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Effects of Biota orientalis L. leaf extract (BOLE), hair growth plant ethanol extract (HGPEE), hair growth plant ethanol extract (HGPEE), and minoxidil on the number of hamster sebocytes (a); amount of lipids per cell (b); and size of lipid droplets (c). n = 3, mean ± S.D. ## p < 0.01 vs. Control (Undifferentiated); * p < 0.05 vs. Control (Differentiation); ** p < 0.01 vs. Control (Differentiation); + p < 0.1 vs. Control (Differentiation).
Source publication
Biota orientalis L. leaf extract (BOLE) is used medically to improve strength and arrest hemorrhage. In China, BOLE has been used in traditional medicine for its antibacterial properties and for hair restoration. In this study, we investigated the mechanism of hair restoration by BOLE from the point of view of the sebum suppressant effect and hair...
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Context 1
... investigated the mechanism of hair restoration by BOLE on the basis of the sebum suppressant effect. Cell proliferation in the differentiation culture medium was significantly inhibited to three-fifths that of the non-differentiation culture medium (Figure 1). The amount of lipids per cell increased significantly by 2.3-fold and the intracellular lipid droplets also increased significantly by 7.4-fold (Figure 1). ...
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... proliferation in the differentiation culture medium was significantly inhibited to three-fifths that of the non-differentiation culture medium (Figure 1). The amount of lipids per cell increased significantly by 2.3-fold and the intracellular lipid droplets also increased significantly by 7.4-fold (Figure 1). There were no significant differences in cell proliferation among the control and BOLE (25, 50 µg/mL), HGPEE (50 µg/mL), and HGPWE (50 µg/mL) in the differentiation culture medium; however, cell proliferation was promoted by minoxidil (20 µg/mL) relative to that of the control (Figure 1). ...
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... amount of lipids per cell increased significantly by 2.3-fold and the intracellular lipid droplets also increased significantly by 7.4-fold (Figure 1). There were no significant differences in cell proliferation among the control and BOLE (25, 50 µg/mL), HGPEE (50 µg/mL), and HGPWE (50 µg/mL) in the differentiation culture medium; however, cell proliferation was promoted by minoxidil (20 µg/mL) relative to that of the control (Figure 1). ...
Context 4
... size of the intracellular lipid droplet became significantly small after treatment with BOLE (25, 50 µg/mL) and HGPEE (50 µg/mL) relative to the size after treatment with the control, and the size tended to decrease after treatment with HGPWE (50 µg/mL) in the differentiated culture medium. No significant difference in size was seen between minoxidil (20 µg/mL) and the control in the differentiated culture medium (Figure 1). The shape of the sebocytes dyed with oil red O observed under microscope is shown in Figure 2. The cells were undifferentiated sebocytes, differentiated sebocytes, differentiated sebocytes + BOLE (50 μg/mL), differentiated sebocytes + HGPEE (50 μg/mL), differentiated sebocytes + HGPWE (50 μg/mL), and differentiated sebocytes + minoxidil (20 μg/mL). ...
Citations
... One of the most important application of plant preparations, including EOs and hydrolates, is on products for skin application, either as promotors of overall skin health or to treat different skin ailments. In fact, approximately one-third of all traditional medicines that include different plant preparations, are for treating wounds or skin disorders (Zeng et al., 2017). This gains major relevance considering that skin diseases are the fourth most common cause of all human diseases and affect almost one-third of the world's population, with their burden being underestimated, despite their visibility (Flohr and Hay, 2021). ...
Ethnopharmacological relevance:
Thymus mastichina (L.) L. (TM) and Cistus ladanifer L. (CL) are two Portuguese autochthonous species with traditional skin application in folk medicine. TM is majorly known for its antiseptic and wound healing properties, as an external anti-inflammatory agent and for its application in folk cosmetics and hygiene products. Its use in acne vulgaris has also been reported. CL is traditionally used in remedies for wounds, ulcers and other skin ailments such as psoriasis and eczema. Its application has been found useful due to its anti-inflammatory, astringent, wound healing and antiseptic properties.
Aim of the study:
With this work, we aimed to investigate relevant bioactivities related with the traditional application of TM and CL essential oils (EOs) and hydrolates (by-products of EO production) in skin ailments. Specifically their in vitro antioxidant, anti-inflammatory, cytotoxic, wound healing and antimicrobial properties were evaluated. The chemical composition of both EOs and respective hydrolates was also characterized.
Materials and methods:
Chemical characterization of EOs and hydrolates was performed by GC-FID and GC-MS. Cellular biocompatibility was evaluated using the MTT assay in macrophages (RAW 264.7) and fibroblasts (L929) cell lines. Anti-inflammatory activity was investigated by studying nitric oxide (NO) production by macrophages with griess reagent. Wound healing potential was evaluated with the scratch-wound assay. The antioxidant potential was studied by the DPPH scavenging method. Antimicrobial activity was evaluated by broth microdilution assay against relevant microbial strains and skin pathogens, namely Staphylococcus aureus, Staphylococcus epidermidis, Cutibacterium acnes, Pseudomonas aeruginosa, Escherichia coli, Candida albicans and Aspergillus brasiliensis.
Results:
The major compounds present in TM and CL EOs were 1,8-cineole and α-pinene, respectively. 1,8-cineole and E-pinocarveol were the major compounds in the correspondent hydrolates. CL EO presented the highest anti-inflammatory potential (EC50 = 0.002% v/v), still with significant cytotoxicity (IC50 = 0.012% v/v). TM preparations presented anti-inflammatory potential, also presenting higher biocompatibility. The same profile was present on fibroblasts regarding biocompatibility of the tested preparations. CL EO and hydrolate increased fibroblasts' migration by 155.7% and 148.4%, respectively. TM hydrolate presented a milder activity than CL hydrolate, but wound healing potential was still present, increasing cell migration by 125.1%. All preparations presented poor antioxidant capacity. CL EO presented higher antimicrobial activity, with MICs ranging from 0.06% v/v to 2% v/v, against different microorganisms.
Conclusions:
Anti-inflammatory and skin repairing potential were present for CL preparations. TM hydrolate presented an interesting biocompatible profile on both cell lines, also presenting anti-inflammatory potential. Furthermore, EOs from both species presented antimicrobial activity against a panel of different microorganisms. These in vitro bioactivities support some of their traditional skin applications, specifically regarding their antiseptic, wound healing and anti-inflammatory uses.
... Furthermore, clinical studies and reports revealed a wide range of pharmacological properties and medicinal applications, namely anticancer, neuro-protective, antimicrobial, antiviral, antioxidant, and antiinflammatory (Emami et al., 2005;Shan et al., 2014). Indeed, P. orientalis leaves and needles extracts have been reported to have diuretic, antioxidant, antidiabetic, fungitoxic, antibacterial and neuroprotective effects (Nizam and Mushfiq, 2007;Guleria et al., 2008;Pearson et al., 2009;Jasuja et al., 2013;Mohadjerani et al., 2016;Seo et al., 2017) and could even be used in cosmetics (Zeng et al., 2017). Phytochemistry analyses of P. orientalis shed the light on many chemical constituents such as diterpenes and flavonoids from essentials oils ( Lee et al., 2009;Ismail et al., 2013;Kim et al., 2013;Shan et al., 2014) or fatty acids from seeds oil (Lie et al., 1991). ...
... Etanolowy ekstrakt z rośliny wykazywał efekt pobudzający wzrost włosów i efekt odbudowy włosów, a także ograniczał ilość sebum. Wnioskiem z tego badania może być fakt, iż odżywki do włosów zawierające etanolowy wyciąg z liści Biota orientalis mogą być przydatne do stymulacji wzrostu włosów i zmniejszania nadmiaru sebum skóry głowy u mężczyzn i kobiet [27]. ...
Seborrhea is a serious dermatological problem which concerns disturbances in the activity of the skin sebaceous glands. It is based on the microbiota changes with fungal disorders. An important role in seborrhea play genetic factors, hormonal diseases, improper diet, deficiencies of certain vitamins and external factors, such as inadequate care treatments. The aim of the study was to analyze plant materials exhibiting a high anti-seborrhoeic potential and to review current studies of selected plant extracts with anti-seborrheic activity and their possible therapeutic effects. In addition to personalized therapy that allows the normalization of the work of the sebaceous glands, cosmetic treatments and topical application of appropriate medicinal plant-based products are also important. Acquiring and planning a well-chosen composition of highly effective plant extracts allows to create a natural cosmetic that safely reduce seborrhea without any additional side effects.
... [12][13][14][15][16] Active components of herbs are widely used in cosmetics and medicines to improve the beneficial effect of the final products; the popularity of the natural, herbal-based cosmetics is increasing steadily. [17][18][19][20][21][22] Most recently, not only the nature of the active components but also the method of administration plays a crucial role in drug preparations to optimize their positive effects. Several types of materials have been used as drug carriers and their improvement is an important research area. ...
... The Journal of Supercritical Fluids 148 (2019) [16][17][18][19][20][21][22][23] which may be indicated by the insignificant change in the mesopore contribution (88% vs. 89%). The shrinking most likely happened in the higher macropore region, which cannot be measured by this technique. ...
... The C constant of the pristine hydrophobic aerogel was 27.1, indicating an apolar, hydrophobic surface. The value dropped to 23.2 on adsorption, meaning that the adsorbed [16][17][18][19][20][21][22][23] materials made the surface even more hydrophobic. ...
Static supercritical fluid extraction has been used to concentrate the aroma materials of common herbs and spices. The technique has provided a higher number of components and cleaner extract than the one-step ethanol maceration. The one-step supercritical fluid extraction of the aroma compounds has been combined with their in situ adsorption in hydrophilic and hydrophobic silica aerogels. The extracts have been analyzed by a GC–MS technique and 55 aroma compounds have been identified. Most of the compounds have been adsorbed in both polar and apolar silica aerogels with no direct connection with the surface polarity. However, previously undetected compounds enriched to an analytically significant level, while others competed with each other for the active sites on the surface. Functionalized silica aerogels can be used as a new type of aroma storage materials and as selective and tuneable adsorbents for the extraction and enrichment of potentially active components from a complex matrix.
This narrative review aims to examine the therapeutic potential and mechanism of action of plant extracts in preventing and treating alopecia (baldness). We searched and selected research papers on plant extracts related to hair loss, hair growth, or hair regrowth, and comprehensively compared the therapeutic efficacies, phytochemical components, and modulatory targets of plant extracts. These studies showed that various plant extracts increased the survival and proliferation of dermal papilla cells in vitro, enhanced cell proliferation and hair growth in hair follicles ex vivo, and promoted hair growth or regrowth in animal models in vivo. The hair growth-promoting efficacy of several plant extracts was verified in clinical trials. Some phenolic compounds, terpenes and terpenoids, sulfur-containing compounds, and fatty acids were identified as active compounds contained in plant extracts. The pharmacological effects of plant extracts and their active compounds were associated with the promotion of cell survival, cell proliferation, or cell cycle progression, and the upregulation of several growth factors, such as IGF-1, VEGF, HGF, and KGF (FGF-7), leading to the induction and extension of the anagen phase in the hair cycle. Those effects were also associated with the alleviation of oxidative stress, inflammatory response, cellular senescence, or apoptosis, and the downregulation of male hormones and their receptors, preventing the entry into the telogen phase in the hair cycle. Several active plant extracts and phytochemicals stimulated the signaling pathways mediated by protein kinase B (PKB, also called AKT), extracellular signal-regulated kinases (ERK), Wingless and Int-1 (WNT), or sonic hedgehog (SHH), while suppressing other cell signaling pathways mediated by transforming growth factor (TGF)-β or bone morphogenetic protein (BMP). Thus, well-selected plant extracts and their active compounds can have beneficial effects on hair health. It is proposed that the discovery of phytochemicals targeting the aforementioned cellular events and cell signaling pathways will facilitate the development of new targeted therapies for alopecia.
Macromolecules are large, complex molecules composed of smaller subunits known as monomers. The four primary categories of macromolecules found in living organisms are carbohydrates, lipids, proteins, and nucleic acids; they also encompass a broad range of natural and synthetic polymers. Recent studies have shown that biologically active macromolecules can help regenerate hair, providing a potential solution for current hair regeneration therapies. This review examines the latest developments in the use of macromolecules for the treatment of hair loss. The fundamental principles of hair follicle (HF) morphogenesis, hair shaft (HS) development, hair cycle regulation, and alopecia have been introduced. Microneedle (MN) and nanoparticle (NP) delivery systems are innovative treatments for hair loss. Additionally, the application of macromolecule-based tissue-engineered scaffolds for the in vitro and in vivo neogenesis of HFs is discussed. Furthermore, we explored a new research direction wherein artificial skin platforms are adopted as a promising screening method for hair loss treatment drugs. Through these multifaceted approaches, we identified promising aspects of macromolecules for future hair loss treatments. This article is protected by copyright. All rights reserved.
Hair disorders such as hair loss (alopecia) and androgen dependent, excessive hair growth (hirsutism, hypertrichosis) may impact the social and psychological well‐being of an individual. Recent advances in understanding the biology of hair have accelerated the research and development of novel therapeutic and cosmetic hair growth agents. Preclinical models aid in dermocosmetic efficacy testing and claim substantiation of hair growth modulators. The in vitro models to investigate hair growth utilize the hair follicle Dermal Papilla cells (DPCs), specialized mesenchymal cells located at the base of hair follicle that play essential roles in hair follicular morphogenesis and postnatal hair growth cycles. In this review, we have compiled and discussed the extensively reported literature citing DPCs as in vitro model to study hair growth promoting and inhibitory effects. A variety of agents such as herbal and natural extracts, growth factors and cytokines, platelet‐rich plasma, placental extract, stem cells and conditioned medium, peptides, hormones, lipid‐nanocarrier, light, electrical and electromagnetic field stimulation, androgens and their analogs, stress‐serum and chemotherapeutic agents etc. have been examined for their hair growth modulating effects in DPCs. Effects on DPCs’ activity were determined from untreated (basal) or stress induced levels. Cell proliferation, apoptosis and secretion of growth factors were included as primary end‐point markers. Effects on a wide range of biomolecules and mechanistic pathways that play key role in the biology of hair growth were also investigated. This consolidated and comprehensive review summarizes the up‐to‐date information and understanding regarding DPCs based screening models for hair growth and may be helpful for researchers to select the appropriate assay system and biomarkers. This review highlights the pivotal role of DPCs in the forefront of hair research as screening platforms by providing insights into mechanistic action at cellular level, which may further direct the development of novel hair growth modulators. This article is protected by copyright. All rights reserved.
