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![Decay of absorbance versus time by the cooxidation of the β-carotene/linoleic acid method. The values correspond to the mean ± SEM (n=3). BHT: 3,5-di-tert-butyl-4-hydroxy toluene; MC: methyl chavicol; MPMO: 2-[(4-methoxyphenyl)methyl]oxirane. The means differed from those of the negative control after analysis of variance followed by Tukey’s HSD (honest significant difference) test for ∗∗∗P<0.001.](publication/324465967/figure/fig3/AS:1086425630023698@1636035463812/Decay-of-absorbance-versus-time-by-the-cooxidation-of-the-b-carotene-linoleic-acid.jpg)
Decay of absorbance versus time by the cooxidation of the β-carotene/linoleic acid method. The values correspond to the mean ± SEM (n=3). BHT: 3,5-di-tert-butyl-4-hydroxy toluene; MC: methyl chavicol; MPMO: 2-[(4-methoxyphenyl)methyl]oxirane. The means differed from those of the negative control after analysis of variance followed by Tukey’s HSD (honest significant difference) test for ∗∗∗P<0.001.
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This study investigated the in vitro and in silico biological properties of the methyl chavicol (MC) and its analogue 2-[(4-methoxyphenyl)methyl]oxirane (MPMO), emphasizing the antioxidant and antilipase effects. MPMO was synthesized from MC that reacted with meta -chloroperbenzoic acid and, after separation and purification, was identified by ¹ H...
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... The difference between these data and ours could be explained by the lower content of the main component, the geographical and growth conditions described above, and the different analysis methods. Methyl chavicol is known to have pronounced antimicrobial, antioxidant, and biological activity [32][33][34]. It was found that, isolated from the essential oil of the species Agastache rugosa (Fisch. ...
Anise hyssop (Agastache foeniculum (Pursh) Kuntze) is a perennial plant belonging to the Lamiaceae family mainly used in folk medicine to treat various ailments. This study determined the chemical composition of the essential oil and evaluated its antimicrobial and antioxidant activity. The essential oil was obtained from plants cultivated in the experimental field at the Institute of Roses, Essential and Medical Plants, Kazanlak, Bulgaria. The plants were processed by steam distillation, with the essential oil yielding 0.37%, and its main components being methyl chavicol (82.03%) and limonene (9.90%). The most potent antimicrobial action was observed against the gram-positive bacteria Staphylococcus aureus (25.7 mm inhibition zone) and Bacillus cereus (12.3 mm), the yeast Saccharomyces cerevisiae (16.3 mm) and Candida albicans (16.5 mm). The remaining gram-positive bacteria (Staphylococcus epidermidis and Bacillus subtilis), gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, and Salmonella ebony), and fungi (Aspergillus brasiliensis and Fusarium moniliforme) were resistant to the action of the essential oil. The antioxidant activity of the essential oil was ABTS (32.36 µmol TE/mL), DPPH (21.61 µmol TE/mL), CUPRAC (19.94 µmol TE/mL), and FRAP (29.56 µmol TE/mL) in vitro. Overall, the results from this study revealed the biological potential of anise hyssop as a source in pharmaceutical, food, and cosmetic applications.
... The compound was methoxy derivatives of phenylpropane. Previous studies reported antioxidant, antilipase, insecticidal, anti-inflammatory, and antimicrobial activities as responsibility of MC [27]. In the present study, MC was found to possess an anti-streptococci activities against S. mutans and S. sobrinus, which are famous species of oral streptococci known to cause human caries. ...
Oral streptococci are the major group of bacteria in the oral cavity. Some of their species cause oral diseases that may lead to tooth loss and quality-of-life reduction, such as dental caries. One of prevention techniques to promote oral health is rinsing mouthwash after toothbrushing. This study aimed to determine the potential uses of local food, also remedy, plant in Thailand called Reaw-Horm or Etlingera pavieana for alternative herbal mouthwash. The essential oil from E. pavieana rhizome (Eo) is used for anti-streptococci including Streptococcus mutans and Streptococcus sobrinus and anti-biofilm activities. The main components of Eo are methyl chavicol (MC) and trans-anethole (TA). The disk diffusion method showed the inhibition zone of Eo in a dose-dependent manner. The minimum inhibitory concentration (MIC) of Eo and TA was >1.6 % v/v, and 0.4 % v/v of MC. Regarding anti-biofilm activities, MC showed nearly equal anti-biofilm formation of S. mutans and S. sobrinus, whereas Eo and TA acted toward S. sobrinus more than S. mutans biofilm. Sub-MIC killing effects on cells under biofilm were observed in Eo and MC. Therefore, MC was recommended as an active compound for anti-streptococci activities. Biocompatibility of Eo and MC were shown to be safe for epidermal cell lines. Herbal mouthwashes containing Eo were developed and had antioxidant and antimicrobial actions with established for 3 months. This study provides in vitro support on the use of herbal mouthwash with antioxidant and antimicrobial activities for dental caries prevention and well-being of individuals.
... Although impure samples can appear yellow, it is a colorless liquid, found in various trees and plants, including turpentine (pine oil), anise, fennel, bay, tarragon, and basil. Previous studies reported that ESG showed significant antioxidants [5], and antilipase [6], and blocks neuronal excitability via inhibiting the Na + channel [7]. It also showed an antiinflammatory effect via inhibiting leukocyte migration and by stimulating macrophage phagocytosis [8]. ...
... ESG is also known as methyl chavicol or p-allyl anisole, belonging to the class of phenylpropanoids found in essential oils of medicinal and food plants. It showed a wide range of pharmacological actions [5][6][7][8]. Given the outstanding pharmacological activity of ESG in earlier research, we have shown its pharmacological advantage and likely method of action against a rat model of arthritis caused by CFA in this investigation. ...
The present study was conducted to scrutinize the pharmacological effect of Estragole (ESG) against CFA-induced arthritis in rats. The rats underwent induction of arthritis using the administration of CFA and after that, the rats were randomly divided into five different groups, where three groups correspond to diverse dosages of ESG, and the other two were control and CFA-arthritic control. Results of the study suggested that ESG in a dose-dependent manner, improves body weight and arthritis score of rats as evidenced by reduction of hind-paw volume. ESG also improved the antioxidant status of rats by reducing MDA levels and enhancing the concentration of endogenous antioxidants SOD and GPx. The level of pro-inflammatory cytokines was also found to be reduced in the case of ESG treated group as compared to CFA-group. In a western blot analysis, ESH showed downregulation of p-JAK-2/STAT-3. The study provided concrete evidence for the protective effect of ESG against rheumatoid arthritis in rats.
... PL catalytic activity was measured at four different wavelengths: 400, [32,105,106] 405, [87,137,140] 410 [122] and 420 nm. [102] E. p-Nitrophenyl palmitate (pNPP) (12 studies) The pNPP used ranged between 0.12 [134] and 2.0 mM [113] . PL catalytic activity was measured at three different wavelengths: 400, [123] 405 [64,80,101,125,127,134,141] and 410 nm. ...
... PL catalytic activity was measured at three different wavelengths: 400, [123] 405 [64,80,101,125,127,134,141] and 410 nm. [94,111,113,115] F. 2,4-Dinitrophenyl butyrate (DNPB) (2 studies) ...
Obesity is a disease of epidemic proportions with a concerning increasing trend. Regarded as one of the main sources of energy, lipids can also represent a big part of an unnecessary intake of calories and be, therefore, directly related to the problem of obesity. Pancreatic lipase is an enzyme that is essential in the absorption and digestion of dietary fats and has been explored as an alternative for the reduction of fat absorption and consequent weigh loss.Literature describes a great variability of methodologies and experimental conditions used in research to evaluate the in vitro inhibitory activity of compounds against pancreatic lipase. However, in an attempt to choose the best approach, it is necessary to know all the reaction conditions and understand how these can affect the enzymatic assay.The objective of this review is to understand and summarize the methodologies and respective experimental conditions that are mainly used to evaluate pancreatic lipase catalytic activity.156 studies were included in this work and a detailed description of the most commonly used UV/Vis spectrophotometric and fluorimetric instrumental techniques are presented, including a discussion regarding the differences found in the parameters used in both techniques, namely enzyme, substrate, buffer solutions, kinetics conditions, temperature and pH.This works shows that both UV/Vis spectrophotometry and fluorimetry are useful instrumental techniques for the evaluation of pancreatic lipase catalytic activity, presenting several advantages and limitations, which make the choice of parameters and experimental conditions a crucial decision to obtain the most reliable results.
... The pancreatic lipase inhibition assay was performed according to Santos et al. [18] with some modifications. The porcine pancreatic lipase (10 g/L) was incubated in 50 mmol/L Tris-HCl buffer (pH 8.0) containing 10 mM CaCl 2 and 25 mM NaCl. ...
Over the last decades, the increased incidence of metabolic disorders, such as type two diabetes and obesity, has motivated researchers to investigate new enzyme inhibitors. In this study, the inhibitory effects of synthetic amino acid derivatives (PPC80, PPC82, PPC84, PPC89, and PPC101) on the activity of digestive enzymes were assessed using in vitro assays. The inhibitory effect was determined by the inhibition percentage and the 50% inhibitory concentration (IC50), and the mechanism of action was investigated using kinetic parameters and Lineweaver–Burk plots. PPC80, PPC82, and PPC84 inhibited pancreatic lipase (IC50 of 167–1023 µM) via competitive or mixed mechanisms. The activity of pancreatic α-amylase was suppressed by PPC80, PPC82, PPC84, PPC89, and PPC101 (IC50 of 162–519 µM), which acted as competitive or mixed inhibitors. Finally, PPC84, PPC89, and PPC101 also showed potent inhibitory effects on α-glucosidase (IC50 of 51–353 µM) as competitive inhibitors. The results suggest that these synthetic amino acid derivatives have inhibitory potential against digestive enzymes and may be used as therapeutic agents to control metabolic disorders.
... The chemical characterization of 38 basil genotypes resulted in seven groups: linalool (19-73%); linalool/eugenol (28-66% linalool and 5-29% eugenol); methyl chavicol (20-72% methyl chavicol); methyl chavicol /linalool (8-29% methyl chavicol and8-53%linalool);methyl eugenol/linalool(two accessionswith37%and91%methyleugenoland60% and 15% linalool); methyl cinnamate/linalool (9.7% methyl cinnamate and 31% linalool); and bergamotene (one accession with bergamotene as the major constituent) ( Among basil cultivars, seven forms were distinguished concerning leaf shape, size, and color, as well as plant height and habit (Wichtl, 2004). In turn, methyl chavicol is a phenylpropene substance (an isomer of anethole) with anti-lipase and anti-inflammatory e ects (Santos, 2018). Some toxic activity is related with this compound. ...
Sweet basil is cultivated for production of essential oils, dry leaves as a culinary herb, condiment/spice or as an ornamental plant. It is used as an ingredient in Ocimum basilicum L. commonly called as Sweet Basil belongs to family Lamiaceace is native plant of Indo-Malayan region. It is called the “king of herbs” which contains plenty of phytochemicals with significant nutritional as well as antioxidant capabilities and health benefits. Sweet basil is cultivated for production of essential oils, dry leaves as a culinary herb, condiment/spice or as an ornamental plant. It is used as an ingredient in various dishes and food preparations, especially in the Mediterranean cuisine. The unique array of active constituents called flavonoids found in basil provides protection at cellular level. Orientin and vicenin are two water-soluble flavonoids that have been of particular interest in basil. Essential oil of basil, obtained from its leaves, has demonstrated the ability to inhibit several species of pathogenic bacteria that have become resistant to commonly used antibiotic drugs. Due to its antimicrobial, insecticidal activity and very pleasant aroma, basil essential oil is widely used in the food, pharmaceutical, cosmetic, and aromatherapy industries. In addition, now-a-days public prefers natural food additives hence naturally derived antimicrobial agents from basil have become more important in antimicrobial packaging as they present a perceived lower risk to consumers. Aromatic leaves and flowering parts of O. basilicum are traditionally used as stimulant and tonic agents used in folk remedies to treat various ailments such as poor digestion, stomach-ache, feverish illnesses, nausea, abdominal cramps, gastro-enteritis, migraine, insomnia, depression, gonorrhoea, dysentery, and chronic diarrhoea exhaustion. Externally, they have been applied for the treatment of acne, loss of smell, insect stings, snake bites, and skin infections. Rosmarinic acid (RA) is the most biologically active compound present in Basil. The extract of the herb is used in preventing cardiovascular diseases through improved diet and several antioxidant compounds it contains display a high antioxidant power. The extracts have been shown to display important effects at the cellular level, including a platelet antiaggregant property and inhibitory activity against HIV to decrease plasma lipid content. Some of the medicinal properties that basil has are that it can be used to cure coughs, headaches, abdominal aches, and kidney diseases. Basil oils have been classified into four chemotypes according to their chemical composition and geographical source. The European type, cultivated in Europe, USA, and Africa, is characterized by linalool and methyl chavicol as the major oil constituents. The Reunion type, located in the Comoros and Seychelles Islands, Africa, and Reunion Island, is characterized by a high concentration of methyl chavicol. Tropical type originated from India, Pakistan, Guatemala, Haiti, and Africa is rich in methyl cinnamate. Another basil chemotype, with eugenol as the main component, is common in North Africa, Russia, Eastern Europe, and parts of Asia. In addition to these, other basil oils have also been reported which contained various quantities of linalool, camphor, methyl chavicol, methyl cinnamate, and eugenol.
... The pancreatic lipase inhibition assay was performed according to Santos et al. [18] with some modifications. The porcine pancreatic lipase (10 g/L) was incubated in 50 mmol/L Tris-HCl buffer (pH 8.0) containing 10 mmol/L CaCl2 and 25 mmol/L NaCl. ...
Over the last decades, the increased incidence of metabolic disorders such as type 2 diabetes and obesity has motivated researchers to investigate new enzyme inhibitors. In this study, the inhibitory effects of synthetic amino acid derivatives (PPC80, PPC82, PPC84, PPC89, and PPC101) on the activity of digestive enzymes was assessed by in vitro assays. The inhibitory effect was determined by the inhibition percentage and the 50% inhibitory concentration (IC50), and the mechanism of action was investigated by Lineweaver–Burk plots. PPC80, PPC82, and PPC84 inhibited pancreatic lipase (IC50 of 1.67–10.23 x 10-1 mmol/L). The activity of pancreatic α-amylase was suppressed by PPC80, PPC82, PPC84, PPC89, and PPC101 (IC50 of 1.62–5.19 x 10-1 mmol/L). Finally, PPC84, PPC89, and PPC101 also showed a potent inhibitory effect on α-glucosidase (IC50 of 0.51–3.53 x 10-1 mmol/L). PPC80 and PPC82 followed a non-competitive inhibition mechanism against pancreatic lipase, while PPC84 acted through competitive inhibition. The inhibition of pancreatic α-amylase by the derivatives was non-competitive, as well as for PPC84, PPC89 and PPC101 against α-glucosidase. The results suggest that these synthetic amino acid derivatives have inhibitory potential against digestive enzymes and may be used as therapeutic agents to control metabolic disorders.
... Knowledge of the qualitative and quantitative composition, depending on the fertilization and cultivar, is a very important aspect to obtain essential oil rich in specific important components for different medical purposes. For example, methyl chavicol, which has antioxidant and anti-lipase activities [57], was best produced by the cultivar 'Aromat de Buzau' under microorganism fertilization; β-linalool, with antimicrobial (e.g., Candida albicans, Staphylococcus aureus and Escherichia coli), antioxidant, anti-inflammatory and anticancer activities [58], had the highest content in 'Cuisoare' under chemical fertilization; and neral was only synthesized by 'Macedon', with the most remarkable production under organic fertilization. ...
The prospect of replacing traditional chemical fertilization with organic and microorganism-based fertilization meets the current demand for more sustainable cropping systems and healthy food. In this respect, research was carried out to evaluate the effects of the factorial combination between four basil cultivars (‘Aromat de Buzau’, ‘Macedon’, ‘Cuisoare’ and ‘Serafim’) and three types of fertilization, namely chemical fertilization (with a solid chemical fertilizer), organic fertilization (with chicken manure formulate) and microorganisms’ fertilization (with microorganisms formulate), on basil yield, biochemical and physiological parameters and essential oil composition. The results showed that the biometric parameters (plant height, number of stems and leaves and leaf area) were significantly influenced by the cultivar; ‘Macedon’ obtained the highest values of plant height (64.7 cm) and number of stems (20.33) and leaves (618.3) and ‘Serafim’ the largest leaf area (4901.7 cm2 per plant), while the type of fertilization did not affect these parameters. Regarding the biomass, the influence of the cultivar was not significant on fresh biomass but was significant on dry biomass, with ‘Macedon’ showing the highest value (56.4 g·plant−1 dry biomass). The mentioned parameters were significantly influenced by the type of fertilization, with the highest values recorded with chemical fertilization. Both the cultivar and the fertilization type significantly influenced the physiological parameters (the total content of assimilatory pigments and photosynthesis). Five phenolic compounds were quantified from leaf extracts by HPLC-MS (caffeic acid, hyperoside, isoquercitrin, rutin and quercitrin). Hyperoside was identified only in ‘Macedon’, while the rest of the compounds were found in all the cultivars and varied depending on the cultivar and fertilization type. Regarding the composition of the essential oil, variation was found depending on the cultivar and fertilization type. In ‘Aromat de Buzau’, the main compounds were methyl chavicol and β-linalool; in ‘Macedon’, geranial and neral; and in ‘Cuisoare’ and ‘Serafim’, β-linalool. Moreover, the PCA showed that the ‘Serafim’ cultivar has exclusive properties compared to the other cultivars. Our results highlight that identifying the most effective interaction between genotype and fertilization type allows to optimize yield and quality targets for sweet basil.
... A 96-well microplate reader measured the solution's absorbance at 630 nm (Perkin Elmer, EnSpire) at t = 0 min and t = 120 min. In order to calculate the percentage of pancreatic lipase inhibition, we used the following equations [36]: ...
Artemisia campestris L. is commonly used in folk medicine due to its antioxidant, antidia-betic, nutritional, and culinary properties. Our study assessed the total phenolics contents, antioxi-dant, and pharmacological activities of various organic extracts prepared from the aerial parts of Artemisia campestris, and its mineral elements and chemical profile were analyzed. ICP-OES was used to analyze the mineral profile and the LC-MS/MS analysis was used to characterize the phyto-chemical profiling. A series of antioxidant tests were carried out using DPPH, ABTS, beta-carotene, GOR, RP, CUPRAC, and O-Phenanthroline assays. In vitro potent inhibitory actions of A. campestris extracts were investigated to evaluate their anti-cholinesterase, anti-lipase and anti-diabetic activities. The photoprotective effect of the plant was measured by the sun protection factor. The most powerful inhibitor of α-amylase was AcPEE (IC50 = 11.79 ± 0.14 μg/mL), which also showed a significant butyrylcholinesterase inhibitory effect (IC50 = 93.50 ± 1.60 μg/mL). At IC50 = 23.16 ± 0.19 μg/mL, AcEAE showed the most powerful inhibitory effects on acetylcholinesterase. A. campestris was found to have a strong photoprotective ability, absorbing UV radiations with SPF values ranging from 26.07 ± 0.22 to 40.76 ± 0.11. The results showed that A. campestris extract has strong antiox-idant activity in all the test samples except for the carotene bleaching assay. The LC/MS-MS results showed that AcDE, AcEAE, and AcBE identified 11 compounds belonging to Polyphenols Compounds. Our result also showed that A. campestris contains a high concentration of essential minerals , including macro-and micro-elements with their values close to the FAO's recommended concentration. A. campestris has the capacity to improve pharmaceutical formulations, health, and medical research, due to its compositions and potent biological properties.
... Methyl chavicol is reported for antimicrobial action in essential oils and against phytopathogenic agents such as Brenneria nigrifluens [28]. Studies in silico have indicated that this compound has antilipase biological action and may be a promising molecular target for the treatment of diseases related to oxidative damage [29]. ...
Aedes aegypti L. (Diptera: Culicidae) is an important transmitter of diseases in tropical countries and controlling the larvae of this mosquito helps to reduce cases of diseases such as dengue, zika and chikungunya. Thus, the present study aimed to evaluate the larvicidal potential of the essential oil (EO) of Ocimum basilicum var. minimum (L.) Alef. The EO was extracted by stem distillation and the chemical composition was characterized by gas chromatography coupled with mass spectrometry (GC/MS and GC-FID). The larvicidal activity of EO was evaluated against third instar Ae. aegypti following World Health Organization (WHO) standard protocol and the interaction of the major compounds with the acetylcholinesterase (AChE) was evaluated by molecular docking. The predominant class was oxygenated monoterpenes with a concentration of 81.69% and the major compounds were limonene (9.5%), 1,8-cineole (14.23%), linalool (24.51%) and methyl chavicol (37.41%). The O. basilicum var. minimum EO showed unprecedented activity against third instar Ae. aegypti larvae at a dose-dependent relationship with LC50 of 69.91 (µg/mL) and LC90 of 200.62 (µg/mL), and the major compounds were able to interact with AChE in the Molecular Docking assay, indicating an ecological alternative for mosquito larvae control.
