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Differentiation of HUVECs using synthetic glycosaminoglycans A Schematic representation of the mixture of the synthetic glycosaminoglycans and growth factors (FGF2) with an elastin-like recombinamer (ELR) to give functionalized hydrogels. Synthetic glycosaminoglycans were conjugated to the ELR through ’click chemistry’. HUVEC cells were added on top and allowed to differentiate for 24 h. B Undifferentiated HUVECs when cultured in medium without supplements, characterized by cells clumping together and dying. C Differentiated HUVECs when stimulated with complete medium, characterized by the formation of tube-like structures (highlighted with white arrows). D, E Undifferentiated cells when stimulated with only FGF2 or only synthetic glycosaminoglycans. F Differentiated cells when stimulated with the combination of synthetic glycosaminoglycans and FGF2, (tube-like structures highlighted with white arrows).
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Healthy regeneration of tissue relies on a well-orchestrated release of growth factors. Herein, we show the use of synthetic glycosaminoglycans for controlled binding and release of growth factors to induce a desired cellular response. First, we screened glycosaminoglycans with growth factors of interest to determine kon (association rate constant)...
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