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Cytotoxic effect of crude scorpion venom on CRC cell line. The cell viability after treatment for 24hrs on the HT-29 CRC cell line with Hottentotta Saulcyi scorpion venom (Black scorpion) and Androctonus Crassicauda scorpion venom (yellow scorpion), respectively.
Source publication
This study was carried out to assess the impact of nickel nanoparticles (NiNPs) as well as scorpion venom on colorectal cancer (CRC) cells in the presence and/or absence of 5-fluorouracil (5-FU), hydrogen sulfide (H2S), and nitric oxide (NO) donors and to determine alterations in endothelial NO synthase (eNOS) and cystathionine γ-lyase (CSE) enzyme...
Context in source publication
Context 1
... cytotoxic effect of crude black scorpion venom (Androctonus Crassicauda) and yellow scorpion venom (Hottentotta Saulcyi) was conducted in a dose-dependent manner using CRC cell line (HT-29). The indirect measure of cell viability was determined using MTT assay. Both scorpion venoms showed no inhibition of cell proliferation after 24hrs treatment (Fig. ...
Citations
... The involvement of mutations in the CTH gene has been proposed in several types of cancer [9,10], but their precise contribution to the formation of GBM is still not well understood. The study of the CTH gene in GBM patients identified a combination of silent mutations and mutations that result in changes to amino acids. ...
... OR294044 and OR339875). Analysis and interpretation of the Sanger sequencing data were performed using the Mutation Surveyor software package 5.1 (SoftGenetics, LLC) by comparing the sequencing results with reference genes in public databases, including ClinVar, dbSNP, gnomAD and COSMIC (30). ...
The aim of the present study was to determine the cytotoxic effect of BAY-876 and NaSH alone or in combination with sunitinib against the 786-O cell line (renal adenocarcinoma). The IC50 of sunitinib, BAY-876 and NaSH were estimated. Cells were cultured in a 96-well plate and then different concentration of each drug alone was exposed for different incubation time; afterwards, cell cytotoxicity was measured using Cell Counting Kit-8 kit. The IC50 for each drug was used in next experiment to determine the influence of drug combinations. Furthermore, to observe the effect of mutations of few driver genes in development of clear cell renal cell carcinoma (ccRCC), direct sanger sequencing was used to find single nucleotide polymorphisms in exon 1 and exon 13 of tumor suppressor gene Von Hippel Lindau (VHL) and kinase insert domain receptor (KDR) genes respectively in ccRCC formalin fixed paraffin embedded block samples. The results revealed that the IC50 for sunitinib (after 72 h), BAY-876 (after 96 h) and NaSH (after 48 h) was 5.26, 53.56 and 692 µM respectively. The cytotoxic effect of sunitinib and BAY-876, sunitinib and NaSH combinations after 24- and 48-h incubation respectively was significantly higher (P<0.05) compared with the control group as well as to sunitinib group alone. These results proved that each of BAY-876 and NaSH have anticancer effect; thus, they could be used in future for ccRCC treatment purpose. Furthermore, direct sequencing results demonstrated unrecorded mutations of VHL and KDR genes is 43.7 and 31.5% of cases respectively. These findings confirmed the leading role of VHL gene in development of ccRCC and the crucial role of KDR gene in angiogenesis and drug resistance.
... Antioxidants, which act like a broom against free radicals, are recognized as effective treatments for destroying free radicals and regenerating damaged cells. More importantly, laboratory evidence suggests that antioxidants can significantly reduce cancer risk [57,[142][143][144]. Aqueous leaf extracts of C. officinalis, BHT, and NiO NPs have IC 50 of 328, 204, and 192 mg/mL, respectively ( Figure 19). ...
... The MNPs have high potential for DPPH inhibition. The results of our investigation are consistent with many other studies examining the mutual effects of herbs and metal salts on anti-oxidant capacity against DPPH[57,[142][143][144]. In general, MNPs have outstanding antioxidant properties, with the antioxidant properties of NiO NPs being well documented. ...
The two most prominent areas of nanotech-nology and nanoscience are environmental remediation and biomedical applications, which has a strong need to develop cleaner and more eco-friendly methods for preparing functional nanomaterials. This involves incorporating non-toxic chemicals and reagents for converting metal cations into metal or metal oxide nanoparticles (NPs), using readily available plant reagents and microbes as redox mediators. The extracts of these plants and microbes contain varying amounts of phytochemicals and metabolites that act as redox mediators and capping agents to stabilize bio-synthesized NPs. Considering these natural reagents for forming metal/metal oxide NPs, the present work aims to provide a complete review of the green fabrication of nickel oxide (NiO) NPs using extracts from various plant and microbial sources. In addition, the performance of various biosynthesized NiO NPs and their potential uses in biological applications are discussed.
... Furthermore, Housein et al. evaluated the effects of different combinations of nickel nanoparticles (NiNPs) with 5-FU, H 2 S, and NO donors on CRC cells. They found that H 2 S promoted the anticancer efficiency of 5-FU in the presence of NiNPs, while NO had anti-apoptotic activity on CRC cell lines [214]. This provides a new idea for H 2 S donor therapy in combination with chemotherapy. ...
Hydrogen sulfide (H2S) is an important signaling molecule in colorectal cancer (CRC). It is produced in the colon by the catalytic synthesis of the colonocytes' enzymatic systems and the release of intestinal microbes, and is oxidatively metabolized in the colonocytes' mitochondria. Both endogenous H2S in colonic epithelial cells and exogenous H2S in intestinal lumen contribute to the onset and progression of CRC. The up-regulation of endogenous synthetases is thought to be the cause of the elevated H2S levels in CRC cells. Different diagnostic probes and combination therapies, as well as tumor treatment approaches through H2S modulation, have been developed in recent years and have become active area of investigation for the diagnosis and treatment of CRC. In this review, we focus on the specific mechanisms of H2S production and oxidative metabolism as well as the function of H2S in the occurrence, progression, diagnosis, and treatment of CRC. We also discuss the present challenges and provide insights into the future research of this burgeoning field.
... 9 For example, when the effect of gasotransmitters such as NO and H 2 S was studied on colon rectal cancer cells, the NO donors showed antiapoptotic effects, whereas the H 2 S donors exhibited a decrease in proliferation rate. 10 Although the gasotransmitters have proven to be of great therapeutic value, delivering the molecule directly to the target presents several difficulties. There are many small-molecule precursors of gasotransmitters which can release the gas under physiological conditions, but the drug donors are easily disintegrated in vivo before reaching the target sites, reducing the bioavailability of the gasotransmitters. ...
Gasotransmitters such as NO, H2S, and CO have emerged as key players in the regulation of various pathophysiological functions, prompting the development of gas therapy for various pathogeneses. Deficient production of gasotransmitters has been linked to various diseases such as hypertension, endothelial dysfunction, myocardial infarction, ischemia, and impaired wound healing, as they are involved in the regulatory action of angiogenesis. A better understanding of the regulatory mechanisms has given new hope to address the vascular impairment caused by the breakthroughs in gasotransmitters as therapeutics. However, the unstable nature and poor target specificity of gas donors limit the full efficacy of drugs. In this regard, biomaterials that possess excellent biocompatibility and porosity are ideal drug carriers to deliver the gas transmitters in a tunable manner for therapeutic angiogenesis. This review article provides a comprehensive discussion of biomaterial-based gasotransmitter delivery approaches for therapeutic angiogenesis. The critical role of gasotransmitters in modulating angiogenesis during tissue repair as well as their challenges and future directions are demonstrated.
... Antioxidants acted against free radicals and were recognized as powerful therapies to destroy free radicals and regenerate damaged cells. More importantly, laboratory findings have shown that antioxidants can significantly prevent cancer [42]. ...
Over the past several years, the greener fabrication of metal oxide nanoparticles has attracted significant attention due to their simplicity, eco-friendliness, availability, and nontoxicity. This paper focused on the fabrication of nickel oxide nanoparticles (NiO-NPs) using the leaf extract of Ziziphus spina-christi L. and evaluating its potential biological activities. The characterization of synthesized NiO-NPs was confirmed using ultraviolet-visible spectroscopy, field emission-scanning electron microscope, energy-dispersive X-ray spectroscopy, and X-ray diffraction. Furthermore, protoscolicidal, antibacterial, and antioxidant activities and hemocompatibility of NiO-NPs were investigated. The findings revealed that the NiO-NPs were crystalline on nanoscale between 50-and 90-nm particle sizes. The NiO-NPs showed high scolicidal activity against Echino-coccus granulosus. The viability of the treated protoscoleces exponentially decreased with an increase in the concentration of NiO-NPs. The NiO-NPs exhibited effective antibacterial activity against Escherichia coli and Staphylococcus aureus. NiO-NPs also possess a H 2 O 2 scavenging activity in a dose-dependent manner. This study revealed that the Z. spina-christi L. leaf extract is an effective reducing and capping agent for the production of NiO-NPs; it showed critical biological properties. Moreover, NiO-NPs have a potent antioxidant activity and low toxicity on the erythrocytes and appear hemocompatible.
... Ni and Cu are known metal centres surrounded by macrocyclic ligands in enzymes such as urease, nitrite reductase, laccase, hydrogenase, etc. These enzymes participate in the metabolic pathways of NO in the human body [13][14][15][16]. As much as NO electrochemical sensors have been found to be applicable in the detection of NO in vivo and in vitro, the design, use, and limit of detection of the sensor materials remain a challenge [17][18][19]. ...
Nitric oxide (NO), the smallest signalling molecule known in the human body, keeps blood vessels dilated, controls blood pressure, and has numerous other health regulatory effects. The use of Schiff base complexes incorporated onto electrodes to make electrochemical sensors has been explored as an effective method for the determination and quantification of nitric oxide in aqueous solutions. Schiff base ligands were complexed with Cu and Ni metal centres using the microwave synthesis method to produce metal–ligand complexes with enhanced capabilites for NO detection. The electrical current generated at the anode is directly proportional to NO concentrations in the solution through its oxidation to HNO3. Various characterisation techniques were implemented to verify the integrity of each step of metal–ligand synthesis as well as the final product produced, using FT-IR, UV-VIS, and TGA. The as-synthesised Schiff base complexes were electrodeposited on screen-printed carbon electrodes (SPCE) and electrochemically evaluated in a 0.1 M PBS. Furthermore, metal complexes were screened for their in vitro activity towards NO detection in an aqueous solution (PBS). The results show that the investigated sensors (SPCE/Ni-BPND and SPCE/Cu-BPND) respond positively toward NO detection. It was, therefore, identified that the two sensors also do not differ significantly in terms of precision, sensitivity, and lowest detection limit. The sensor strategies demonstrate the NO limits of detection of 0.22 µM and 0.09 µM, and they also demonstrate sensitivity values of 16.3 µA/µM and 13.1 µA/µM for SPCE/Cu-BPND and SPCE/Ni-BPND sensors, respectively.
... In fact, the green synthesized metallic nanoparticles have achieved notable consideration in a variety of medicinal disciplines [9][10][11][12]. Some relevant conducted studies have shown that some nanoparticles (especially platinum, gold, and silver nanoparticles) have promising therapeutic properties, accounting for their potential use as excellent alternatives to physiochemically different metal-supported nanoparticles, antibacterial, and particularly anticancer drugs (such as cisplatin and azathioprine) [10][11][12][13][14][15][16][17]. ...
... Direct sequencing reveals new mutations, mainly of intronic variation in the endothelial NO synthase gene, which has not previously been described in the database. These findings indicate that H 2 S promotes the anticancer efficiency of 5-fluorouracil in the presence of nickel nanoparticles, while NO has antiapoptotic activity in colorectal cancer cell lines [16,17]. A study by Chen et al. revealed the anti-immortalized myelogenous leukaemia activities of metallic nanoparticles green-synthesized by the Tsoong herb against K562 cell line under in vitro conditions. ...
... Antioxidants act like a broom against free radicals and have been recognized as powerful remedies to destroy free radicals and regenerate the damaged cells. More importantly, laboratory evidence has shown that antioxidants can effectively prevent cancer [13][14][15][16]. The IC 50 of BHT and FeNPs were 91 and 132 µg/ml, respectively ( Figure 4; Table I). ...
Introduction
Recently, scientists have understood that metallic nanoparticles especially iron nanoparticles have excellent anticancer properties. A green, productive, and environmentally method was developed for the valuable study and the effective preparation of the biosynthesis of iron nanoparticles using aqueous extracts from the leaf of Coriandrum sativum as a result of reducing and stabilizing factor. The simplicity of the reaction, heterogeneous system, and easy work up are the benefits of the present method.
Material and methods
The as-prepared nanoparticles (FeNPs) was characterized using UV-Vis, SEM, and FT-IR. It has been shown that the iron nanoparticles have spherical shape and uniform size.
Results
The synthesized nanoparticles had very low cell viability and high anti-liver cancer activities dose-dependently against pleomorphic hepatocellular carcinoma (SNU-387), hepatic ductal carcinoma (LMH/2A), morris hepatoma (McA-RH7777), and novikoff hepatoma (N1-S1 Fudr) cell lines without any cytotoxicity on the normal cell line (HUVEC). The synthesized nanoparticles inhibited half of the DPPH molecules in the concentration of 132 µg/mL. Perhaps notable anti-liver cancer activities of the synthesized nanoparticles against common liver cancer cell lines are linked to their antioxidant activities.
Conclusions
Our results point out that the FeNPs from Coriandrum sativum extract are apposite stabilizing agents, which serve as an effective anticancer agent against liver cancer cell lines.
... The IC 50 values for GS FeONPs and CS FeONPs in radical scavenging activity is presented in Table 1. Generally metal oxide nanoparticles show remarkable radical scavenging activities by releasing oxygen (Al Jahdaly et al., 2021) which has been well reported (Housein et al., 2021). When NPs are synthesized using plant extracts rich in phenolic and flavonoid compounds, the antioxidant activity of such NPs are even more enhanced (Zhang et al., 2021). ...
Iron oxide nanoparticles synthesis is an expanding area of research due of their magnetic properties and possible applications in several novel technologies. FeONPs are indispensable in the biomedical field for diagnosis, treatments and drug delivery and in bioremediation applications. The synthesis route of nanoparticles is a major concern because biological methods are eco-friendly, and chemical methods are considered toxic. The objective of this study is to synthesize FeONPs by two different methods and to compare their properties and efficiency in applications. FeONPs were synthesized and characterized by microscopic and various spectroscopic techniques. The synthesized FeONPs were screened for their cytotoxic activity on PBMCs using MTT assay and found to exhibit good biocompatibility. Moreover, the GS FeONPs exhibited potential antibacterial activities and meanwhile showed less toxicity in brine shrimp lethality assay. Hence, these nanoparticles are biocompatible, environmentally safe and can be utilized in many medical applications.
... Direct sequencing reveals new mutations, mainly intronic variation in endothelial NO synthase gene that has not previously been described in the database. These findings indicate that H 2 S promotes the anticancer efficiency of 5-fluorouracil in the presence of nickel nanoparticles while NO has antiapoptotic activity in colorectal cancer cell lines [16]. ...
... Antioxidants act like a broom against free radicals and have been recognized as powerful remedies to destroy free radicals and regenerate the damaged cells. More importantly, laboratory evidence has shown that antioxidants can highly prevent cancer [13][14][15][16].The IC 50 of C. officinalis leaf aqueous extract, BHT, and NiONPs were 328, 204, and 192 µg/mL, respectively ( Figure 6; Table 2). ...
... Metallic nanoparticles also have excellent potential to inhibit DPPH. The results obtained in our study are in line with many studies in which the mutual effect has been monitored to increase the antioxidant capacities between herbs and metallic salts against DPPH [13][14][15][16]. In general, metallic nanoparticles display remarkable and extraordinary antioxidant impacts, among which the antioxidant effects of NiONPs have been well-documented. ...
In this study, nickel nanoparticles (NiONPs) have been successfully synthesized in an aqueous medium using the aqueous extract from the leaves of Calendula officinalis. The synthesized NiONPs were characterized using different techniques. According to the XRD analysis, the mean crystal size for NiONPs was found to be 33.17 nm. In addition, SEM images exhibited a uniform spherical morphology with a size of 60.39 nm for the NiONPs. The anti-esophageal carcinoma potentials of C. officinalis leaf aqueous extract, and NiONPs were assessed by the MTT assay against distal esophageal adenocarcinoma (FLO-1), gastroesophageal junction adenocarcinoma (ESO26), human caucasian esophageal carcinoma (OE33), and human esophageal squamous cell carcinoma (KYSE-270) cell lines. This study showed that NiONPs had excellent cell death and anti-cancer effects against esophageal carcinoma cell lines. The IC50 of NiONPs were 380, 263, 229, and 251 µg/mL against FLO-1, ESO26, OE33, and KYSE-270 cell lines, respectively. Among the studied cell lines, the best anti-esophageal cancer activities of NiONPs were gained in the cell line of OE33. The antioxidant activity of NiONPs was investigated using the DPPH test. In the antioxidant test, NiONPs prevented the oxidation of 50% of the DPPH molecules at a concentration of 204 µg/mL. Considering the obtained results, it can be suggested that NiONPs may be administrated as a chemotherapeutic supplement/drug to treat esophageal carcinoma.
