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Determination of necrosis marker by fluorescence microscopy of SYTOX green following doxazosin and carvedilol treatment. Doxazosin shows dose-dependent changes in the number of necrotic cells for 24 h. Carvedilol exhibit a lower number of cells dead related to the positive control for necrosis (HepG2 cells exposed to 250 μM hydrogen peroxide for 15 min). Pretreatment with curcumin maintains the viability of the cells because the staining is impermeant for apoptotic and live cells. Staining taken at 200x of magnification.
Source publication
Doxazosin and carvedilol have been evaluated as an alternative treatment against chronic liver lesions and for their possible role during the regeneration of damage caused by liver fibrosis in a hamster model. However, these drugs have been reported to induce morphological changes in hepatocytes, affecting the recovery of liver parenchyma. The effe...
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Context
Diabetic wounds (DW) are a complication of diabetes and slow wound healing is the main manifestation. Methylene blue (MB) has been shown to exhibit therapeutic effects on diabetes-related diseases.
Objective
To investigate the mechanisms of action of MB-nanoemulsion (NE) in the treatment of DW.
Materials and methods
The concentration of M...
Citations
... Curcumin has often been reported to exhibit a direct antioxidant capacity, which can relieve oxidative stress by scavenging ROS [32,33]. These could be attributed to the chemical structure of curcumin; a lipophilic polyphenol with a conjugated double bond serves as a powerful electron donor to inhibit the redox processes that produce ROS [34,35]. Thus, curcumin could improve the antioxidant capacity of broiler chickens infected with Coccidioides and maintain the equilibrium of the antioxidant enzyme system. ...
Simple Summary
Information about the effect of curcumin supplementation on the cecum of Eimeria tenella-infected broilers is scarce. This study examined the effects of curcumin on the growth performance, antioxidant system, intestinal integrity, and microbiota structure of broilers infected with E. tenella. Thus, this study provided a reference for using curcumin as a healthy feed additive.
Abstract
This study was conducted to investigate the effects of dietary curcumin supplementation on growth performance, anticoccidial index, antioxidant capacity, intestinal inflammation, and cecum microbiota in broilers infected with Eimeria tenella. A total of 234 one-day-old broilers were categorized into three treatments, with six replicates per treatment containing 13 broilers each. The three treatments included the control group, Eimeria tenella group, and Eimeria tenella + curcumin (200 mg/kg) group. The feeding trial lasted for 42 days, during which the broilers were orally administered with 0.9% saline or 5 × 10⁴ Eimeria tenella oocysts on day 14 of the study. On day 17 and day 21, one bird per replicate was selected for slaughtering. Results indicated an increased survival rate and anticoccidial index and improved productive performance in coccidia-infected broilers with curcumin supplementation. Furthermore, curcumin enhanced the serum antioxidant capacity in Eimeria tenella-infected broilers, evidenced by increased serum catalase activity (3d, 7d), as well as decreased malondialdehyde level (3d, 7d) and nitric oxide synthase activity (7d) (p < 0.05). Curcumin also improved intestinal inflammation and barrier function, evidenced by the downregulation of interleukin (IL)-1β (3d, 7d), TNF-alpha (TNF-α) (3d, 7d), and IL-2 (7d) and the up-regulated mRNA levels of claudin-1 (7d), zonula occludens (ZO-1; 3d, 7d), and occludin (3d, 7d) in the ceca of infected broilers (p < 0.05). Eimeria tenella infection significantly disrupted cecum microbial balance, but curcumin did not alleviate cecum microbial disorder in broilers infected with Eimeria tenella. Collectively, curcumin supplementation enhanced growth performance and anticoccidial index in Eimeria tenella-infected broilers via improving antioxidant ability and cecum inflammation without affecting cecum microbiota.
... For example, HepG2 cell viability remained about 80% after treatment with 15 μm doxazosin for 24 h, whereas about 60% of the HepG2 cells lost vitality after treatment with 25 μm doxazosin for 24 h. [34] Similarly, after treatment with 30 μm doxazosin for 24 h, about 78% of the PC-3 cells were viable. [17] In this study, we examined the pharmacotoxicity of doxazosin in HUVECs and HHSECs and found that the drug toxicity increased with increasing concentrations of doxazosin. ...
Liver fibrosis and cirrhosis, which are caused by chronic liver injury, represent common and intractable clinical challenges of global importance. However, effective therapeutics are lacking. Therefore, the study examines the effect of doxazosin on liver fibrosis. Carbon tetrachloride (CCl4) is injected into mice to establish a liver fibrosis model. Doxazosin (5 and 10 mg/kg) is administered daily by gavage. HE staining, Masson staining, Sirius Red staining, scanning electron microscopy, western blotting, real‐time PCR, and immunofluorescence analysis are performed to estimate liver fibrosis and sinusoidal capillarization in mice. Cell Counting Kit‐8 assays, western blotting, immunofluorescence analysis, tube formation, and transwell migration assays are performed on human umbilical vein endothelial cells (HUVECs) and human hepatic sinusoidal endothelial cells (HHSECs) to elucidate the potential mechanism of doxazosin. Doxazosin alleviates liver fibrosis and sinusoidal capillarization in CCl4‐induced mice. Angiogenesis is attenuated by doxazosin in HUVECs and HHSECs. This study demonstrates that doxazosin attenuated liver fibrosis by alleviating sinusoidal capillarization and liver angiogenesis.
... [7,9,17] These drugs are contraindicated in patients with liver failure because their chemical substances or derived metabolites can disrupt cellular homeostasis and exacerbate health problems due to their toxicity. [18,19] The role of the endoplasmic reticulum in the pathophysiology of various liver diseases, including hepatitis, fatty liver, and drug-induced hepatotoxicity, has recently gained recognition. It plays essential roles in the cell, including lipid biosynthesis, calcium storage, and the processes of protein synthesis, folding, assembly, trafficking, post-modification, and quality control. ...
... [32] Medina-Pizaño (2022) also reports that lower concentrations of carvedilol resulted in decreased cell viability, while higher concentrations even increased viability, suggesting a proliferative effect. [19] In our case, we observed a time-dependent negative effect on cell viability. This effect may be associated with the constitutive function of adrenergic receptors in hepatocytes, which play a role in modulating several functions such as carbohydrate, lipid, and amino acid metabolism. ...
The new anti-cancer and water-soluble curcumin derivatives have been synthesized solvent-freely by functionalizing the phenolic group of curcumin with various NH compounds to increase water solubility and cancerous properties. The biological applications of the new drugs have been investigated against the highly potent HepG2 carcinoma cell line. The studies showed that new curcumin derivatives could be used in low concentrations (µM/l<1) to avoid the anti-apoptotic properties against the cancerous cell. Also, the designed molecules with concentrations below 1 µM/l showed a good percentage of viability of 31.5, 45.3, and 66.7% against HepG2 cells. Furthermore, FTIR and 1H, and 13CNMR spectroscopy fully characterized the new curcumin derivatives.
Spinal cord injury (SCI) is an irreversible disease process with a high disability and mortality rate. After primary spinal cord injury, the secondary injury may occur in sequence, which is composed of ischemia and hypoxia, excitotoxicity, calcium overload, oxidative stress and inflammation, resulting in massive death of parenchymal cells in the injured area, followed by the formation of syringomyelia. Effectively curbing the process of secondary injury can promote nerve repair and improve functional prognosis. As the main active ingredient in turmeric, curcumin can play an important role in reducing inflammation and oxidation, protecting the neurons, and ultimately reducing spinal cord injury. This article reviews the effects of curcumin on the repair of nerve injury, with emphasis on the various mechanisms by which curcumin promotes the treatment of spinal cord injury.
