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In the present work three subspecies of Helichrysum plicatum DC. (Helichrysum plicatum DC. subsp. plicatum, Helichrysum plicatum DC. subsp. polyphillum (Ledeb) P.H.Davis & Kupicha and Helichrysum plicatum DC. subsp. isauricum Parolly) were investigated for the essential oil chemical compositions. The volatiles were obtained by conventional hydrodis...
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This study provided GC-FID and GC-MS analysis of the volatile constituents of Helichrysum italicum (Roth) G. Don, introduced from Corsica (France), cultivated in South Bulgaria, harvested in different phenological stages-fl oral budding period and fl owering period, and compared its quality to the quality of the species of native origin. Similariti...
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... Regarding the chemical profile of the members of the genus Helichrysum, the main compounds were pyrons, phloroglucinols, benzofurans, phthalides, terpenes, phenolic acids, flavonoids, chalcones, and essential oils (Eroglu et al., 2009;Akaberi et al., 2019;Öztürk et al., 2014;Bahşi, 2020). It has been claimed that phenolics, found in abundance in Helichrysum species, are powerful anti-inflammatory agents and could be used to treat skin conditions (Viegas et al., 2014). ...
... Humbert [11], H. picardii Boiss. & Reuter [12,13] [18], H. conglobatum (Viv.) Steudel. ...
... The inflorescence volatiles of H. plicatum subsp. isauricum were distinguished by predomination of monoterpene hydrocarbons (93.1%) with fenchene (88.3%) as the major constituent [18]. ...
The aerial parts of Helichrysum noeanum Boiss. and H. chionophilum Boiss. et Balansa were hydrodistilled for 3 h using a Clevenger-type apparatus. The essential oils were analyzed by GC-FID and GC-MS, simultaneously. The main constituents were identified as hexadecanoic acid (25.3%), dodecanoic acid (17.8%), tetradecanoic acid (13.7%), and decanoic acid (4.2%) for H. noeanum. Decanoic acid (18.7%), tetradecanoic acid (16.9%), dodecanoic acid (13.8%), hexadecanoic acid (12.2%) and hexahydrofarnesyl acetone (3.2%) were found to be the major compounds for H. chionophilum. Several reports have been encountered in the literature dealing with the oil composition of several Helichrysum species. To the best of our knowledge, the essential oil of H. chionophilum has not previously been investigated.
Helichrysum sanguineum (L.) Kostel (H. sanguineum), a member of the Asteraceae family, has been traditionally employed for various medicinal purposes owing to its rich phytochemical composition. This study investigates the anticancer properties of various extracts of H. sanguineum (ethanol, acetonitrile, hexane, and chloroform) against breast cancer cells, shedding light on its chemical constituents and their potential therapeutic effects. In vitro assays demonstrate the profound inhibitory effects of H. sanguineum extract on human fibroblast and breast cancer cells. Furthermore, we elucidate the underlying mechanisms of action, revealing its ability to induce apoptosis and cell cycle arrest in breast cancer cells. The cytotoxicity and apoptosis outcomes in breast cancer cells varied across different extracts, yet no adverse effects were observed on healthy cells at equivalent concentrations. Furthermore, all extracts initially promoted breast cancer cell proliferation, with the chloroform extract notably reducing cancer cell proliferation even at low concentrations. GC-MS analysis identifies the major chemical constituents of the extract, including flavonoids, terpenoids, and phenolic compounds, which likely contribute to its anticancer activity. Our findings highlight the potential of H. sanguineum extract as a natural agent for breast cancer treatment and the need for further exploration of its mechanisms and clinical applications.
Helichrysum genus, known as everlasting flowers or immortelles worlwide, is a member of the Asteraceae family represented by about 600 species over the world. Helichrysum plicatum DC., which is part of this genus, is an important figure in Turkey, Balkan Penunsula, and Iran because it is traditionally used for treatment of many diseases, especially diabetes, kidney stone, jaundice, and wounds. It is of great importance for Turkey due to hosting four subspecies of Helichrysum plicatum DC., which are Helichrysum plicatum DC. subsp. plicatum DC., Helichrysum plicatum DC. subsp. pseudoplicatum (Nab.) Davis & Kubicha, Helichrysum plicatum DC. subsp. polyphyllum (Lebed) Davis & Kubicha, and Helichrysum plicatum DC. subsp. isauricum Parolly. Flavonoids, pyrones, phloroglucinols, terpenoid, phtalides, and phenolic acids were detected in Helichrysum plicatum with a small number of studies. Likewise, monoterpenes, fatty acids, and sesquiterpenes were found in essential oil with limited number of phytochemical investigations. In recent year, the several pharmacological activities such as antioxidant, antimicrobial, antifungal, antiinflammaory, antidiabetic, antiurolithiatic, insecticidal, and anticancer activity have been confirmed by researchers. In this chapter, it is aimed that its traditional uses, phytochemistry and pharmacological properties of Helichrysum plicatum until date will be summarized and highlighted to explore the gaps and to contribute to future potential.Keywords
Helichrysum plicatum
AsteraceaeHelichrysum plicatum subsp. polyphyllumHelichrysum plicatum subsp. plicatumHelichrysum plicatum subsp. isauricumHelichrysum plicatum subsp. pseudoplicatumPharmacognosyPhytoconstituentsEthnopharmacology
Agastache spp. are used as ornamental plants for their pleasant aroma and the different colors of flowers. Nowadays, their edible flowers have become attractive for their nutraceutical properties. Post-harvest treatment appears as a crucial point to avoid impairment of the nutraceutical compounds and aroma, so different treatments were tested to analyze their effect on the bioactive metabolites and volatilome. Results indicated that freeze-drying was the best solution to prolong the shelf life of these flowers. The use of high temperatures (50, 60, 70 °C) led to altered the composition of antioxidant compounds (phenolic compounds, flavonoids, anthocyanins, carotenoids). Air-drying at 30 °C was a reasonable method, even though time consuming. Concerning the aroma profile, all samples were dominated by oxygenated monoterpene compounds. Pulegone was the main or one of the major constituents of all samples together with p-menthone. Gas Chromatography-Mass Spectrometry results showed a correlation between the temperature and the number of identified compounds. Both fresh and freeze-dried samples evidenced a lesser number (10 and 19, respectively); when the temperature raised, the number of identified constituents increased. Statistical analyses highlighted significant differences between almost all aromatic compounds, even if both Principal Component and Hierarchical Cluster analyses differed at 60 and 70 °C and from the other treatments.
Citrus fruits are cultivated and used as fresh fruits or for the production of juices Worldwide. These fruits are of great interest as raw materials for pharmaceutical, cosmetics and food industries due to their aromatic properties. Residues of the production of citrus juice, such as peels, membranes and seeds, possess a large variety of phytochemicals. In this study, it was aimed to prepare whole fruit powders of kumquat, limequat (a bigeneric hybrid) and Mexican lime excluding the seeds. The volatile compound profiles were determined by microdistillation. The cultivated mature fruits were harvested from Adana, Turkey in December 2017. Heat-treated or fresh mature fruits were freeze-dried. Then, each sample was pulverized in a blender and passed through a 100 μ sieve to obtain powders. Each sample was subjected to microdistillations. The volatile compounds were analyzed using GC-FID and GC-MS, simultaneously. Limonene was found to be the major component in all the samples.
Chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Anthemis cretica subsp. anatolica was analyzed by gas chromatography (GC) and gas chromatography-mass spectroscopy (GC-MS) systems. The essential oil of A. cretica subsp. anatolica was characterized by the presence of a high percentage of oxygenated sesquiterpenes (57.9%). Twenty-seven compounds were identified representing 96.6 % of the essential oil of A. cretica subsp. anatolica. The main components of the oil were spathulenol (27.0%) and hexadecanoic acid (14.3%).
In this study, volatile components by using Solid Phase Micro Extraction (SPME) method of Gold Grass (Helichrysum arenarium) collected in two different regions in June-August 2019 were determined by Gas Chromatography-Mass
Spectroscopy (GC-MS). While A total of 21 volatile components were detected in the sample of Golden Grass around
Bitlis Nemrut Crater Lake, a total of 33 volatile components were detected in the sample of Golden Grass in the
mountainous areas of Şebinkarahisar District of Giresun. While in the Golden Grass around Bitlis Nemrut Crater Lake
α-cedrene (26.65%), α-pinene (14.97%), α-humulene (10.65%), aromadendrene (6.79%), α-curcumene (6.31%),
germacrene B (4.43%), α-cadinene (3.82%), eucalyptol (3.57%), α-duprezianene (3.57%) and limonene (2.69%) were detected as the volatile main component, in the Golden Grass sample in the mountainous areas of Şebinkarahisar District of Giresun α-pinene (47.63%), α-himachalene (17.01%), α-humulene (5.21%), δ-cadinene (4.98%), γ-cadinene
(4.01%) and eucalyptol (3.46%) were detected as the volatile main component.
Helichrysum plicatum species are used in Turkish folk medicine as lithagogue, diuretic, and nephritic. Research on the methanol (MeOH) extract of flowers of H. plicatum DC. subsp. plicatum resulted in the isolation of eight known compounds (1-8). The chemical structures of the compounds were determined as β-sitosterol (1), apigenin (2), nonacosanoic acid (3), astragalin (4), β-sitosterol-3-O-β-D-glucopyranoside (5), helichrysin A (6), helichrysin B (7), and isosalipurposide (8) by spectroscopic and chromatographic/spectrometric methods, including 1D and 2D nuclear magnetic resonance and liquid chromatography-tandem mass spectrometry. Nonacosanoic acid (3) was isolated for the first time from H. plicatum DC. subsp. plicatum. The MeOH extract and isolated compounds were evaluated for their in vitro human carbonic anhydrase I (hCAI) and II (hCAII), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glycosidase inhibitory activities. The IC50 values of H. plicatum DC. subsp. plicatum MeOH extract for hCAI, hCAII, AChE, BChE, and α-glycosidase were found to be 77.87, 52.90, 115.50, 117.46, and 81.53 mg/mL, respectively. The compounds showed IC50 values of 1.43-4.47, 1.40-4.32, 1.69-2.90, 1.09-3.89, and 1.61-3.80 μM against hCAI, hCAII, AChE, BChE, and α-glycosidase, respectively. In summary, H. plicatum DC. subsp. plicatum secondary metabolites demonstrated strong inhibitory effects especially against hCAI and hCAII, whereas the MeOH extract showed a weak inhibitory effect on all enzymes.
