher in Canadian rapeseeds. These findings might provide insight into the metabolic characteristics of rapeseeds from three countries to guide processing and consumption of the products of rapeseed.
... In addition to enzymatic antioxidative factors, the protective system also comprises non-enzymatic antioxidants soluble in water and fats, such as vitamin E, β-carotene, and vitamin C, polyphenols or other compounds such as squalene. They are delivered through a suitable diet and improve the body's enzymatic antioxidant system (7,23). One way of supplying these components is consuming vegetable oils. ...
Introduction: The aim of this study was to investigate whether the type and form of oil (raw/non-oxidised (N) or post-frying/oxidised (O)) consumed in high-fat diets affect the oxidative status of an organism, as observed by malondialdehyde (MDA) concentration as an oxidative factor and antioxidant enzyme activity.
Material and Methods : Fats in the diet came from rapeseed oil (R) and olive oil (O).
Results: The applied diet caused a decrease in MDA concentration (μmol/L) in serum in group RN from 2.94 ± 0.87 to 1.76 ± 0.13, in group ON from 2.45 ± 0.62 to 1.50 ± 0.10, and in group OO from 2.70 ± 1.16 to 1.84 ± 0.36. Meanwhile, MDA concentration (mmol/L) increased in blood haemolysate in group RO from 0.15 ± 0.07 to 0.22 ± 0.03 and in group OO from 0.17 ± 0.02 to 0.22 ± 0.02. The observed changes caused a response of the enzymatic antioxidant system in both models, especially followed by an increase in activities of total superoxide dismutase and its mitochondrial isoenzyme in all experimental groups, while its cytosolic isoenzyme activity increased only in ON and OO groups. Increased activity of glutathione peroxidase (GPX) in groups RN and RO and of catalase (CAT) in groups ON and OO was observed. Significant differences in responses to the different types and forms of oils were probably caused by the different oxidative stability of the studied oils.
Conclusion: This diet disturbed the body's oxidative status; however, during the six-month study the enzymatic antioxidant system remained effective.
... Rapeseeds contain bioactive compounds including antioxidant vitamins such as tocopherol (mainly alpha tocopherol), phenolic molecules (canolol, sinapic acid, sinapine), coenzyme Q (CoQ) and phytosterols. These micronutrients have healthy metabolic, antiinflammatory and physiologic effects [6][7][8][9][10]. The intake of fatty acid (notably PUFA) increases the rate of fatty acid oxidation leading to peroxisomal and mitochondrial hydrogen peroxide production. ...
... However, the fortification with α-tocopherol, CoQ and canolol (group RFC) had a beneficial effect on triacylglycerol and fasting plasma glucose despite similar changes in the accumulation of ALA elongation/desaturase products (EPA and DHA) compared to groups R and RF in different tissues. The decrease in plasma triacylglycerol level confirms the results obtained in previous studies on the effect of fortified rapeseed oil [6,7]. Such an effect could be related to changes in the fate of FA in adipose tissue. ...
Background
Better choices of dietary lipid sources and substitution of refined by fortified oils could reduce the intake of saturated fatty acids (FA) and increase the intake of omega 3 FA concomitantly to healthy bioactive compounds.
Methods
The development of obesity and metabolic disturbances was explored in rats fed during 11 weeks with a high fat diet (HFD) in which the amount of saturated and polyunsaturated FA was respectively reduced and increased, using rapeseed oil as lipid source. This oil was used in a refined form (R) or fortified (10 fold increase in concentration) with endogenous micronutrients (coenzyme Q10 + tocopherol only (RF) only and also with canolol (RFC)). The effect of substituting palm by rapeseed oil was analysed using a student t test, oil fortification was analysed using ANOVA statistical test.
Results
Despite a similar weight gain, diets R, RF and RFC improved glucose tolerance (+ 10%) of the rats compared to a standard HFD with palm and sunflower oils as lipid source. Plasma glucose was lowered in RF and RFC groups (− 15 and 23% respectively), although triacylglycerol level was only reduced in group RFC (− 33%) compared to R. The fortification with canolol promoted the activation of Akt and AMP-activated protein kinase (AMPK) in skeletal muscle and subcutaneous adipose tissue respectively. Canolol supplementation also led to reduce p38 MAPK activation in skeletal muscle.
Conclusions
This study suggests that the presence of endogenous micronutrients in rapeseed oil promotes cellular adaptations to reverse glucose intolerance and improve the metabolism of insulin sensitive tissues.
Electronic supplementary material
The online version of this article (10.1186/s12986-018-0259-x) contains supplementary material, which is available to authorized users.
... hydroxytyrosol) [43,55,56]. Indeed, the high concentration of polyphenols is often associated with the anti-inflammatory and antiatherogenic actions of oils rich in monosaturated fatty acids such as olive oil and rapeseed oil [57][58][59]. ...
Atherosclerosis, a chronic inflammatory disorder of medium and large arteries and an underlying cause of cardiovascular disease (CVD), is responsible for a third of all global deaths. Current treatments for CVD, such as optimized statin therapy, are associated with considerable residual risk and several side effects in some patients. The outcome of research on the identification of alternative pharmaceutical agents for the treatment of CVD has been relatively disappointing with many promising leads failing at the clinical level. Nutraceuticals, products from food sources with health benefits beyond their nutritional value, represent promising agents in the prevention of CVD or as an add-on therapy with current treatments. This review will highlight the potential of several nutraceuticals, including polyunsaturated fatty acids, flavonoids and other polyphenols, as anti-CVD therapies based on clinical and pre-clinical mechanism-based studies.
