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Dose and duration dependent variations in the specific activities of few lysosomal enzymes from the intestine tissue of mice after in vitro exposure of Aroclor 1254.
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Polychlorinated biphenyls (PCBs) are industrial POPs which have been released into the environment resulting in widespread and persistent contamination. The aim of the present investigation was to determine the alterations in the activities of few lysosomal enzymes (acid phosphatase, α-galactosidase, β-galactosidase and β-glucuronidase) in lysosoma...
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In surficial sediments of Lake Piediluco, a small, riverine, regulated and heavily modified water body in the Central Italian Apennines, contamination by persistent organochlorine pollutants (POPs) was assessed. During spring and autumn, six representative points were investigated. Reflecting a substantial zonation of pollution within this aquatic...
Aroclor 1254 contains very high concentration of chorine which makes it a persistent and toxic pollutant in the environment. The references of its toxic effects on liver and brain lysosomal biomarker enzymes are not abundantly available. Present study reports the possible dose and duration dependent toxic effects of low dose of Aroclor 1254 on few...
Citations
... They provide cell energy production by coupling the chemical reaction in living organism (Takao, 1985). ATPases are present in all the cell membranes and regulates the ionic concentrations in the cell (Kundu and Patel, 2005;Bhuva et al., 2014). ATPases transports the ions to regulate the cellular volume, osmotic pressure, and membrane permeability through biological membranes (Li et al., 2011). ...
This communication reports the dose and duration dependent Cr (VI) toxicity on the activity of four membrane bound ion dependent ATPase (Total, Na+- K+, Ca2+ and Mg2+ATPases) in mice kidney. Groups of mice were exposed to different sublethal doses (5, 10, 15 and 20 mg/kg bw/d) of Cr (VI) for four exposure durations (7, 14, 21 and 28 days). The administration of toxicant was through oral route. Results indicated significant dose and duration dependent effects of Cr (VI) on the activities of all membrane bound ATPases studied in the mice kidney tissue. Results suggested a greater exposure duration dependent effects in all doses compared to the control. The enzyme activity was inhibited in the shorter durations but stimulated after longer exposure durations. The results
suggested that the alterations in the activity of ATPases possibly affecting the movement of ions across the cell membrane, creating osmotic imbalance inside the cell which ultimately lead the disturbance in the normal physiological activity of kidney tissue.
... The overall results of the present study showed that these alterations in the activities of selected membrane-bound iondependent ATPases and lysosomal enzymes of mice kidney may create the cellular disturbances by affecting the transport of ions and metabolic pathways which leads to alterations in the normal cellular physiological processes (Bhuva et al., 2014). The results of the present study answer the hypothesis that the exposure of very low and environmentally available doses of TCDD has a direct dose dependent effects on the activities of selected ATPases and lysosomal enzymes under in vitro condition. ...
A dioxin, the 2,3,7,8-TCDD is a highly toxic POP, produced during the improper burning of paper, pulp,
municipal and industrial wastes, and tend to accumulate in the adipose tissues of animals including human when exposed through different environmental sources. However, its toxicity reports on the animal organ systems in general and kidney lysosomal enzymes in particular are rare. The present communication therefore, tested the hypothesis that relatively low and environmentally available doses of TCDD affects the activities of few membranebound ion-dependent ATPases (Total, Na+-K+ and Ca2+ ATPases) and lysosomal marker enzymes which triggers the cellular apoptosis process (acid phosphatase, â-glucuronidase and á-galactosidase) of mice kidney under in vitro conditions. The results suggested a significant direct dose dependent effects of TCDD on the selected ATPases and lysosomal enzymes extracted from mice kidney tissue. The results also indicating that even a very low dose of TCDD affects the activities of membrane bound ion dependent ATPases and lysosomal enzymes which may leads to severe disturbances in the trans-membrane movements of various ions and may initiate the cellular apoptosis process in the living system.
... In in vitro conditions, Aroclor 1254 comes in direct contact with the lysosomal enzymes and inhibit the activities while under in vivo condition, the toxicant is possibly comes into contact with the lysosomal enzymes via indirect ways. It is possible that the PCB passes through the cellular metabolic pathways and produces the hazardous metabolites which come into contact with these enzymes and induce the observed effects in the enzyme activity [7,25]. The overall results of the present study revealed that these alterations in the activities of lysosomal enzymes of mice liver and brain may create the cellular disturbances that affect the normal functional activities of lysosomes such as intracellular digestion and recycling of macromolecules [2]. ...
Aroclor 1254 contains very high concentration of chorine which makes it a persistent and toxic pollutant in the environment. The references of its toxic effects on liver and brain lysosomal biomarker enzymes are not abundantly available. Present study reports the possible dose and duration dependent toxic effects of low dose of Aroclor 1254 on few extracted lysosomal biomarker enzymes, acid phosphatase, α-galactosidase, β-galactosidase and β-glucuronidase from liver and brain tissues of mice under in vitro condition. Two hypotheses were tested in the present study, (a) Aroclor 1254 has a dose and duration dependent effects on the activities of selected extracted enzymes and (b) the response exhibits an organ specific effect of the toxicant. The results revealed both significant exposure duration dependent and organ specific effects on the activities of lysosomal enzymes. The observed results indicated that if Aroclor 1254 enters into the cell and comes in direct contact with the lysosomal enzymes, it may influence the activities of the lysosome in a complex, organ specific way. Though the exact nature of the influences at cellular level is not clear, these may lead to an increase in the cellular accumulation of toxic substances which may contribute adverse toxic effects on the gross physiological conditions of the cell and the organism.
Present study was conducted to understand the in vivo dose and exposure duration
dependent hazardous effects of low doses Aroclor 1254, a highly toxic POP, on few lysosomal enzymes, like acid phosphadase, alfa galactosidase, beta galactosidase and beta glucoronidase, in the liver tissue of Swiss Albino mice. The study tests the hypothesis that in vivo exposure of low dose Aroclor 1254 provokes dose and duration dependent toxic effects to lysosomal enzymes and thereby causes changes in the cellular apoptosis process. Total no. of 24 adult male Swiss albino mice (30±40 g) were taken and divided into one control and two dose groups (0.1 and 1 mg/kg/day) and four different exposure duration groups (7, 14, 21 and 28 days). Results revealed activities of the selected lysosomal enzymes after the exposure of both the doses of Aroclor 1254 for 7 and 14 days, the activities of lysosomal enzymes showed inhibition while, in higher exposure durations (21 and 28 days), the enzymes activities showed stimulation as
compared to control. Therefore, the results suggested that Aroclor 1254 has the capacity to inhibit the activity of lysosomal enzymes which may cause disturbances in the intracellular digestion and recycling of macromolecules that may lead to disturbances in the immune system and overall health of organisms under in vivo conditions.
Present study was conducted to understand the in vivo dose and exposure duration
dependent hazardous effects of low doses Aroclor 1254, a highly toxic POP, on few lysosomal enzymes, like - - -glucuronidase, in the liver tissue of Swiss Albino mice. The study tests the hypothesis that in vivo exposure of low dose Aroclor 1254 provokes dose and duration dependent toxic effects to lysosomal enzymes and thereby causes changes in the cellular apoptosis process. Total no. of 24 adult male Swiss albino mice (30±40 g) were taken and divided into one control and two dose groups (0.1 and 1 mg/kg/day) and four different exposure duration groups (7, 14, 21 and 28 days). Results revealed activities of the selected lysosomal enzymes after the exposure of both the doses of Aroclor 1254 for 7 and 14 days, the activities of lysosomal enzymes showed inhibition while, in higher exposure durations (21 and 28 days), the enzymes activities showed stimulation as compared to control. Therefore, the results suggested that Aroclor 1254 has the capacity to inhibit the activity of lysosomal enzymes which may cause disturbances in the intracellular digestion and recycling of macromolecules that may lead to disturbances in the immune system and overall health of organisms under in vivo condition.
Present communication reports the effects of environmentally available, low doses of tetra chloro di benzo-p-dioxin (2,3,7,8 TCDD) to lysosomal enzymes in mice liver. The study tests the hypothesis, in vivo exposure of low dose TCDD provokes dose and duration dependent toxic effects to key lysosomal enzymes and thereby causes cellular apoptotic changes. Three groups of female Swiss albino mice were subjected to two doses of TCDD (0.004 mg/kg bw/d, 0.04 mg/kg bw/d) for 2, 4 and 6 days of exposure durations. The results indicated significant exposure duration dependent effects of TCDD in mice liver cells. The results suggested that TCDD possibly induced an increase in intracellular ions or ROS which in turn altered different physiological activities by affecting different metabolic pathway of the liver cells. The altered functions of key lysosomal enzymes by TCDD may also evoke the process of cellular apoptosis.
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