Fig 6- - available via license: Creative Commons Attribution 4.0 International
Content may be subject to copyright.
Detached endfeet, caused by loss of Myh10, impact cortical architecture
Source publication
Radial glial cells (RGCs) are essential for the generation and organization of neurons in the cerebral cortex. RGCs have an elongated bipolar morphology with basal and apical endfeet which reside in distinct niches. Yet, how this subcellular compartmentalization of RGCs controls cortical development is largely unknown. Here, we employ in vivo proxi...
Contexts in source publication
Context 1
... E16.5 Myh10 cKO brains almost all basal endfeet were detached from the BM and 400 absent from their niche in the marginal zone (MZ) [56]. We thus examined the general architecture of the MZ 401 which is populated by CR cells and interneurons (Fig 6A). First, we used DAPI staining to label all nuclei (Fig 402 6B). ...
Context 2
... we used DAPI staining to label all nuclei (Fig 402 6B). We discovered 33% more DAPI+ cells in the MZ of Myh10 cKO brains compared to Ctrl (Fig 6C). However, 403 the thickness of the MZ was unchanged indicating the influx of additional cells caused an overall higher cell 404 density (Fig 6D). ...
Context 3
... discovered 33% more DAPI+ cells in the MZ of Myh10 cKO brains compared to Ctrl (Fig 6C). However, 403 the thickness of the MZ was unchanged indicating the influx of additional cells caused an overall higher cell 404 density (Fig 6D). To assess the identity of these cells, we stained brains for calretinin, which labels both CR 405 cells and interneurons at this stage [57][58][59]. ...
Context 4
... assess the identity of these cells, we stained brains for calretinin, which labels both CR 405 cells and interneurons at this stage [57][58][59]. We quantified a 2.4 fold increase in the thickness of the calretinin 406 layer in the Myh10 cKO brains, suggesting that cells within this region may either be disorganized and/or 407 increased in number (Fig 6E,F). To determine if this defect preceded the endfoot phenotype, we assessed both 408 ...
Similar publications
The development of precise neural circuits in the brain requires spontaneous patterns of neural activity prior to functional maturation. In the rodent cerebral cortex patchwork and wave patterns of activity develop in somatosensory and visual regions, respectively, and are present at birth. However, whether such activity patterns occur in non-euthe...
