Fig 4 - uploaded by Shanthi Mookiah
Content may be subject to copyright.
Effect of phylloplane antagonists against B. oryzae in vitro Biochemical characterization of Pseudomonas spp. isolates All the ten isolates of Pseudomonas spp. gave positive reaction to fluorescent pigment production, KOH test, growth at 4 °C, growth at 45 °C, catalase, citrate utilization, nitrate
Source publication
The brown spot of rice is a devastating disease in rice production. It is caused by the pathogen Bipolaris oryzae which reduces many quality and quantity impacts on the rice crop. It has been known well that some phylloplane bacterial antagonists are antagonistic to some foliar diseases. The research work was carried out with the aim to study the a...
Citations
... After incubation single bacterial colonies were streaked on the slants containing Nutrient Agar. These agar slants were used for the further studies (Rajasekar et al. 2019). ...
... Phylloplane bacterial isolates PB2, PB4, PB5, PB8, PB10 Produced positive result in gram staining, starch hydrolysis, gelatin liquefaction, casein hydrolysis, IAA production test, HCN production test, Catalase test, glucose utilization, sucrose utilization. Negative results were obtained C-T C I= Thalhun et al. (2017), Prabhukarthikeyan et al. (2015) , Kumar et al. (2014), Rajashekhar et al. (2017)and Rajasekar et al. (2019) also reported similar such results in their respective experiments. ...
... fluorescens) (Table 3). Similarly, Rajasekar et al. (2019) reported the antifungal activity of Rice Associated Phyllosphere (RAP) communities with twenty phylloplane bacterial isolates tested against B. oryzae. Among the twenty isolates B. subtilis (PI5) showed the maximum inhibition (52.96%) followed by P. fluorescens (PI1) with 52.59 % inhibition over control. ...
Background: Rice brown leaf spot is a major fungal disease caused by Bipolaris oryzae. Biological management of brown leaf spot can be achieved by bacteria associated with phyllosphere region of rice crop. Methods: Antagonistic bacteria were isolated from phylloplane of the rice crop using dilution method. Phylloplane bacteria were morphologically and biochemically characterized. Antagonistic activity of bacteria was tested against Bipolaris oryzae using Dual culture technique. Result: Among the isolates, PB1, PB3, PB6, PB7, PB9 were identified as Pseudomonas fluorescens and PB2, PB4, PB5, PB8, PB10 were identified as Bacillus subtilis by morphological and biochemical characterization. Phylloplane bacteria PB5 showed the maximum inhibition of B. oryzae mycelium (70.00%) followed by PB1 (67.77%). Minimum inhibition of mycelial growth (31.11) was found in PB9.
