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Effects of DEHP on the vascular area of yolk sac vascellum in chick embryos. (A)The yolk sac vascellum was observed in chick embryos treated with DEHP (10-6 M) and/or nutrients, respectively. (B) The histogram represents the relative percent of the vascular area of yolk sac vascellum in chick embryos treated with DEHP and/or nutrients. * P < 0.05, compared with control (administration of saline, FA, Fe, CHO or DEHP alone), or * * P < 0.01, compared with control (administration of saline).
Source publication
Diethylhexyl phthalate (DEHP) is known as a persistent environmental pollutant. However, the possible effects of DEHP on human neural tube defects (NTDs) remain elusive. We set out to investigate the exposure of DEHP in human and explore the association of DEHP and NTDs. The level of DEHP in maternal urine was measured and analyzed by GC-MS. To fur...
Contexts in source publication
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... effect of DEHP on the development of yolk sac vascellum was analyzed by vascular area (Fig. 2). The vascular area was significantly decreased in DEHP-treated chick embryos, compared with control ( Fig. 2, P < 0.01), implying that yolk sac vascellum development was injured by DEHP exposure. ...
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... effect of DEHP on the development of yolk sac vascellum was analyzed by vascular area (Fig. 2). The vascular area was significantly decreased in DEHP-treated chick embryos, compared with control ( Fig. 2, P < 0.01), implying that yolk sac vascellum development was injured by DEHP exposure. ...
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... that the fluorescence signals of ROS was increased 2.8 fold after DEHP treatment in chick embryos compared with the control (Fig. 3), implying that DEHP could lead to the production of oxidative stress in chick embryos. The result was similar from the analysis of fluorescence signals of ROS in brain, heart and spine of chick embryos, respectively (Fig. 3 B2−B4). Oxidative stress indicators, including SOD, MDA and GPX, were also altered following DEHP treatment. The total SOD activity was dramatically reduced in chick embryos treated by DEHP (P < 0.05), as compared with the control (Fig. 4). Cu-Zn SOD activity was also obviously decreased when DEHP (P < 0.01) was administered to chick embryos. ...
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... and TUNEL assay. As shown in Fig. 5, TUNEL-positive signals were obviously elevated in whole embryo after DEHP treatment, compared with the control (P < 0.01; Fig. 5), which implied that DEHP may affect apoptosis. The result was similar from the analysis of TUNEL-positive signals in brain, heart and spine of chick embryos, respectively (Fig. 5 ...
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... reverse effect on the embryo survival, embryo body weight and embryo malformation in DEHP-treated chick embryos (Table 3). CHO (100 μg/μl) could partially reverse these effects (Table 3). Moreover, the vascular area of yolk sac vascellum was obviously increased in DEHP-treated chick embryos by CHO (100 μg/μl) supplement compared with control ( Fig. 2, P < 0.01). However, no remarkable reverse effect was observed with the supplement of Fe (20%) and FA (10 μg/μl) on the vascular area of DEHP-treated chick embryos (Fig. ...
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... effects (Table 3). Moreover, the vascular area of yolk sac vascellum was obviously increased in DEHP-treated chick embryos by CHO (100 μg/μl) supplement compared with control ( Fig. 2, P < 0.01). However, no remarkable reverse effect was observed with the supplement of Fe (20%) and FA (10 μg/μl) on the vascular area of DEHP-treated chick embryos (Fig. ...
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... shown in Fig. 3, ROS level was significantly restrained in DEHP-treated chick embryos by CHO (100 μg/μl), especially in heart and brain (Fig. 3, B2 and B3; Table 3). CHO (100 μg/μl) supplement significantly increased total SOD (Fig. 4A, P < 0.05; Table 3) and Cu-Zn SOD activity in DEHP-treated chick embryos ( Fig. 4A, P < 0.01; Table 3). However, CHO (100 μg/μl) supplement had no obvious effects on MDA content and GPX activity in DEHPtreated chick embryos. Other nutrients, such as Fe ...
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... effect of DEHP on the development of yolk sac vascellum was analyzed by vascular area (Fig. 2). The vascular area was significantly decreased in DEHP-treated chick embryos, compared with control ( Fig. 2, P < 0.01), implying that yolk sac vascellum development was injured by DEHP exposure. ...
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... effect of DEHP on the development of yolk sac vascellum was analyzed by vascular area (Fig. 2). The vascular area was significantly decreased in DEHP-treated chick embryos, compared with control ( Fig. 2, P < 0.01), implying that yolk sac vascellum development was injured by DEHP exposure. ...
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... that the fluorescence signals of ROS was increased 2.8 fold after DEHP treatment in chick embryos compared with the control (Fig. 3), implying that DEHP could lead to the production of oxidative stress in chick embryos. The result was similar from the analysis of fluorescence signals of ROS in brain, heart and spine of chick embryos, respectively (Fig. 3 B2−B4). Oxidative stress indicators, including SOD, MDA and GPX, were also altered following DEHP treatment. The total SOD activity was dramatically reduced in chick embryos treated by DEHP (P < 0.05), as compared with the control (Fig. 4). Cu-Zn SOD activity was also obviously decreased when DEHP (P < 0.01) was administered to chick embryos. ...
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... and TUNEL assay. As shown in Fig. 5, TUNEL-positive signals were obviously elevated in whole embryo after DEHP treatment, compared with the control (P < 0.01; Fig. 5), which implied that DEHP may affect apoptosis. The result was similar from the analysis of TUNEL-positive signals in brain, heart and spine of chick embryos, respectively (Fig. 5 ...
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... reverse effect on the embryo survival, embryo body weight and embryo malformation in DEHP-treated chick embryos (Table 3). CHO (100 μg/μl) could partially reverse these effects (Table 3). Moreover, the vascular area of yolk sac vascellum was obviously increased in DEHP-treated chick embryos by CHO (100 μg/μl) supplement compared with control ( Fig. 2, P < 0.01). However, no remarkable reverse effect was observed with the supplement of Fe (20%) and FA (10 μg/μl) on the vascular area of DEHP-treated chick embryos (Fig. ...
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... effects (Table 3). Moreover, the vascular area of yolk sac vascellum was obviously increased in DEHP-treated chick embryos by CHO (100 μg/μl) supplement compared with control ( Fig. 2, P < 0.01). However, no remarkable reverse effect was observed with the supplement of Fe (20%) and FA (10 μg/μl) on the vascular area of DEHP-treated chick embryos (Fig. ...
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... shown in Fig. 3, ROS level was significantly restrained in DEHP-treated chick embryos by CHO (100 μg/μl), especially in heart and brain (Fig. 3, B2 and B3; Table 3). CHO (100 μg/μl) supplement significantly increased total SOD (Fig. 4A, P < 0.05; Table 3) and Cu-Zn SOD activity in DEHP-treated chick embryos ( Fig. 4A, P < 0.01; Table 3). However, CHO (100 μg/μl) supplement had no obvious effects on MDA content and GPX activity in DEHPtreated chick embryos. Other nutrients, such as Fe ...
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