Figure 7 - uploaded by Ilona Judström-Kareinen
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Degranulated mast cells Light microscopy of a degranulated rat cardiac mast cells activated with compound 48/80 and stained with toluidine blue (0,1%), 40x magnification. Picture taken by Ilona Kareinen (30.06.2010)
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Atherosclerosis is an inflammatory disease characterized by the accumulation of cholesterol in the arterial intima and consequently the formation of atherosclerotic plaques. Formation of these plaques is initiated by the appearance of macrophage foam cell in the arterial intima. Foam cells are formed as excessive cholesterol accumulates in the cyto...
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... on their location, stage of maturation or species, mast cells express different types and levels of surface antigens and receptors, some of which are involved in activation and others in cell recognition ( Theoharides et al. 2012). Degranulation and the exocytosis of the intracellular granules are considered the hallmark of mast cell activation (Figure 7). The wide variety of surface receptors and their agonists is needed for an efficient and tissue-specific mast cell function. ...
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... ApoA-I proteolytic products were further characterized by blotting the proteins onto nitrocellulose membrane and detected by a polyclonal apoA-I antibody and the 16-4 mAb, which specifically reacts with truncated apoA-I at the carboxyl side of Phe 225 generated by human chymase. The human and rat chymase proteolytic products described above reacted with the polyclonal anti apoA-I antibody confirming them as apoA-I degradation products (IV, Figure 7). The 16-4mAB, however, reacted only with the apoA-I degradation product with apparent MW of 26kDa produced by human chymase (IV, Figure 7). ...
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... human and rat chymase proteolytic products described above reacted with the polyclonal anti apoA-I antibody confirming them as apoA-I degradation products (IV, Figure 7). The 16-4mAB, however, reacted only with the apoA-I degradation product with apparent MW of 26kDa produced by human chymase (IV, Figure 7). This suggested that the minor cleavage site Phe 225 was specific for human chymase. ...
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... further test this hypothesis, the effect of histamine on mRCT was evaluated in the atherosclerosis-prone LDLr-KO mice, having normal HDL levels but 10-fold higher levels of apoB-containing lipoproteins compared to the wild-type (III, Table 4). Histamine treatment in these hypercholesterolemic mice was unable to promote mRCT (III, Figure 7). Interestingly, the basal rate of mRCT appeared to be increased in LDLr-KO mice (0.45% of injected dose) (III, Figure 7C) compared to the wild-type mice (0.11%, in III; Figure 1C, and 0.10% in III; Figures 4B and 4C). ...
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... treatment in these hypercholesterolemic mice was unable to promote mRCT (III, Figure 7). Interestingly, the basal rate of mRCT appeared to be increased in LDLr-KO mice (0.45% of injected dose) (III, Figure 7C) compared to the wild-type mice (0.11%, in III; Figure 1C, and 0.10% in III; Figures 4B and 4C). It may well be, that histamine and as well as hypercholesterolemia induce vascular leakage ( Phinikaridou et al. 2013) and thus histamine effect becomes shunted in LDLr-KO mice. ...
