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Different growth stages of the leaf bud cuttings The maximum root length (8.89 cm) was recorded when the leaf cuttings were treated with IBA 100ppm + BA 0.0ppm. The minimum root length (6.74cm) was recorded in the treatment IBA 50 ppm + BA 50ppm, followed by (6.96cm) in the treatment IBA 25ppm + BA 50ppm. Significant variations were also recorded among the treatments for days to shoot initiation. Cuttings treated with IBA 100ppm + BA 0.0ppm took the minimum days (20.11) for shoot initiation whereas the maximum days (22.45) was taken by cuttings treated with IBA 0.0ppm + BA-50ppm. The maximum shoot length (4.37cm) was obtained in the treatment IBA 100 ppm + BA 0.0ppm and the minimum shoot length (2.70cm) in the treatment of IBA 25ppm + BA 10ppm.
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A new method of propagation of chrysanthemum through leaf cuttings complimentary to the conventional method of shoot tip propagation was developed under in vivo conditions. The study was conducted during 2008-2011 at the research farm of the Department of Floriculture and Landscaping, Punjab Agricultural University, Ludhiana, India. Two cultivars o...
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... this method, 10-15 new plants were produced from a single stem in contrast to the conventional method of propagation through terminal stem cuttings which yielded only one plant per stem. The rooted cuttings were transplanted into the field in the second week of June ( Fig. 1 and 2). ...
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Citations
... Therefore, this hybrid method is beneficial for plants that are difficult to root in the soil. Singh and Chettri (2013) highlighted that compared to the traditional method of multiplication by terminal stem cuttings, this innovative in vitro method of propagation by leaf cuttings boosted the rate of propagation of the plant by 10-15 times. Therefore, this method will help to overcome the minimal availability of herbal and medicinal plant samples through further improvements. ...
In natural medicinal system, the usage of herbs plays a vital role in curing various ailments. Baliospermum montanum L., a regionally vulnerable important herb needs conservation efforts. Studies have been conducted on the propagation through stems, seeds, and micropropagation. The availability of enough plants becomes a major criterion for raising medicinal plants on a large scale. This study aimed to investigate the role of different root-inducing substances and their rooting potential in single-leaf cuttings. In the first experiment, different aged leaves (old, mature, and young) were tried, and the results indicated that the mature leaves had a positive impact on all plant growth regulator (PGRs) cuttings, especially the auxins that were tested. No response was observed for other compounds such as honey, sugar, or undiluted tender coconut water (TCW). Leaf health scores were recorded based on changes in leaf color. The second experiment targeted varying levels of PGRs on the rooting parameters of the mature leaves. In terms of root numbers, IAA (500 ppm) produces 12.5 roots per leaf cutting followed by 11.1 in IAA (1,000 ppm) and 11.1 in IAA (200 ppm). It is superior to IBA and has a minimal response in the NAA. The root length was highest in IBA (1,000 ppm) immersed leaves at 13.6 cm followed by 12.4 cm and 9.8 cm in IAA 500 ppm and IAA 200 ppm respectively. Rooted leaves were planted in wet soil for further root and shoot growth. A reasonable rooting rate in IAA alone was observed after 7 weeks in the soil. So, we minimized the starting material through this leaf propagation procedure.
... Therefore, this hybrid method is beneficial for plants that are difficult to root in the soil. Singh and Chettri (2013) highlighted that compared to the traditional method of multiplication by terminal stem cuttings, this innovative in vitro method of propagation by leaf cuttings boosted the rate of propagation of the plant by 10-15 times. Therefore, this method will help to overcome the minimal availability of herbal and medicinal plant samples through further improvements. ...
In natural medicinal system, the usage of herbs plays a vital role in curing various ailments. Baliospermum montanum L., a regionally vulnerable important herb needs conservation efforts. Studies have been conducted on the propagation through stems, seeds, and micropropagation. The availability of enough plants becomes a major criterion for raising medicinal plants on a large scale. This study aimed to investigate the role of different root-inducing substances and their rooting potential in single-leaf cuttings. In the first experiment, different aged leaves (old, mature, and young) were tried, and the results indicated that the mature leaves had a positive impact on all plant growth regulator (PGRs) cuttings, especially the auxins that were tested. No response was observed for other compounds such as honey, sugar, or undiluted tender coconut water (TCW). Leaf health scores were recorded based on changes in leaf color. The second experiment targeted varying levels of PGRs on the rooting parameters of the mature leaves. In terms of root numbers, IAA (500 ppm) produces 12.5 roots per leaf cutting followed by 11.1 in IAA (1,000 ppm) and 11.1 in IAA (200 ppm). It is superior to IBA and has a minimal response in the NAA. The root length was highest in IBA (1,000 ppm) immersed leaves at 13.6 cm followed by 12.4 cm and 9.8 cm in IAA 500 ppm and IAA 200 ppm respectively. Rooted leaves were planted in wet soil for further root and shoot growth. A reasonable rooting rate in IAA alone was observed after 7 weeks in the soil. So, we minimized the starting material through this leaf propagation procedure.
... Due to the diversity of shapes and sizes of the inflorescence, the multitude of colors, its suitability for modern homes, make this flower species to be highly appreciated by the general public, and can be grown sustainably while simultaneously providing an economic benefit for growers. The dwarf and compact growing types (spray chrysanthemums) are cultivated as potted plants for decorating indoors and outdoors whereas the erect and tall growing types (standard chrysanthemums) are grown as cut flowers for bouquets and vase decoration (Singh and Chettri, 2013). Chrysanthemums are used either as cut flowers or grown in pots and the success in the cultivation of this plant is principally due to the great diversity of cultivars (Jamal Uddin et al., 2015). ...
Chrysanthemum is one of the top cultivated flowers, the undisputed queen of gardens in the autumn season. Due to its exceptional decorative qualities having a special range of shapes and colors, storage resistance, suitability in different
cultivation systems and the possibility of cultivation all over the year chrysanthemum is one of the most loved and appreciated flowers. It is one of the major crops
worldwide and it is cultivated as cut flowers and potted plants, depending on market demand. In Romania, nowadays, the cultivated areas with Chrysanthemum are
developing quickly through potted cultures which have gained a significant increase. Also, due to the progress made in the selection of chrysanthemums, many cultivars
have been introduced in the recent plant assortment. The present study was carried out to evaluate the influence of three rooting biostimulators on five potted chrysanthemums cultivars to determine the most efficient way to obtain a significant result for roots and aerial part’s growth and development. The greatest effects of rooting were obtained with Radistim 1 biostimulator. In which case was determined an increase of rooting percentage, number of roots, the height of plants, number of leaves and number of branches.
... maximum rooting (72.22%) and root length (6.42 cm). In chrysanthemum, the maximum root length (8.89 cm) was recorded when the leaf cuttings were treated with IBA 100ppm by dipping the basal portion of the cuttings for 15 minutes [35,36]. ...
Plant growth regulators consist of a large group of naturally occurring or synthetically produced organic chemicals and considered as a helping tool in the modern production system of ornamentals. Their exogenous application helps to improve the different economically important and market desirable characteristics of ornamental plants. The effects of PGRs in plants depend on various factors which play an important role to achieve expected results. These factors include the application method, time of application, concentration of PGRs, plant species and also the environmental conditions in which plants are grown. The intensity of applications is also considered an important factor affecting the efficacy of PGRs, as some plants respond well to a single application, but in most cases, multiple applications are beneficial to attain good results. This review briefly discusses the different areas in which PGRs are used in flower crops.
... Among the two planting materials studied, leaf bud cutting was found to be the best when compared to the single bud cutting. It is similar to the findings reported earlier that the propagation of chrysanthemum through leaf cuttings under in vivo conditions, increased the rate of regeneration of plants by 10-15 times as compared to the conventional method of propagation by terminal stem cuttings (Singh, and Chettri, 2013). Of the three growing media taken for study, enriched cocopeat followed by cocopeat + sand were identified as the best when compared to dam silt. ...
Proceedings of the 6th Agricultural Graduate Students Conference on “Multi-Dimensional Approaches in Transforming Agriculture” held at School of Post Graduate Studies, Coimbatore on May 28-29, 2020, TNAU Publications, Coimbatore – 641 003.
Chrysanthemum plant is considered one of the ornamental plants and cut flowers at the level of local and export production, recently. Alternaria leaf spot and blight disease are considered one of the most important diseases, which it has negatively effects on flower yield under greenhouse conditions with climate changes. This study confirmed the possibility of using plant extracts and bio-agents as a single component in an integrated control program to control Alternaria leaf spot and blight disease in chrysanthemum plants in Egypt. The disease was inventoried to the three regions of Heliopolis, (HelCa), in Darwa (MGQa) and Ezbat Al-Ahaly (MGQa) at El Qanater El Khaireya, which the symptoms were found in the form of brown spots and blight on the leaves and rotting of the flowers, also disease incidence (%) were 87.15, 61.44 and 38.72%, respectively. A total of 97 fungal isolates of Alternaria spp. were isolated. In vitro, the pathogenicity was tested by detached leaf inoculation using a single droplet technique over a period of 7-14 days, where the frequency of the high virulent isolates was (19.6%). The molecular identification was carried out using rDNA ITS for five highest pathogenic isolates, which the result was Alternaria alternata, where the process of cultural and morphological identification of MGQa-FYhd2(1-3) which the highest pathogenic isolate was completed. This isolate was tested with 10 different plant extracts that were extracted by methanolic extraction. Clude extract was the best result where EC 50 was 502.4 ppm and EC 90 was 281.6 ppm while the cinnamon extract expressed the EC 50 value of 1969.2 ppm and EC 90 was 10356.1 ppm. The bio-agents tesing showed the best treatments which inhibition of mycelial growth of the tested pathogen were Trichoderma harzianum (71.39%), and followed by Bacillus subtilis (69.41%).
Chrysanthemum plant is considered one of the ornamental plants and cut flowers at the level of local and export production, recently. Alternaria leaf spot and blight disease are considered one of the most important diseases, which it has negatively effects on flower yield under greenhouse conditions with climate changes. This study confirmed the possibility of using plant extracts and bio-agents as a single component in an integrated control program to control Alternaria leaf spot and blight disease in chrysanthemum plants in Egypt. The disease was inventoried to the three regions of Heliopolis, (HelCa), in Darwa (MGQa) and Ezbat Al-Ahaly (MGQa) at El Qanater El Khaireya, which the symptoms were found in the form of brown spots and blight on the leaves and rotting of the flowers, also disease incidence (%) were 87.15, 61.44 and 38.72%, respectively. A total of 97 fungal isolates of Alternaria spp. were isolated. In vitro, the pathogenicity was tested by detached leaf inoculation using a single droplet technique over a period of 7-14 days, where the frequency of the high virulent isolates was (19.6%). The molecular identification was carried out using rDNA ITS for five highest pathogenic isolates, which the result was Alternaria alternata, where the process of cultural and morphological identification of MGQa-FYhd2(1-3) which the highest pathogenic isolate was completed. This isolate was tested with 10 different plant extracts that were extracted by methanolic extraction. Clude extract was the best result where EC 50 was 502.4 ppm and EC 90 was 281.6 ppm while the cinnamon extract expressed the EC 50 value of 1969.2 ppm and EC 90 was 10356.1 ppm. The bio-agents tesing showed the best treatments which inhibition of mycelial growth of the tested pathogen were Trichoderma harzianum (71.39%), and followed by Bacillus subtilis (69.41%).
Single leaf-bud cutting has been attempted in an effort to produce multiple individuals; however, these cuttings produce individuals of irregular quality with different rooting abilities based on the cutting positions of the mother stem. We evaluated whether light and 1-naphthaleneacetic acid (NAA) treatment can improve root formation from relatively poor stem cuttings on single leaf-bud cuttings and which factor is more effective for rapid root formation. Blue (BL), red (RL), and fluorescent (FL) with/without NAA were independently applied to single leaf-bud cuttings. High-intensity BL together with NAA treatment efficiently induced adventitious root formation and improved root formation from poor stems. We also evaluated relative expression levels of seven kinds of rooting-related genes on cutting processes to define the critical light factor leading to rapid adventitious rooting. CmLBD1 was significantly upregulated after 5 day BL treatment and initiated rooting, whereas the expressions of other rooting-related genes were significantly increased by fluorescent light after 14 days cutting initiation.
Tanaman krisan (Chrysanthemum morifolium Ramat) merupakan tanaman perenial herbasius dari famili Asteraceae. Krisan memiliki nilai ekonomi yang cukup tinggi di Indonesia sebagai tanaman hias dalam bentuk bunga pot dan bunga potong. Permintaan pasar akan bunga krisan mengalami peningkatan setiap tahunnya.. Salah satu faktor yang mempengaruhi penurunan kualitas bunga potong krisan yakni etilen. Etilen merupakan hormon tanaman yang berperan pada proses pengguguran daun dan bunga, pemicu pembungaan, pembengkakan batang, pembentukan akar, memicu perkecambahan benih, pemasakan buah. Krisan termasuk kedalam jenis tanaman non-klimakterik, yakni tanaman yang kurang sensitif terhadap etilen. Namun pada beberapa penelitian menemukan beberapa varietas dari krisan sensitif terhadap paparan etilen eksogen. Salah satu cara untuk mengurangi efek etilen dengan aplikasi 1-Methylcyclopropene (1-MCP). Kerja 1-MCP pada tanaman yakni sebagai kompetitor etilen saat menempel pada reseptor. Aplikasi 1-MCP pada bunga potong krisan dapat memperpanjang umur dari bunga dengan mengurangi degradasi klorofil dan kehilangan berat segar bunga potong. Aplikasi 1-MCP pada setek krisan mampu mengurangi gejala kerusakan akibat etilen seperti penguningan daun, pengguguran daun, dan nekrosis. Setek yang diaplikasikan 1-MCP akan mengalami penghambatan dalam pembentukan akar karena terkait peran etilen terhadap inisiasi akar.
Chryzantema wielkokwiatowa zaliczana jest do najbardziej popularnych roś-lin ozdobnych na rynku ogrodniczym. Światowa produkcja tego gatunku rośnie z każdym rokiem. Skomplikowana biologia chryzantemy (wysoki poziom ploidalności, heterozygo-tyczność, poligeniczna kontrola kwitnienia, samoniezgodność oraz ograniczona żywotność pyłku) ogranicza jednak skuteczność tradycyjnych metod hodowlanych (krzyżowanie i selekcję). Dzisiaj, aby zaspokoić rosnące wymogi rynku w hodowli i rozmnażaniu tego gatunku, stosuje się nowoczesne osiągnięcia biotechnologii. W celu pozyskiwania nowych odmian wykorzystuje się hodowlę mutacyjną. W jej efekcie często uzyskuje się jednak chimery. Organizmy takie wymagają opracowania szczególnych warunków rozmnażania i przechowywania. Ostatnie dekady przyniosły jednak znaczący postęp w zakresie wy-korzystania kultur tkankowych w reprodukcji chryzantemy. Celem niniejszej pracy jest przedstawienie wybranych zagadnień z zakresu biologii oraz nowoczesnych metod hodow-li i reprodukcji chryzantemy wielkokwiatowej.
