Fig 8 - uploaded by Lorenzo Zacarías
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Effect of ethylene on the expression of the (A) ethyleneresponsive factor 1 and (B) 2 (ERF1, ERF2) in the flavedo of MG 'Comune' and 'Tardivo' mandarin fruit (Citrus clementina). White and black bars represent air and ethylene-treated fruits, respectively. The data are means ± SE of at least three replicates.
Source publication
Tardivo' mandarin is a mutant of 'Comune' Clementine with a delay in peel degreening and coloration, allowing late harvesting. In this work, we have explored if the late-harvesting phenotype of 'Tardivo' mandarin is related to altered perception and sensitivity to ethylene. The peel degreening rate was examined after a single ethephon treatment or...
Contexts in source publication
Context 1
... inability of 'Tardivo' fruits to maintain ethylene- induced gene expression could be also related to altered downstream element in the signal transduction pathway, as ERF transcription factors that have been shown to be essential modulators of fruit ripening ( Gapper et al. 2013). Analysis of the expression of two ERFs, ERF1 and ERF2, homologous to genes previously isolated in other Citrus species (Citrus unshiu) ( Yang et al. 2010), revealed that these genes are negatively regulated by ethylene in the peel of 'Comune' mandarin (Fig. 8). These pattern of expression suggested that such ERFs may act in ordinary Citrus fruits as transcriptional repressors, similarly to that found in other plant systems (Fujimoto et al. 2000, Ohta et al. 2001, Chen et al. 2008). The response of ERF1 to the ethylene treatment in fruits of 'Tardivo' was similar to that of 'Comune' mandarin (Fig. 8A). However, the levels of ERF2 mRNA in 'Tardivo' fruits treated in air or ethylene were very similar (Fig. 8B). Although the pattern of expression of these genes did not correlate with the expected effect of ethylene and with the insensitivity of 'Tardivo' shown for other ethylene-regulated genes, results suggest an impairment in the expression of ERF1 and ERF2 in fruits of the mutant respect to the parental, in a way that the differences between air and ethylene-fruits were partially ...
Context 2
... inability of 'Tardivo' fruits to maintain ethylene- induced gene expression could be also related to altered downstream element in the signal transduction pathway, as ERF transcription factors that have been shown to be essential modulators of fruit ripening ( Gapper et al. 2013). Analysis of the expression of two ERFs, ERF1 and ERF2, homologous to genes previously isolated in other Citrus species (Citrus unshiu) ( Yang et al. 2010), revealed that these genes are negatively regulated by ethylene in the peel of 'Comune' mandarin (Fig. 8). These pattern of expression suggested that such ERFs may act in ordinary Citrus fruits as transcriptional repressors, similarly to that found in other plant systems (Fujimoto et al. 2000, Ohta et al. 2001, Chen et al. 2008). The response of ERF1 to the ethylene treatment in fruits of 'Tardivo' was similar to that of 'Comune' mandarin (Fig. 8A). However, the levels of ERF2 mRNA in 'Tardivo' fruits treated in air or ethylene were very similar (Fig. 8B). Although the pattern of expression of these genes did not correlate with the expected effect of ethylene and with the insensitivity of 'Tardivo' shown for other ethylene-regulated genes, results suggest an impairment in the expression of ERF1 and ERF2 in fruits of the mutant respect to the parental, in a way that the differences between air and ethylene-fruits were partially ...
Context 3
... inability of 'Tardivo' fruits to maintain ethylene- induced gene expression could be also related to altered downstream element in the signal transduction pathway, as ERF transcription factors that have been shown to be essential modulators of fruit ripening ( Gapper et al. 2013). Analysis of the expression of two ERFs, ERF1 and ERF2, homologous to genes previously isolated in other Citrus species (Citrus unshiu) ( Yang et al. 2010), revealed that these genes are negatively regulated by ethylene in the peel of 'Comune' mandarin (Fig. 8). These pattern of expression suggested that such ERFs may act in ordinary Citrus fruits as transcriptional repressors, similarly to that found in other plant systems (Fujimoto et al. 2000, Ohta et al. 2001, Chen et al. 2008). The response of ERF1 to the ethylene treatment in fruits of 'Tardivo' was similar to that of 'Comune' mandarin (Fig. 8A). However, the levels of ERF2 mRNA in 'Tardivo' fruits treated in air or ethylene were very similar (Fig. 8B). Although the pattern of expression of these genes did not correlate with the expected effect of ethylene and with the insensitivity of 'Tardivo' shown for other ethylene-regulated genes, results suggest an impairment in the expression of ERF1 and ERF2 in fruits of the mutant respect to the parental, in a way that the differences between air and ethylene-fruits were partially ...
Context 4
... pattern of expression of ERF1 and ERF2 was differ- ent and there were also differences between genotypes. While in 'Comune' fruits ERF1 expression levels slightly decreased on day 2 and increased thereafter, ERF2 mRNA abundance increased progressively during incu- bation in air. In fruits of 'Tardivo' the expression was the opposite, since ERF1 mRNA levels slightly increased and ERF2 transcript abundance showed a moderate decrease (Fig. 8). Interestingly, ethylene suppressed the induction of ERF1 and ERF2 genes in the peel of 'Comune' fruits. However, the effect of ethylene was more moderated in fruits of 'Tardivo', and after 5 days of treatment the levels of both ERFs were higher than in the corresponding tissue of 'Comune' (Fig. ...
Context 5
... pattern of expression of ERF1 and ERF2 was differ- ent and there were also differences between genotypes. While in 'Comune' fruits ERF1 expression levels slightly decreased on day 2 and increased thereafter, ERF2 mRNA abundance increased progressively during incu- bation in air. In fruits of 'Tardivo' the expression was the opposite, since ERF1 mRNA levels slightly increased and ERF2 transcript abundance showed a moderate decrease (Fig. 8). Interestingly, ethylene suppressed the induction of ERF1 and ERF2 genes in the peel of 'Comune' fruits. However, the effect of ethylene was more moderated in fruits of 'Tardivo', and after 5 days of treatment the levels of both ERFs were higher than in the corresponding tissue of 'Comune' (Fig. ...
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