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Diagram of similarities of tested microorganisms with respect to ethanol plant extracts made by dendritic method.
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The scope of the experiments included analysis of the antimicrobial activity of ethanolic, methanolic and aqueous extracts against bacterial and fungal cultures and determination of the minimum inhibitory concentration of plant extracts tested microbial growth. Analysis of the antifungal and antibacterial activity was carried out by the disc diffus...
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Fruits and vegetables are considered a beneficial contribution to the human diet. Especially, berries contain a great deal of bioactive compounds, such as anthocyanins, organic acids, tannins, phenols, and antioxidants. Apart from organic substances, inorganic nutrients are also present in fruits. Some metals and metalloids are essential for humans...
Thermal treatment of proteinaceous foods generates heat‐induced Maillard reaction substances including toxic advanced glycation end products (AGEs) and heterocyclic amines (HAs). It is known that plant phenolic compounds may influence Maillard reaction. This study investigated the impact of lingonberry leaf extracts on the formation of Nε‐(carboxym...
Aim of the study: To describe the effect of stand edge after clearcut on the process of Scots pine (Pinus sylvestris L.) natural regeneration along the edge-to-interior gradient. The density, height, horizontal structure and quality of natural regeneration was evaluated.
Area of the study: Kokořínsko Protected Landscape Area, Northern Bohemia (Cze...
Lingonberries (LB) have been shown to have beneficial metabolic effects, which is associated with an altered gut microbiota. This study investigated whether the LB-induced improvements were associated with altered gut- and neuroinflammatory markers, as well as cognitive performance in ApoE−/− mice fed high-fat (HF) diets. Whole LB, as well as two s...
Various wild berry species endemic to Alaska and the circumpolar North that exhibit unique medicinal properties have long been appreciated by indigenous Arctic communities. Traditional use of Alaskan berry preparations in the treatment of skin wounds is recorded but has not been scientifically evaluated. Alaskan wild berries feature diverse phytoch...
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... In addition, many of these compounds are effective against different resistant bacterial strains [10]. Thus, efforts to discover new antimicrobial agents from different types of natural sources are being made and supported by the increasing number of plant substances that can prevent and treat diseases [11,12]. ...
New sources of antibacterial drugs have become urgent with increasing bacterial resistance. Medicinal plants are attractive sources for antimicrobial compounds with fewer side effects and cheaper obtention. Brazil contains six biomes, including Caatinga, a semi-arid tropical vegetation exclusively from Brazil that contains over thousand vascular plant species. This review presents the potential of using Caatinga plant products to treat multidrug-resistant bacteria. This review used the keywords of antimicrobial resistance, resistance profile, multidrug resistance, Caatinga biome, and pathogenic bacteria to search in Scientific Electronic Library Online, the U.S. National Library of Medicine, and Google Scholar. Plant species as Schinopsis brasiliensis Engl., Annona vepretorum Mart., Croton pulegioides Baill., Myracrondruon urundeuva Allemo, Cereus jamacaru DC., Opuntia ficus-indica L., Bauhinia forficata L., Eucalyptus globulus, Croton sonderianus Muell. Arg., Campomanesia pubescens, and Abarema
cochliacarpos showed bacteriostatic activity. Encholirium spectabile Mart., Hymenaea courbaril L., Neoglaziovia variegata Mez, Selaginella convoluta Spring, Encholirium spectabile Mart., Bromelia laciniosa Mart., Hymenaea martiana, Commiphora leptophloeos, and Mimosa tenuiflora presented bactericidal activity. Those extracts inhibited clinical-importance bacteria, such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii. Therefore, Caatinga biome plants are a valuable source of active biomolecules against pathogenic bacteria, and their therapeutic potential must be further explored.
... The MIC endpoints were observed visually when viable cells changed their color from purple to pink (Markantonatou et al. 2020). Amikacin, fluconazole, and amphotericin B (Sigma) were used as positive controls for antibacterial, anti-Candia, and antifungal activities, respectively (Cioch et al. 2017;Manandhar et al. 2019;Rodrigues et al. 2019). ...
Yen NTN, Chung DD, Hong NTK, Cham NP, Nhan VT, Linh DTL, Ngoc NLB, Thai NM, Nga ND, Anh NT. 2023. Isolation, phylogenetic analysis and bioprospection of myxobacteria from Vietnam. Biodiversitas 24: 5653-5663. Myxobacteria have been considered microbial factories for producing secondary metabolites that have a variety of potential biological actions for discovering and isolating new biological molecules. Myxobacteria were isolated from soil samples collected in some provinces/cities in Vietnam. The purified isolates were identified based on morphology, biochemical test and phylogenetic analysis inferred from 16S rRNA gene. High-throughput screening assays including 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DDPH) for antioxidant properties and microdilution for antimicrobial activity were performed with myxobacterial extracts. Compounds from potential strain were predicted using liquid chromatography coupled with mass spectrometry. Forty-three myxobacterial strains were isolated and classified into seven genera of Angiococcus, Archangium, Chondromyces, Corallococcus, Cystobacter, Melittangium, and Myxococcus. The extract from CT21 strain had the highest total antioxidant activity (IC50 = 52.34 ± 1.47 and 30.28 ± 0.74 ?g/mL for the DPPH and ABTS, respectively). It is worth noting that all strains isolated myxobacterial strains show inhibitory activity against at least one of the tested microorganisms. The most potent antimicrobial strain was Myxococcus stipitatus GL41, which inhibited all tested microorganisms, and the minimal inhibitory concentration (MIC) values were 1 ?g/mL against methicillin-resistant Staphylococcus aureus (MRSA), methicillin-sensitive Staphylococcus aureus (MSSA), Streptococcus faecalis, Candida albicans, and Aspergillus niger. Mass spectrometry analysis revealed the presence of althiomycin - the polyketide antibiotic from ethyl acetate fraction. In the present study, myxobacteria were isolated from soil sample collected from Vietnam, analyzed phylogenetically, and screened for biological activities.
... The studies conducted so far on the antibacterial activity of plant extracts indicate that they, and compounds isolated from them, can be successfully used to inactivate microorganisms. The antibacterial effect of the extracts may be related to the modification of bacterial surface structures, inhibition of the synthesis of nucleic acids, and enzymatic and structural proteins [21,22]. Lingonberry is a medicinal plant whose leaves and fruits are valuable pharmaceutical raw materials because of their phenolic constituents. ...
... These substances have lipophilic properties, based on which they cause damage to the cytoplasmic membrane and the cell wall of microorganisms. In addition, they inhibit the synthesis of nucleic acids and enzymatic and structural proteins [21]. It was confirmed that flavonoids such as kaempferol, naringenin, quercetin, and apigenin were associated with a decrease in biofilm synthesis by disrupting quorum sensing. ...
... The composition of the lingonberry leaf extract used in our research has been described in our previous article [21]. The extract compounds were identified based on accurate mass searching, fragmentation analysis (MS/MS), comparison of accurate mass, and matching of the MS/MS pattern with standards and with data published in the literature. ...
European Union (EU) countries strive to improve the quality and safety of food of animal origin. Food production depends on a good microbiological quality of fodder. However, feed can be a reservoir or vector of pathogenic microorganisms, including Salmonella or Escherichia coli bacteria. Salmonella spp. and E. coli are the two most important food-borne pathogens of public health concern. Contamination with these pathogens, mainly in the poultry sector, can lead to serious food-borne diseases. Both microorganisms can form biofilms on abiotic and biotic surfaces. The cells that form biofilms are less sensitive to disinfectants, which in turn makes it difficult to eliminate them from various surfaces. Because the usage of formaldehyde in animal feed is prohibited in European countries, the replacement of this antibacterial with natural plant products seems very promising. This study aimed to assess the inhibitory effectiveness of Vaccinium vitis-idaea extract against biofilm produced by model Salmonella enterica and E. coli strains. We found that formaldehyde could effectively kill both species of bacterial cells in biofilm, while the lingonberry extract showed some antibiofilm effect on S. enterica serovar Senftenberg. In conclusion, finding natural plant products that are effective against biofilms formed by Gram-negative bacteria is still challenging.
... It is a garden plant and is grown as an ornamental shrub or small tree (Torosyan, 1983;Harutyunyan & Harutyunyan, 1986;Vardanyan, 2005). From the review of the available relevant literature, it can be seen that in some countries there is quite a lot of research aimed at studying the properties of the juice obtained especially from the generative organs of S. nigra (Cioch et al., 2017), as well as at studying the herbaceous life form species (Sambucus ebulus L.) of S. nigra, antioxidant, antibacterial activity of the plant (Rodino et al., 2015;Yahudin & Karomatov, 2016;Goud & Prasad, 2020). Similar studies are also found in Armenia (Vardanyan & Hovhannisyan, 2017). ...
We carried out studies on water deficiency of Sambucus nigra L. (black elderberry) plant leaves extract and fruit juice, relative turgidity, sucrose content in fruits, chlorophyll content in the leaves, as well as gas exchange rates in the leaves, in particular, the changes in the concentrations of oxygen released during photosynthesis by leaves and carbon dioxide released during leaf respiration depending on habitat conditions (altitude of the habitat, position of slopes, average annual precipitation). Taking into account the difference in climatic conditions and the generality of some indicators, Vanadzor (1326–1600 m above sea level) and Stepanavan (1400–1830 m above sea level) regions of Lori Province, "Dilijan" National Park, Lake Parz, and the forest areas adjacent to the city of Dilijan (1240–1612 m above sea level) in Tavush Province in Armenia were chosen as the research sites. The test samples were taken from eight different test sites in Lori and Tavush provinces. Tavush Province is more humid than Lori Province. The highest rate of water deficiency in the leaves of the studied plants was recorded in S. nigra f. laciniata (L.) Zabel., and the lowest in S. nigra in Vanadzor forest. It can be assumed that the index of water deficiency in the leaves may depend on the position of the slopes of the test sites and the value of the filtration coefficient of the soil types. On the north-facing slope at the height of 1341 m above sea level and on the south-facing slope at the height of 1830 m above sea level, the values of water deficiency of the leaves are almost the same. Such data allows us to assume that the degree of water deficiency in the leaves is also affected by the filtration coefficient of habitat soil types. In two points that have north-facing positions, the water deficiency of leaves at the height of 1341 m above sea level is 2.14 times more than at the height of 1328 m above sea level. At 1328 m above sea level the filtration coefficient is small and the plant absorbs a lot of water. Based on the data we received we obtained rankings of plants according to the different criteria of the experimental plants: water deficiency in leaves tissues, relative turgidity, the sum of chlorophylls in the leaves, the sucrose content in the fruits, percentage of oxygen released as a result of photosynthesis, percentage of carbon dioxide released as a result of respiration. We found a certain dependence between the indicators of the water regime and the height above sea level of the researched habitats. We also found out that the water regime is influenced by certain microclimatic conditions such as the position of the slopes, whether they are north or south facing, as well as the average annual precipitation. Knowing the physiological processes taking place in the vegetative and generative organs of the S. nigra plant is important from the point of view of evaluating the intensity of growth and development of the generative organs. We consider that the results of the study will be applicable in the process of plant selection based on the indicators of the physiological processes taking place in the plant organism.
... Due to their medicinal properties, historically, they have been used in traditional medicine to treat fever and oral and urinary tract infections [3]. Lingonberry fruit extracts have also been proven to be highly effective antimicrobial agents [4,5]. Lingonberries represent one of the most important and high-valued groups of modern-day "superfoods". ...
In recent years, much attention has been devoted to Vaccinium L. berries because of their substantial potential to be adapted for the development of innovative food and pharmaceutical applications. The accumulation of plant secondary metabolites is extremely dependent on climate and other environmental conditions. In order to increase the reliability of the findings, this study was conducted with samples collected in four regions in Northern Europe (Norway, Finland, Latvia, and Lithuania) and analyzed in a single laboratory using a standardized methodology. The study aims to provide a comprehensive understanding of the nutritional (biologically active compounds (phenolic (477–775 mg/100 g fw), anthocyanins (20–57 mg/100 g fw), pro-anthocyanidins (condensed tannins (141–269 mg/100 g fw)) and antioxidant activity in different systems (ABTS•+, FRAP). Physicochemical properties (acidity, soluble solids, color) of wild Vaccinium vitis-idaea L. were also evaluated. The results may contribute to the development of functional foods and nutraceuticals with potential health benefits in the future. To the best of our knowledge, this is the first comprehensive report on the evaluation of the biologically active compounds of wild lingonberries from different Northern European countries based on one laboratory’s validated methods. The results indicated a geomorphological influence on the biochemical and physicochemical composition of wild Vaccinium vitis-idaea L. depending on their place of geographical origin.
... Since plants are able to produce bioactive compounds to defend against plant pathogens, these compounds have been proven beneficial in the fight against certain microbes in humans as well. As such, plants are a promising source of natural products which can inhibit the growth and virulence of microbes (Cioch et al. 2017). Thus, plants may serve as a reservoir of antibiotic adjuvant to combat the issues of resistance (Wright 2016). ...
Appraised for being one of the oldest staple nutritive foods mainly in the Arabian Peninsula, the date palm tree (Phoenix dactylifera L.), is a crop native to the subtropical and tropical regions of Southern Asia and Africa. Different parts of the date tree have been extensively studied for their nutritional and therapeutic properties. Despite an array of publications on the date tree, there has been no attempt to compile in a single study the traditional uses, nutritive value, phytochemical profile, the medicinal properties as well as the potential of the different plant parts as a functional food. Therefore, this review endeavors to systematically review the scientific literature to highlight the traditional uses of date fruit and parts around the world, the nutritional profile of several parts and the medicinal properties. A total of 215 studies was retrieved (traditional uses (n = 26), nutritional (n = 52), and medicinal (n = 84)). Scientific articles were further categorized as in vitro (n = 33), in vivo (n = 35), and clinical (n = 16) evidences. Date seeds were found to be effective against E. coli and Staphylococcus aureus. Aqueous date pollen was used to manage hormonal problems and boost fertility. Palm leaves showed anti-hyperglycemic effects via inhibition of α-amylase and α-glucosidase. Unlike previous studies, this study highlighted the functional roles of all the plant parts of the palm tree and provided insights into the various mechanism of action of their bioactive compounds. Although scientific shreds of evidence have been growing over the years, there is still a dearth of studies concerning the clinical validation of the date fruit and other plant parts to provide strong evidence on their medicinal uses. In conclusion, P. dactylifera can be regarded as a potent medicinal plant with prophylactic potential and should be further explored to alleviate the burden of both communicable and non-communicable diseases.
... Medicinal plants contain a number of valuable substances, which can aid the prevention and treatment of several diseases. Due to contrast in the chemical composition of the mixture, biologically active substances show the activity of a different type than acting separately, which is the result of cooperative of their different elements [4]. Antimicrobials of plant emergence have expansive therapeutic potential. ...
The current research work is designed to determined phytochemicals and anti-microbial activities from selected seven plants based chemical agents namely Plantago major, Lilium polyphyllum, Rumex dentatus, Salvia moorcroftiana, Sophora mollis, Marrubium vulgare, Olea ferruginea. Different four chemicals (n-hexane, ethyl acetate, methanol, extract and distilled water) are used for extraction. The different extracts like n-Hexane, ethyl acetate, methanol, and distal water percentage extractive values of the seven selected medicinal plant were studied. The highest n-Hexane extractive values was recorded for Olea ferruginea 151% the next one was salvia moorcroftiana (Stem, flower) 128% then followed by Lilium polyphyllum and least for Rumex dentatus, Plantago major and salvia moorcroftiana (roots). The presence of alkaloids was recorded for Lilium polyphyllum, Rumex dentatus, Salvia moorcroftiana, Sophora mollis, Olea ferruginea While glycosides were present in Salvia moorcroftiana and Sophora mollis. Similarly Steroids and triterpenoid were present in Lilium polyphyllum, Salvia moorcroftiana (in stem, root) and Rumex dentatus. The tannins were absent in Olea ferruginea and Salvia moorcroftiana root while flavonoids were absent in Rumex dentatus, Plantago major and Olea ferruginea. Plantago major, Rumex dentatus, Sophora mollis, and Marrubium vulgare showed positive results for Phenols and Plantago major, Rumex dentatus, Salvia moorcroftiana showed the presence of protein. The plants extract are also tested for their antimicrobial activities against tested microbes, through well diffusion method. The results showed that three therapeutic plants (Rumex dentatus, Salvia moorcroftiana, Plantago major, Lilium polyphyllum (Distilled water extract) and Marrubium vulgare) demonstrated significant activities against tested microbes. While the remaining two plants (Olea ferruginea and Sophora mollis) no activities against tested microbes.
... However, they noted that the aqueous leaf extract had no activity against MRSA. In the same line, Cioch et al. [53] indicated that ethanolic, methanolic, and/or aqueous extracts of S. nigra flowers minimally inhibited the 11 species of microorganisms (eight bacteria and three fungi) studied. ...
Despite extensive research on the chemical composition of elderberries and their numerous uses in pharmaceutical, beverage, and food production, there is still a lack of knowledge about Sambucus nigra leaves and flowers’ antimicrobial activity against plant pathogens. In this study, the phytoconstituents of their aqueous ammonia extracts were first characterized by infrared spectroscopy and gas chromatography–mass spectrometry. The major phytocompounds identified in the flower extract were octyl 2-methylpropanoate; 3,5-dihydroxy-6-methyl-2,3-dihydropyran-4-one; propyl malonic acid; adenine; and 1-methyl-2-piperidinemethanol. Concerning the leaf extract, 1,6-anhydro-β-D-glucopyranose; oleic acid; 2,1,3-benzothiadiazole; 2,3-dihydro-benzofuran; and 4-((1E)-3-hydroxy-1-propenyl)-2-methoxyphenol and other phenol derivatives were the main constituents. The potential of the extracts to act as bioprotectants was then investigated against three almond tree pathogens: Diaporthe amygdali, Phytophthora megasperma, and Verticillium dahliae. In vitro tests showed higher activity of the flower extract, with EC90 values in the 241–984 μg·mL−1 range (depending on the pathogen) vs. 354–1322 μg·mL−1 for the leaf extract. In addition, the flower extract led to full protection against P. megasperma at a dose of 1875 μg·mL−1 in ex situ tests on artificially-infected excised almond stems. These inhibitory concentrations were lower than those of commercial fungicides. These findings suggest that S. nigra aerial organs may be susceptible to valorization as an alternative to synthetic fungicides for the protection of this important crop.
... В медицине бруника и красника могут применяться при лечении множества заболеваний: атеросклероза, нейродегенеративных расстройств, авитаминоза, гипертонии, почечнокаменной болезни, ревматизма, бронхиальной астмы, простудных заболеваний, стоматита, кожных болезней, диабета, заболеваний желудка, печени, мочеполовой системы и др. Ягоды брусники и красники обладают уникальными вкусовыми свойствами и ценностью в пищевом отношении: употребляются в сыром виде и служат сырьем при изготовлении соков, сиропов, компотов, варенья, джемов и кондитерских изделий [4,[6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23]. ...
Modern cost-effective propagation methods yield a large amount of high-quality healthy planting material of economically valuable forest berry plants. However, ex vitro adaptation of Vaccinium species and in vitro cultivation of the Kamchatka bilberry remain understudied. The research objective was to study the effect of growth-regulating substances on the organogenesis and adaptation to non-sterile conditions of the lingonberry and the Kamchatka bilberry during clonal micropropagation.
The study featured regenerant lingonberries (Vaccinium vitis-idaea L.) of Koralle, Kostromichka, and Kostromskaya Rozovaya cultivars, as well as the Sakhalin and Kuril varieties of the Kamchatka bilberry (Vaccinium praestans Lamb.). A chemical analysis was performed to reveal the following dependencies: the effect of sterilizing agents and sterilization time on the viability of explants, the effect of the nutrient medium and the growth-regulating substances on microshoots and roots, and the effect of the substrate on the survival of plants in non-sterile conditions.
The highest survival rate of lingonberry (72%) and bilberry (96%) explants belonged to 0.2% of AgNO3 with 10 min of sterilization time. The maximal values of the total shoot length in vitro were observed at 0.2 mg/L of 2-iP: AN nutrient medium (7.2 cm) for lingonberries and WPM 1/2 nutrient medium (10.5 cm) for bilberries. The longest total root length in vitro for lingonberries was registered when using 2.0 mg/L (5.8 cm) of indoleacetic acid, while for bilberries it was 1.0 mg/L (1.9 cm) of indolylbutyric acid. The maximal survival rate ex vitro belonged to the high-moor peat substrate (89–92%) for lingonberries and a 1:1 mix of peat with sand (91–95%) for bilb erries.
Clonal micropropagation with growth regulators (2-iP, indolylbutyric and indoleacetic acids) and peat substrates proved expedient for in vitro cultivation and ex vitro adaptation of the lingonberry and the Kamchatka bilberry. This scheme delivered a large amount of high-quality planting material with high plant survival.
... Numerous studies have been found that the medicinal plants which are used in traditional medicinal practices were inhibit growth and virulence of various microbes against infections that is potential antibacterial activity (Ahmad et al., 1998;Ahmad and Beg, 2001;Kumar et al., 2006;Bibi et al., 2011;Cioch et al., 2017) and few natural products have been approved as new antibacterial drugs (Kameshwara Rao, 1998;Subramani and Goraya, 2003). However, due to the widespread use of antibiotics, an increase in the incidence of antibiotic-resistant bacteria may render current antimicrobial medicines ineffective in controlling some bacterial infections. ...
