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| Digestive tract of giant borer (Telchin licus licus) and the diversity of colonies in solid BHI medium used for the isolation of bacteria. (A) The digestive tract being removed with the aid of forceps; (B) (B1) Bacteria obtained from the central region of the internodes, (B2) apoplast fluid, (B3) sugarcane roots, and (B4) digestive tract of the giant borer.
Source publication
Bacteria of the genus Bacillus can colonize endophytically and benefit several crops including the control of some pest orders. In view of the benefits provided by these microorganisms and in order to find out an efficient biotechnological control for the giant borer, our interest in studying the microorganisms in symbiosis with sugarcane and the g...
Citations
... The endophytic alkaloid metabolites and neurotoxins may lead to disordered behavior, hindered growth and development, and even death of insects (Song et al., 2020;Tooker and Giron, 2020;Grabka et al., 2022). For example, Bacillus in sugarcane can induce the abnormal development of giant borers, thus reducing diseases and insect pests (Rocha et al., 2021). ...
Endophytes is a kind of microorganism resource with great potential medicinal value. The interactions between endophytes and host not only promote the growth and development of each other but also drive the biosynthesis of many new medicinal active substances. In this review, we summarized recent reports related to the interactions between endophytes and hosts, mainly regarding the research progress of endophytes affecting the growth and development of host plants, physiological stress and the synthesis of new compounds. Then, we also discussed the positive effects of multiomics analysis on the interactions between endophytes and their hosts, as well as the application and development prospects of metabolites synthesized by symbiotic interactions. This review may provide a reference for the further development and utilization of endophytes and the study of their interactions with their hosts.
... Some sugarcane genotypes seem to differ in pest incidence, but truly resistant varieties have not been identified (Dinardo-Miranda 2008). Bacterial sub-species of the Bacillus thuringiensis Berliner (Bacillales: Bacillaceae) group have been isolated from the digestive tract of T. licus, suggesting the potential for using pathogenic strains of these bacteria against the pest (Rocha et al. 2021), although this approach has not yet been adequately explored. Application of the entomopathogen Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Hypocreales: Cordycipitaceae) via sub-surface drip irrigation systems has proven relatively effective in controlling T. licus in trials in Brazil (Krontal 2014), but such irrigation systems are rarely used in Colombia. ...
The giant sugarcane borer, Telchin licus (Drury) (Lepidoptera: Castniidae), also known as the banana stem borer, is an economically important pest of sugarcane in eastern Colombia. The presence of larval and pupal stages within stalks makes biological control of this pest difficult, and growers often resort to insecticide applications. Hilling up the soil around the base of plants, or soil mounding, is a cultural practice sometimes used to improve rooting in sugarcane. Because mature larvae cut holes in stalks close to ground level through which they can emerge as an adult, we hypothesized that soil mounding would impede adult emergence, and thus contribute to population reduction. Two experiments were conducted in fields with significant infestations of this pest in Puerto Lpez, Meta, Colombia, during the seasons of adult emergence in this region (AprMay and OctNov). Manual mounding of soil to a height of 20 cm was tested in the first trial, and mechanized mounding of soil in the second, which also compared 2 mounding heights (10 and 20 cm). In both cases, 2 m row transects of plants were caged to collect emergent adults. Adult emergence was reduced up to 65% in all mounding treatments, whether manual or mechanical, and regardless of mounding height, demonstrating that this cultural practice could be a useful tactic for inclusion in an integrated management program for this pest. However, as with any other cultural practice in pest management, region-wide implementation likely would be required to impact local population densities, and efficacy will depend further on low levels of moth immigration from alternative host plants.
Plant pests represent an increasing threat to the global food security. Although pesticides are conventionally controlled, their considerable damages to human and environment in addition to selecting resistant pathogens cannot be ignored. Consequently, alternative pest management techniques are more required for a sustainable farming and optimal functioning of the ecosystem. Biological control against plant pests based on the antagonistic ecological interactions between microbes and herbivorous insects has gaining more attention in last decades. Interactions between insects and microorganisms are modulated by the plant as a link between the above- and belowground biota. Plant-associated microbiota includes entomopathogenic microorganisms extensively used in pests’ management. Moreover, plant-associated microbes may enhance plant growth and defense system against herbivorous insects. Direct and indirect roles of plant-associated microorganisms in host-protection from pests’ attacks are well known. Molecular insights into plant-mediated interactions between microbes and insects, contributing to the enhancement of plant health and defense systems against insect aggression, spotted several research gaps related mainly to mechanisms underlying these interactions. Future prospects should mainly take the specificity of plant–microbe–insect interactions into consideration. This specificity may include genotypic and/or ecological aspects that should enhance the knowledge of the biocontrol mechanisms of insects by plant-associated microbes.
One of the urgent problems of plant protection from pests and diseases is the creation of environmentally safe biocontrol agents, the use of which would not be accompanied by an increase of the resistance of insect pests. Microorganisms have great potential in this regard. The most promising group are endophytes, which inhabit the internal tissues of plants and participate in formation of the phenotype of plant organisms. Bacteria of the genus Bacillus are of particular interest due to their wide distribution in the nature, the safety of many species for humans, and the relative ease with which biocontrol means based on Bacillus sp. could be obtained. The review considers the properties and activity of B. thuringiensis as follows: endophytic, insecticidal, antibiotic activity, production of growth regulators and mobilization of plant nutrients, resistance induction, as well as the possibility of constructing new strains using genetic engineering methods.
BACKGROUND
Bacillus thuringiensis (Bt) is a spore‐forming bacterium that produces insecticidal proteins and other virulence factors and is considered one of the most successful bioinsecticides available to control pests in agriculture. Currently, some Bt strains have been reported as endophyte or rhizospheric bacteria.
RESULTS
Little is known about the implications of plant‐Bt interaction in crop protection. Here, we review if Bt can establish as an endophyte/rhizobacterium and evaluate if Bt as an endophyte/rhizobacterium can simultaneously act against different phytopathogens (fungi, bacteria, insects and viruses) plus promote plant growth.
CONCLUSION
Although Bt produce an arsenal of proteins with toxic effects against insect, the current knowledge suggests that Bt can be considered as a promising new plant growth promotion bacterium (PGPB). The implications of the proposed review will broaden our understanding of Bt as a versatile entomopathogen that may be able to exhibit differential behavior depending on context. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Reduction of plant growth, yield and quality due to diverse environmental constrains along with climate change significantly limit the sustainable production of horticultural crops. In this review, we highlight the prospective impacts that are positive challenges for the application of beneficial microbial endophytes, nanomaterials (NMs), exogenous phytohormones strigolactones (SLs) and new breeding techniques (CRISPR), as well as controlled environment horticulture (CEH) using artificial light in sustainable production of horticultural crops. The benefits of such applications are often evaluated by measuring their impact on the metabolic, morphological and biochemical parameters of a variety of cultures, which typically results in higher yields with efficient use of resources when applied in greenhouse or field conditions. Endophytic microbes that promote plant growth play a key role in the adapting of plants to habitat, thereby improving their yield and prolonging their protection from biotic and abiotic stresses. Focusing on quality control, we considered the effects of the applications of microbial endophytes, a novel class of phytohormones SLs, as well as NMs and CEH using artificial light on horticultural commodities. In addition, the genomic editing of plants using CRISPR, including its role in modulating gene expression/transcription factors in improving crop production and tolerance, was also reviewed.
The use of endophytic bacteria in agriculture provides an effective way of improving crop yield and significantly reducing chemical usage, such as fungicides. This research was conducted to explore endophytic bacteria with plant growth promotion (PGP) and antifungal activities against Fusarium moniliforme AIT01. In this study, we obtained 52 isolates of endophytic bacteria associated with the roots and stems of sugarcane from Nakhon Ratchasima province, Thailand. In vitro antagonistic activity test showed that 14 out of 52 isolates had antagonistic activity against the fungal pathogen F. moniliforme AIT01. These antagonistic endophytic bacteria were identified as belonging to six different species as follows: Nguyenibacter vanlangensis, Acidomonas methanolica, Asaia bogorensis, Tanticharoenia aidae, Burkholderia gladioli and Bacillus altitudinis based on phenotypic characteristics, along with phylogenetic analysis of their 16S rRNA gene sequences. Seven isolates effectively inhibited F. moniliforme AIT01 mycelial growth by up to 40%. The volatile compounds of six isolates reduced the growth of F. moniliforme AIT01 by over 23%. Moreover, riceberry rice seedlings previously treated with B. gladioli CP28 were found to strongly reduce infection with phytopathogen by 80% in comparison to the non-treated control. Furthermore, the isolates also showed relevant PGP features, including ammonia production, zinc and phosphate solubilisation, auxin and siderophore biosynthesis. These results demonstrated that the tested endophytic bacteria could be successfully utilised as a source of PGP and biocontrol agent to manage diseases caused by F. moniliforme.
