Table 1 - uploaded by Adel Mahmoud
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EST forward and reverse primer pairs sequences.

EST forward and reverse primer pairs sequences.

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Article
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Conventional wheat breeding programs aim at developing high yielding cultivars suitable for sowing under stress conditions and poor soils especially in newly reclaimed deserts. The objective of the current study was to develop high yielding and early maturing bread wheat (Triticum aestivum L.) lines adapted to grow in old and new reclaimed desert s...

Contexts in source publication

Context 1
... the basis of sequences of four wheat EST markers, PCR primer pairs were designed and used to screen the polymorphism among the studied lines. The primer sequences are shown in Table 1. PCR was carried out under the following conditions; one initial strand separation cycle at 94°C for 4 min followed by 32 cycles including a denaturation step at 94°C for 30 s, an annealing step at (50 to 60°C, depending on the primer sequence) for 30 s and polymerization step at 72°C for 1 min, with final extension step at 72°C for 5 min. ...
Context 2
... lines Assiut-1, Assiut-2 and Assiut-3 gave grain yield significantly higher than that of the check variety. On the other hand, two inbred lines (that is Line 95 and Line 92) gave grain yield lower than that of the check variety at all the three As st-1 Asst-2 Asst-3 Line 95 Line 92 Selected Lines locations (Table 4 and Figure 8). Figure 8 showed that inbred line Assiut-1 gave the highest grain yield (8,356 kg/ha) averaged over environments and line Assiut-2 occupied the second place for grain yield (8250 kg/ha). ...

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Citations

... Together, they import more grain than all of South America (Stecker 2011). This is because most land in Egypt are deserts ( Khieralla et al. 2011). Newly reclaimed soils in these deserts suffer from various stresses such as drought, salinity, nutrient deficiency, etc. ( El-Fouly et al. 1984). ...
... Newly reclaimed soils in these deserts suffer from various stresses such as drought, salinity, nutrient deficiency, etc. ( El-Fouly et al. 1984). On the other hand, population increase in Egypt is taking place at an alarming rate of 2.52-8%/annum ( Khieralla et al. 2011). This tremendous increase in population is one of the most serious challenges the country now faces. ...
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The climate is changing all over the world, particularly in semi-arid and arid regions. This changing climate could strongly affect wheat production worldwide. As the world population continues to grow, and water resources for crop production decline and temperature increases, so the development of heat- and drought-tolerant cultivars is an issue of global concern. In this context, two cycles of selection were employed in the F2 generation of a cross between bread wheat (Triticum aestivum L.) ‘Long spike 58’ and ‘Giza-168’ to assess the impact of selection on grain yield in response to drought. Family selection and within-family selection were adopted in the second cycle of selection. The observed response to selection for grain yield was 64.66% in the F3 generation and 18.14 and 12.39% in the F4 generation for family and within-family selection, respectively. The mean grain yield of F3 selections exceeded that of two standard cultivars (‘Giza-168’ and ‘Sids 12’) by 19.67 and 16.48%, respectively, while the mean of F4 selections exceeded that of the two standard cultivars by 15.08 and 35.53%, respectively. Significant positive correlations were obtained for 1000-grain weight (32.34), number of kernels spike-1 (24.8) and spike length (12.55) in the F3 generation but not in the second cycle of selection. Generally, the observed responses to selection were greater than the predicted responses indicating the presence of dominant gene effects for the traits studied.
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