Fig 4 - available via license: CC BY-NC-ND
Content may be subject to copyright.
Daphnetin enhances the activation of Nrf2/keap1 pathway in HG-stimulated MCs. MCs were incubated with or without daphnetin (10 or 20 mM) under NG or HG condition for 24 h. (A) The levels of Nrf2 and keap1 were analyzed by western blot. (B) Levels of Nrf2 and keap1 were quantified. MCs transfected with si-Nrf2 were incubated with daphnetin under HG. si-Nrf2 reversed the effects of daphnetin on ROS (C) and MDA levels (D) and SOD activity (E) in HG-stimulated MCs. *p < 0.05 vs. control cells, # p < 0.05 vs. cells stimulated with HG, & p < 0.05 vs. cells stimulated with HG þ daphnetion.
Source publication
Diabetic nephropathy (DN) is one of the most common causes of end-stage renal disease (ESRD). Oxidative stress and inflammation have been documented to play important roles in the pathogenesis of DN. Daphnetin, a natural coumarin compound, possesses antioxidant and anti-inflammatory activities. However, the role of daphnetin in DN has not yet been...
Contexts in source publication
Context 1
... is well known that Nrf2/keap1 and Akt/NF-kB pathways are two important inflammatory related pathways that are implicated in DN. Therefore, we further analyzed whether the pathways were involved in the effect of daphnetin. Western blot indicated that the HG induced the expression of Nrf2, and daphnetin enhanced the induction effect of HG (Fig. 4A). On the contrary, the keap1 levels were reduced by HG stimulation, and the reduction was stronger in the cells pretreated with daphnetin, indicating that daphnetin activated the Nrf2/keap1 pathway. On the other hand, the Nrf2-siRNA reversed the effects of daphnetin on ROS and MDA levels and SOD activity in HG-stimulated MCs (Fig. ...
Context 2
... effect of HG (Fig. 4A). On the contrary, the keap1 levels were reduced by HG stimulation, and the reduction was stronger in the cells pretreated with daphnetin, indicating that daphnetin activated the Nrf2/keap1 pathway. On the other hand, the Nrf2-siRNA reversed the effects of daphnetin on ROS and MDA levels and SOD activity in HG-stimulated MCs (Fig. ...
Citations
... DN is the main cause for end-stage renal disease with some clinical features, such as proteinuria and extracellular matrix accumulation 2 . Inseparable factors of DN include high blood glucose-induced inflammatory response and oxidative stress 3 . In terms of mechanism, certain pathways, such as Notch signaling and Hedgehog signaling, are been found to play a role in the development of DN 4,5 . ...
Aims/Introduction
Previous studies have shown that circular ribonucleic acid mediates the occurrence of diabetic nephropathy. This study aimed to analyze the effects of circ_0068087 on high‐glucose (HG)‐induced human kidney 2 (HK2) cell dysfunction.
Materials and Methods
Circ_0068087, miR‐580‐3p, and progestin and adipoQ receptor 3 (PAQR3) expression were detected by quantitative reverse transcription polymerase chain reaction. Cell viability and proliferation were investigated by Cell Counting Kit‐8 and EdU assays, respectively. The cell apoptotic rate was assessed by flow cytometry. Inflammatory response was assessed by enzyme‐linked immunoassays. Oxidative stress was evaluated by a superoxide dismutase activity assay kit and lipid peroxidation malondialdehyde assay kit. Molecular interaction was identified by dual‐luciferase reporter assay.
Results
Circ_0068087 and PAQR3 expression were significantly upregulated in diabetic nephropathy patients. HG treatment inhibited HK2 cell proliferation, but induced cell apoptosis, inflammation, oxidative stress and epithelial–mesenchymal transition by regulating circ_0068087. Circ_0068087 acted as a microribonucleic acid‐580‐3p (miR‐580‐3p) sponge, and miR‐580‐3p targeted PAQR3. Furthermore, circ_0068087 depletion repressed PAQR3 expression through miR‐580‐3p. MiR‐580‐3p inhibitors or PAQR3 introduction attenuated circ_0068087 silencing mediated‐effects in HG‐treated HK2 cells.
Conclusion
Circ_0068087 promoted HG‐induced HK2 cell injuries by the regulation of the miR‐580‐3p/PAQR3 pathway.
... In Figure Previous studies showed that HG could lead to in ammation [13] .In addition, DKD is widely considered as a kind of in ammatory disease. ...
Background Diabetic kidney disease(DKD) is one of the microvascular complications of diabetes. The total flavones of Abelmoschus Manihot (TFA) has been widely used in China to reduce podocyte injury in DKD, however, not each flavone monomer can play the same effect. In a specific disease or pharmacological model, there may also be a group of chemical components with clear composition and content ratio that can play the same role as Abelmoschus Manihot extract.
Methods The composition of the seven flavone monomers was investigated by spectrum-effect relationship and component knock-out and knock-in methods. We used HPLC to establish the chemical fingerprints, and assayed the protective effect and anti-inflammatory activity of podocytes in vitro. The protein expression level of synaptopodin and nephrin were measured by immunofluorescence staining, and the release of inflammatory factors of TNF-α and IL-1β were determined by enzyme-linked immunosorbent assay (ELISA) or real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). Partial least squares method and multi-objective optimization were used to examine the spectrum-effect relationships. Then, we also determined the expression of TRPC6 in podocytes by RT-qPCR and western blot.
Results When the compatibility proportion of rutin, hyperoside, isoquercetin, hibifolin, myricetin, quercetin-3'-o-glucoside, and quercetin was 0, 1.2, 0.88, 0.88, 0.48, 2.08, 0.4, the protein expression of synaptopodin and nephrin may be equivalent to TFA. And when the compatibility proportionof 7 active components was 0, 2.08, 1.74, 1.50, 0.12, 0.54, 0.1, which decreased the mRNA expression of TNF-α. Further, we found that B (hibifolin/hyperoside), C (hyperoside/quercetin-3'-o-glucoside), and E (isoquercetin/quercetin-3'-o-glucoside) have significant effects on synaptopodin, nephrin, and TNF- α, IL-1β. Collectively, our data indicated that TFA could ameliorate cytoskeleton rearrangement and inflammatory injury in podocytes under hign glucose conditions, and the proportion of hyperoside, isoquercitrin, hibifolin and quercetin-3'-o-glucoside had the similar efficacy, they may be the main active components of TFA. What’s more, the best combination of hyperoside, isoquercetin, hibifolin and quercetin-3'-o-glucoside decreased the expression of TRPC6.
Conclusion The combination of hyperoside, isoquercetin, hibifolin, and quercetin-3'-o-glucoside in TFA was optimized by spectral efficiency relationship, and flavone monomer combination may play a protective role in podocyte cytoskeleton through TRPC6 pathway.
... control of allergic rhinitis induced by ovalbumin in C57BL/6 female mice after oral treatment with 5 mg/Kg alleviated nasal symptoms, inflammatory response, and oxidative stress, activating Nrf2/HO-1 and inactivating the NF-κB pathway through the reduction in Toll-like Receptor 4, NF-κB protein levels, and TNF-α and IL-5 production [89]. Modulation of Nrf2 associated with other signaling pathways has been reported as the molecular mechanism of protection against several diseases, including cardiac hypertrophy and fibrosis [90], diabetes, lipid metabolism, and insulin resistance [91,92], hepatotoxicity [93], inflammation [88], lung toxicity [94], and neuronal and renal damage [95][96][97]. Protective antioxidant properties of daphnetin upregulating the Nrf2/HO-1 pathway with simultaneous inhibition of transforming growth factor β1 (TGF-β1)/Smad2/3 signaling axis was demonstrated in rat cardiomyoblast H9c2 cells and transverse aortic constriction model in C57BL/6 mice, alleviating cardiac hypertrophy and fibrosis [90]. ...
... Several in vitro studies corroborated the effects of daphnetin activating the Nrf2 signaling pathway and its action on other mechanisms, including the stabilization of antiapoptotic factor B-cell lymphoma-2 with simultaneous activation of adenosine-5′-monophosphate (AMP)-activated protein kinase, JNK, and ERK phosphorylation in human lung epithelial cells [94]; downregulation of the sterol regulatory element-binding protein-1C, patatin-like phospholipase domain-containing protein, cytochrome p450 2E1, and cytochrome P450 4A; upregulation of phosphoinositide 3-kinase (PI3K) and AMP kinase phosphorylation and increase of Akt levels to control lipid metabolism, insulin resistance, and oxidative stress in an in vitro model of non-alcoholic fatty liver disease [92]. Moreover, daphnetin inhibited glucose-induced extracellular matrix components, oxidative stress, and inflammation in human glomerular cells through Nrf2 activation and simultaneous inhibition of Akt and p65 levels [91]. Daphnetin also modulated the Nrf2 signaling pathway activating JNK and ERK with a subsequent increment of Nrf2 nuclear translocation Modulation of Nrf2 associated with other signaling pathways has been reported as the molecular mechanism of protection against several diseases, including cardiac hypertrophy and fibrosis [90], diabetes, lipid metabolism, and insulin resistance [91,92], hepatotoxicity [93], inflammation [88], lung toxicity [94], and neuronal and renal damage [95][96][97]. ...
... Moreover, daphnetin inhibited glucose-induced extracellular matrix components, oxidative stress, and inflammation in human glomerular cells through Nrf2 activation and simultaneous inhibition of Akt and p65 levels [91]. Daphnetin also modulated the Nrf2 signaling pathway activating JNK and ERK with a subsequent increment of Nrf2 nuclear translocation Modulation of Nrf2 associated with other signaling pathways has been reported as the molecular mechanism of protection against several diseases, including cardiac hypertrophy and fibrosis [90], diabetes, lipid metabolism, and insulin resistance [91,92], hepatotoxicity [93], inflammation [88], lung toxicity [94], and neuronal and renal damage [95][96][97]. Protective antioxidant properties of daphnetin upregulating the Nrf2/HO-1 pathway with simultaneous inhibition of transforming growth factor β1 (TGF-β1)/Smad2/3 signaling axis was demonstrated in rat cardiomyoblast H9c2 cells and transverse aortic constriction model in C57BL/6 mice, alleviating cardiac hypertrophy and fibrosis [90]. ...
Nrf2 (nuclear factor erythroid 2-related factor 2) is a transcription factor related to stress response and cellular homeostasis that plays a key role in maintaining the redox system. The imbalance of the redox system is a triggering factor for the initiation and progression of non-communicable diseases (NCDs), including Inflammatory Bowel Disease (IBD). Nrf2 and its inhibitor Kelch-like ECH-associated protein 1 (Keap1) are the main regulators of oxidative stress and their activation has been recognized as a promising strategy for the treatment or prevention of several acute and chronic diseases. Moreover, activation of Nrf2/keap signaling pathway promotes inhibition of NF-κB, a transcriptional factor related to pro-inflammatory cytokines expression, synchronically promoting an anti-inflammatory response. Several natural coumarins have been reported as potent antioxidant and intestinal anti-inflammatory compounds, acting by different mechanisms, mainly as a modulator of Nrf2/keap signaling pathway. Based on in vivo and in vitro studies, this review focuses on the natural coumarins obtained from both plant products and fermentative processes of food plants by gut microbiota, which activate Nrf2/keap signaling pathway and produce intestinal anti-inflammatory activity. Although gut metabolites urolithin A and urolithin B as well as other plant-derived coumarins display intestinal anti-inflammatory activity modulating Nrf2 signaling pathway, in vitro and in vivo studies are necessary for better pharmacological characterization and evaluation of their potential as lead compounds. Esculetin, 4-methylesculetin, daphnetin, osthole, and imperatorin are the most promising coumarin derivatives as lead compounds for the design and synthesis of Nrf2 activators with intestinal anti-inflammatory activity. However, further structure–activity relationships studies with coumarin derivatives in experimental models of intestinal inflammation and subsequent clinical trials in health and disease volunteers are essential to determine the efficacy and safety in IBD patients.
... Vriese et al. (Vriese et al. 2001) demonstrated that the treatment with the antibody of VEGF could ameliorate hyperfiltration, albuminuria, and glomerular hypertrophy in HFD/STZ-induced DM rats, suggesting VEGF could be a target for therapeutic intervention in DN. Additionally, the Akt and ERK1/2 signaling pathways were proven to be associated with DN (Xu et al. 2019). It has been reported that suppressing Akt could repress HG-induced inflammation and ECM procedure in mesangial cells (Xu et al. 2019). ...
... Additionally, the Akt and ERK1/2 signaling pathways were proven to be associated with DN (Xu et al. 2019). It has been reported that suppressing Akt could repress HG-induced inflammation and ECM procedure in mesangial cells (Xu et al. 2019). Multiple studies observed increased p-ERK1/2mediated hypertrophy and extracellular matrix accumulation in the DM model in vivo and in vitro (Mage et al. 2002;Fujita et al. 2004). ...
Background
Glabridin (Glab) is a bioactive component of licorice that can ameliorate diabetes, but its role in diabetic nephropathy (DN) has seldom been reported. Herein, we explored the effect and underlying mechanism of Glab on DN.
Methods
The bioactive component-target network of licorice against DN was by a network pharmacology approach. The protective effect of Glab on the kidney was investigated by a high-fat diet with streptozotocin induced-diabetic rat model. High glucose-induced NRK-52E cells were used for in vitro studies. The effects of Glab on ferroptosis and VEGF/Akt/ERK pathways in DN were investigated in vivo and in vitro using qRT-PCR, WB, and IHC experiments.
Results
Bioinformatics analysis constructed a network comprising of 10 bioactive components of licorice and 40 targets for DN. 13 matching targets of Glab were mainly involved in the VEGF signaling pathway. Glab treatment ameliorated general states and reduced FBG, HOMA-β, and HOMA-insulin index of diabetic rats. The renal pathological changes and the impaired renal function (the increased levels of Scr, BUN, UREA, KIM-1, NGAL, and TIMP-1) were also improved by Glab. Moreover, Glab repressed ferroptosis by increasing SOD and GSH activity, and GPX4 , SLC7A11 , and SLC3A2 expression, and decreasing MDA and iron concentrations, and TFR1 expression, in vivo and in vitro. Mechanically, Glab significantly suppressed VEGF, p-AKT, p-ERK1/2 expression in both diabetic rats and HG-induced NRK-52E cells.
Conclusions
This study revealed protective effects of Glab on the kidney of diabetic rats, which might exert by suppressing ferroptosis and the VEGF/Akt/ERK pathway.
... The nephroprotective mechanism of DAP is shown in Figure 4. A study also reported the preventive potential of DAP in diabetic nephropathy in mesangial cells at 10-40 µM by preventing cell proliferation, protection against oxidative stress and inflammation by targeting Nrf2/keap1, and Akt/NF-kB inflammatory pathways (Shen et al., 2017;Xu et al., 2019). ...
Daphnetin (DAP), a coumarin derivative extracted from Daphne species, is biologically active phytochemical with copious bioactivities including anti-inflammatory, anti-oxidant, neuroprotective, analgesic, antipyretic, antimalarial, anti-bacterial, anti-arthritic, neuroprotective, hepatoprotective, nephroprotective and, anti-cancer activities. It is reported to interact with multiple cellular mediators and signaling pathways to provide protection against neurodegeneration and arthritis. This review focuses on sources, synthesis, structure activity relationship, and various bioactivities of DAP. Neuroprotective and anti-inflammatory action of DAP is additionally aided by its modulation of the JNK-MAPK, JAK-STAT, and TLR-4/NF-κB signaling pathways. Although, the part of its anti-arthritic effect is mediated through immunoregulation, antioxidant and anti-inflammatory actions via regulation of NF-κB, MAPK and MMP signaling pathways, the anticancer action of DAP is mediated due to inhibition of Akt/ NF-Kb, MAPK signaling pathways, and the activation of Keap1-Nrf2 pathway. It is devoid of any organ toxicity and mortality as well as mutagenicity, mucosal irritation and sensitization reactions. Based on a review of the literature, DAP has a promising pharmacological and safety profiles and can be employed as a pharmaceutical moiety to treat a variety of illnesses. The current review intends to provide an in-depth focus on pharmacological activity and phytoanalytical approaches of DAP.
... Dracaena marginata is a well-known plant having the various pharmacological activity (Shen et al., 2018;Yu et al., 2014). Various parts K E Y W O R D S antioxidant, daphnetin, gut microbiota, hyperoxaluria, inflammation of the plant such as flower, roots, leaves and stems exhibited the various pharmacological effects includes relieving blood stasis, suppressing inflammation, removing blood stagnation to relive pain and boosting the blood circulation (Singh et al., 2021;Wang et al., 2019;Xu et al., 2019). The chemical phytoconstituents of the Dracaena marginata are lignans, coumarins and bisflavones. ...
It is well proved that hyperoxaluria induces the renal injury and finally causes the end stage kidney disease. Daphnetin (coumarin derivative) already confirmed renal protective effect in renal model, but hyperoxaluria protective effect still unexplore. The objective of this research was to scrutinize the renal protective effect of daphnetin against ethylene glycol (GC)‐induced hyperoxaluria via altering the gut microbiota. GC (1% v/v) was used for the induction of hyperoxaluria in the rats and the rats were received the oral administration of daphnetin (5, 10 and 15 mg/kg). The body and renal weight were assessed. Urine, renal, inflammatory cytokines, antioxidant, inflammatory parameters, and gut microbiota were appraised. Daphnetin effectually improved the body weight and reduced the renal weight. Its also remarkably boosted the magnesium, calcium, citrate level and suppressed the level of uric acid and oxalate formation. Daphnetin significantly (p < .001) ameliorate the level of urinary kidney injury molecule 1 (KIM‐1), blood urea nitrogen (BUN), urea, serum creatinine (Scr), neutrophil gelatinase‐associated lipocalin (NGAL) and uric acid along with inflammatory cytokines and inflammatory mediators. Daphnetin considerably repressed the malonaldehyde (MDA) level, protein carbonyl and improved the level of glutathione reductase (GR), superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT). Daphnetin treatment considerably altered the microbial composition of different bacteria at phylum, genus and family level. Daphnetin significantly suppressed the Firmicutes relative abundance and boosted the Bacteroidetes relative abundance. Our result clearly indicated that daphnetin remarkably ameliorates the GC induced hyperoxaluria in rats via altering the oxidative stress, inflammatory reaction and gut microbiota.
Practical application
Nephrotoxicity is a serious health disease worldwide. We induce the renal toxicity in the experimental rats using the ethylene glycol and scrutinized the renal protective effect of daphnetin. Daphnetin considerably suppress the renal, urine parameters. For estimation the underlying mechanism, we estimated the gut microbiota in all group rats. Daphnetin remarkably altered the level of gut microbiota and suggesting the renal protective effect.
... A total of 10 μL CCK-8 (Beyotime Biotechnology, Shanghai, China) solution was added to each well to avoid light, and the absorbance at 450 nm was determined after incubation for 3 h. The cell viability was expressed as a percentage (Xu et al., 2019). ...
We aimed to explore the effects of the ⁶⁰Co-γ irradiated ginseng adventitious root (GAR) with different radiation doses on the hypoglycemic effects of its extract (GARSE) through in vivo and in vitro experiments. The total saponin of GARSE was increased by 4.50% after 5 kGy irradiation, and the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability was enhanced by 5.10%. At 50 μg/mL, GARSE irradiated by 5 kGy displayed superior protective effects on human glomerular mesangial cells (HMCs) with high glucose damage. After feeding type 1 diabetes mellitus (T1DM) mice with GARSE irradiated by 5 kGy at 500 mg/kg•BW for 4 weeks, the glucose values was decreased by 16.0% compared with the unirradiated. The Keap1/Nrf2/HO-1 pathway was activated and the oxidative stress was attenuated, which further alleviated T1DM.
... Oxidative stress and inflammation caused by elevated blood Page 2 of 14 Sun et al. Diabetology & Metabolic Syndrome (2021) 13:141 glucose are considered to be inseparable factors in the occurrence of DN [6]. Hence, it is crucial to explore the mechanism of MC injury under high glucose (HG) for understanding DN development. ...
Background
The involvement of circular RNAs (circRNAs) in diabetic nephropathy (DN) has been gradually identified. In this study, we aimed to explore the functions of circRNA F-box/WD repeat-containing protein 12 (circ-FBXW12) in DN development.
Methods
Reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay was performed for the levels of circ-FBXW12, FBXW12 mRNA, microRNA-31-5p (miR-31-5p) and Lin-28 homolog B (LIN28B) mRNA. RNase R assay was used to analyze the stability of circ-FBXW12. Cell Counting Kit-8 (CCK-8) assay, flow cytometry analysis and 5-ethynyl-2′- deoxyuridine (EdU) assay were employed to evaluate cell viability, cell cycle and proliferation, respectively. Enzyme linked immunosorbent assay (ELISA) was done to measure the concentrations of inflammatory cytokines. Western blot assay was conducted for protein levels. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were examined with commercial kits. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were performed to verify the relationships among circ-FBXW12, miR-31-5p and LIN28B.
Results
Circ-FBXW12 level was increased in DN patients’ serums and high glucose (HG)-induced human mesangial cells (HMCs). Circ-FBXW12 knockdown suppressed cell proliferation, arrested cell cycle, reduced extracellular matrix (ECM) production and oxidative stress in HG-induced HMCs. Circ-FBXW12 was identified as the sponge for miR-31-5p, which then directly targeted LIN28B. MiR-31-5p inhibition reversed circ-FBXW12 knockdown-mediated effects on cell proliferation, cell cycle process, ECM production and oxidative in HG-triggered HMCs. Moreover, miR-31-5p overexpression showed similar results with circ-FBXW12 knockdown in HG-stimulated HMC progression, while LIN28B elevation reversed the effects.
Conclusion
Circ-FBXW12 knockdown suppressed HG-induced HMC growth, inflammation, ECM accumulation and oxidative stress by regulating miR-31-5p/LIN28B axis.
... AKT can accelerate the transcription of NF-κB (Gilmore 2006). Mounting studies have demonstrated that the AKT/NF-κB pathway is involved in the inflammation, oxidative stress, and ECM accumulation in DN (Ji et al. 2016;Xu et al. 2019Xu et al. , 2020. Xu et al. revealed that daphnetin reduced ECM accumulation, oxidative stress, and inflammation in HG-stimulated human mesangial cells by regulating the AKT/NF-κB and Nrf2/keap1 pathways . ...
Diabetic nephropathy (DN) is the main cause of end-stage renal disease. Circular RNA hsa_circ_0004442 (circTLK1) accelerates the progression of renal cell carcinoma. However, the role of circTLK1 in DN pathogenesis is indistinct. The expression of circTLK1, microRNA-126-5p (miR-126-5p), and microRNA-204-5p (miR-204-5p) was tested by quantitative real-time polymerase chain reaction. The levels of interleukin-6 and interleukin-1β were measured by enzyme-linked immunosorbent assay. The levels of reactive oxygen species and malondialdehyde and the activity of superoxide dismutase were determined with corresponding kits. Several protein levels were evaluated with western blotting. The relationship between circTLK1 and miR-126-5p/miR-204-5p was verified by dual-luciferase reporter assay. CircTLK1 was highly expressed in DN patient’s serum and high-glucose (HG)-treated human mesangial cells. Functionally, circTLK1 inhibition reduced HG-induced inflammation, oxidative stress, and ECM accumulation in human mesangial cells. CircTLK1 was verified as a sponge for miR-126-5p and miR-204-5p, which were downregulated in DN patient’s serum and HG-treated human mesangial cells. Both miR-126-5p and miR-204-5p upregulation decreased inflammation, oxidative stress, and ECM accumulation in HG-treated human mesangial cells and circTLK1 silencing-mediated influence on HG-induced human mesangial cell injury was overturned by miR-126-5p or miR-204-5p inhibition. Moreover, circTLK1 knockdown blocked the AKT/NF-κB pathway by sponging miR-126-5p/miR-204-5p. CircTLK1 downregulation alleviated HG-induced inflammation, oxidative stress, and ECM accumulation through blocking the AKT/NF-κB pathway via sponging miR-126-5p/miR-204-5p, providing a new mechanism to comprehend the pathogenesis of DN.
... where x t is the state variable, y t is the observer, η t is the observed noise, and u t is the increment between adjacent state variables. In order to reduce the space complexity of the Kalman filter, the covariance matrix of u t takes a diagonal matrix, which means that the motion/density changes between pixels are irrelevant [9,10]. The image frame at time t can be expressed as ...
In recent years, with the continued development and development of science and technology and the increasing maturity of medical technology, reactive nodular fibrous pseudotumors remain difficult to diagnose. Therefore, this paper studies CT and MRI imaging properties based on hypothetical gastrointestinal and mesangial reactive erythema nodosum fibrous pseudotumors to improve the diagnosis and treatment of reactive nodular fibrous pseudotumors. In this study, 48 patients with gastrointestinal and intermediate-reactive erythema nodosum fibrous pseudotumors in the gastrointestinal tract and experimental group were screened and identified by surgical pathology and immunohistochemistry. CT or magnetic resonance imaging was used in all patients. An analysis is performed, and the diagnostic values are compared with the pathological results. Experiments have proved that the postoperative reexamination of reactive nodular fibrous pseudotumors found that single masses are common, tumor sizes are different, and most small nodules have relatively more uniform lesion density, and the density of the plain scan is close to the muscle density, and the delay can be seen after enhancement. When the mass is large, the density is uniform, and low-density foci of different degrees can be seen in some masses, and this part rarely shows enhancement. The imaging phenomenon and clinical features of reactive nodular fibrous pseudotumors have specific features that distinguish them from invasive tumors such as gastrointestinal stromal tumors and provide an important basis for accurate clinical diagnosis and treatment.
