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Different wheat varieties namely DWR 16, PBW 550, DWR 39, DBW 17, DPW 621-50, DBW 14 and HD 2967 were evaluated for spawn production of button mushroom (Agaricus bisporus strain U-3). DPW 621-50 resulted in maximum downward linear growth followed by DBW 17 and HD 2967, respectively. Analysis of nutritional profile of different varieties revealed th...
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... to unfavourable microclimate and ultimately reduces the growth. Whereas excessive washing may remove the suspended gluten and other nutrients from the surroundings of boiled wheat grains resulting in comparatively less growth. Supplementation of wheat grains with calcium carbonate and calcium sulphate favoured rapid growth of A.bisporus mycelium (Fig. 2) as calcium is an essential nutrient for the mushroom mycelium (Lambert, 1950). It was further observed that, before inoculation of commercial spawn, disintegration of fully colonised grains of master spawn was easier in case of washed grains than in unwashed ...
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... Past studies indicates that, beside the correctly compost substrate, the oyster mushroom does well under certain levels of temperature, humidity, light and ventilation, while the quality of spawn is affected by the mother culture [5], media culture concentrations and spawn substrate preparations [6]. Other several studies indicate that oyster mushrooms yield depends on the type of substrate used and the quality of the spawns [7] [8]. ...
... The calcium sulphate prevents the grains from sticking and calcium carbonate is used to adjust the pH (Stoller, 1962). The best results have been obtained by using 2% calcium sulphate and 0.5% calcium carbonate on dry weight basis of grains but different researchers used different ratios for mixing them (Sharma et al., 2013;Dehariya et al., 2011;Kumari and Achal, 2008;Ali et al., 2007) The present study was to compare the characteristics and suitability of different cereal grains as single substrate for the production of spawns of button mushroom following their different hydration treatment to make approximately 50% moisture content. Calcium sulphate and calcium carbonate were not applied to understand the basic characteristics of grain materials. ...
... For this purpose usually 2% calcium sulphate and 0.5% calcium carbonate on dry weight basis of grains were tried. However, different researchers used different ratios for mixing them (Dehariya et al., 2011;Kumari and Achal, 2008;Ali et al., 2007) and they seem to be not essential for the mycelial growth (Sharma et al., 2013). The pH level of wheat grain was 6.5 without supplementation of calcium sulphate and calcium carbonate. ...
... Although it may be required in lower concentration, other elements such as sulphur, magnesium, calcium and iron play an essential role and should be included in the media (Wood and Fermor, 1985). In other researches, however, mineral elements such as zinc, copper, iron, manganese were required in very minute quantities and did not show direct relationship with mycelial growth in button mushroom (Hayes, 1972;Sharma et al., 2013). ...
Spawn is the vegetative growth of the mycelium and serves as the inoculum or seed for cultivating mushrooms. Various cereal grains, such as wheat, millet, barley, sorghum, brown rice, rye, and oat were assessed to compare their characteristics and suitability for spawn production of button mushroom (Agaricus bisporus). The mycelial growth rates, density, and the number of completely colonized grains were measured from the twentieth day of inoculation. Wheat grains showed fastest mycelial growth with 8.4 cm followed by rye, oat, barley with 8.2, 7.5 and 7.3 cm, respectively. In the mycelial density, foxtail millet, barley, and sorghum were best compared with that of wheat grains. Especially, the number of grains which were completely colonized by mycelia were greatest in foxtail millet with 5,123 grains followed by proso millet, and wheat with 3,052 and 914, respectively. Based on the results obtained, barley, foxtail millet, and sorghum grains would be appropriate substituting for wheat grain in spawn production of button mushroom.
