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Dose and duration dependent variations in the specific activities of few lysosomal enzymes in the Brain tissue of mice after in vitro exposure of Aroclor 1254.
Source publication
Aroclor 1254 contains very high concentration of chorine which makes it a persistent and toxic pollutant in the environment. The references of its toxic effects on liver and brain lysosomal biomarker enzymes are not abundantly available. Present study reports the possible dose and duration dependent toxic effects of low dose of Aroclor 1254 on few...
Context in source publication
Context 1
... the other hand, the specific activity of β-galactosidase showed exposure duration dependent inhibitory trend after the exposure to different doses of Aroclor 1254. Similar results were observed in case of β-glucuorindase ( Figure 2). Polychlorinated biphenyls (PCBs) are highly lipophilic substances which have the ability to bioaccumulate in the body fats, including cellular membranes of animals and humans. ...
Citations
... Still these contaminants are found in the environment, which can cause the various serious physiological disturbances. These contaminants are of serious concern due to their persistence and toxicity at difference levels (Bhuva et al., 2014) Cells are associated with various subcellular organelles, which contains the variety of enzymes, transporters, channels, and receptors, and are potentially sensitive to toxicant related inhibition in their functions (Foulkes, 1998). Among the subcellular organelles, lysosomes play an important role in digestion, reproduction, cell repair and immune response as well as concentrate a wide range of toxic chemicals such as PAHs, PCBs, and metals from the cytoplasm (Moore, 1985). ...
... Among the subcellular organelles, lysosomes play an important role in digestion, reproduction, cell repair and immune response as well as concentrate a wide range of toxic chemicals such as PAHs, PCBs, and metals from the cytoplasm (Moore, 1985). Our previous studies showed that the alterations in the activities of some lysosomal enzymes after the short-term exposure of PCBs and TCDD, a dioxin like PCB (Awasthi et al., 2013;Jigyasi and Kundu, 2013;Raja et al., 2014). It has also been reported that the toxic chemicals induce the disturbances in the lysosomal membrane that can results an adverse effect on the activities of lysosomal enzymes and leads to cell death in many organisms like mussels, oysters, fish and earthworms (Kohlor et al., 1992;Lowe et al., 1995a;Ringwood et al., 1999). ...
... Lysosomal enzymes are known to be involved in cell death and tissue damage (Fushimi et al., 1974). Lysosomal enzymes play a potential role as indicators of metabolic compensations (Burlina et al., 1987), as the leaking of these enzymes into the cytosol causing cellular damages in different tissues (Maciejewski et al., 2001;Raja et al., 2014;Jigyasi et al., 2016). Several reports have demonstrated changes in lysosomal enzyme activities both in experimental animals and human (Belfiore et al., 1972;Hisako and Seiichiro, 1976;Gupta et al., 1999).The overall results of the present study revealed that these alterations in the activity of selected lysosomal enzymes may lead to the cellular processes disturbances (Storrie, 1995). ...
Present paper reports the dose and duration dependent effects of Aroclor 1254 on few antioxidative and lysosomal enzymes in the kidney tissue of male Swiss albino mice. Twenty four adult male Swiss albino mice (30±40 g) were exposed to two sublethal doses (0.1 and 1 mg/kg/day) for four exposure durations (7, 14, 21 and 28 days). The results indicated significant exposure duration dependent effects of Aroclor 1254 in mice kidney. Significant stimulation in the activities of antioxidant enzymes like glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD). On the other hand, significant exposure duration dependent inhibition was observed in the activity of GSH. However, a significant inhibition after 7 and 14 d exposure in both dose groups was evident in the activities of lysosomal enzymes, like acid phosphatase, á-galactosidase, â-galactosidase and â-glucuronidase. The alterations in these enzyme systems in the kidney tissue indicated adverse effects of lipophilic Aroclor 1254 in the kidney, which might be due to the direct as well as indirect effects of this lipid soluble POP which probably caused high level of cellular destructions.
Present study was conducted to understand the in vivo dose and exposure duration
dependent hazardous effects of low doses Aroclor 1254, a highly toxic POP, on few lysosomal enzymes, like acid phosphadase, alfa galactosidase, beta galactosidase and beta glucoronidase, in the liver tissue of Swiss Albino mice. The study tests the hypothesis that in vivo exposure of low dose Aroclor 1254 provokes dose and duration dependent toxic effects to lysosomal enzymes and thereby causes changes in the cellular apoptosis process. Total no. of 24 adult male Swiss albino mice (30±40 g) were taken and divided into one control and two dose groups (0.1 and 1 mg/kg/day) and four different exposure duration groups (7, 14, 21 and 28 days). Results revealed activities of the selected lysosomal enzymes after the exposure of both the doses of Aroclor 1254 for 7 and 14 days, the activities of lysosomal enzymes showed inhibition while, in higher exposure durations (21 and 28 days), the enzymes activities showed stimulation as
compared to control. Therefore, the results suggested that Aroclor 1254 has the capacity to inhibit the activity of lysosomal enzymes which may cause disturbances in the intracellular digestion and recycling of macromolecules that may lead to disturbances in the immune system and overall health of organisms under in vivo conditions.
Present study was conducted to understand the in vivo dose and exposure duration
dependent hazardous effects of low doses Aroclor 1254, a highly toxic POP, on few lysosomal enzymes, like - - -glucuronidase, in the liver tissue of Swiss Albino mice. The study tests the hypothesis that in vivo exposure of low dose Aroclor 1254 provokes dose and duration dependent toxic effects to lysosomal enzymes and thereby causes changes in the cellular apoptosis process. Total no. of 24 adult male Swiss albino mice (30±40 g) were taken and divided into one control and two dose groups (0.1 and 1 mg/kg/day) and four different exposure duration groups (7, 14, 21 and 28 days). Results revealed activities of the selected lysosomal enzymes after the exposure of both the doses of Aroclor 1254 for 7 and 14 days, the activities of lysosomal enzymes showed inhibition while, in higher exposure durations (21 and 28 days), the enzymes activities showed stimulation as compared to control. Therefore, the results suggested that Aroclor 1254 has the capacity to inhibit the activity of lysosomal enzymes which may cause disturbances in the intracellular digestion and recycling of macromolecules that may lead to disturbances in the immune system and overall health of organisms under in vivo condition.
This communication reports the dose and duration dependent Cr (VI) toxicity on the activity of four membrane bound ion dependent ATPase (Total, Na+- K+, Ca2+ and Mg2+ATPases) in mice kidney. Groups of mice were exposed to different sublethal doses (5, 10, 15 and 20 mg/kg bw/d) of Cr (VI) for four exposure durations (7, 14, 21 and 28 days). The administration of toxicant was through oral route. Results indicated significant dose and duration dependent effects of Cr (VI) on the activities of all membrane bound ATPases studied in the mice kidney tissue. Results suggested a greater exposure duration dependent effects in all doses compared to the control. The enzyme activity was inhibited in the shorter durations but stimulated after longer exposure durations. The results
suggested that the alterations in the activity of ATPases possibly affecting the movement of ions across the cell membrane, creating osmotic imbalance inside the cell which ultimately lead the disturbance in the normal physiological activity of kidney tissue.
