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Cupric reducing antioxidant capacity of the hydroalcoholic root extract of Diplotaenia turcica, BHT and BHA, values are expressed as means ± S.D. of three paralel measurements.
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Diplotaenia turcica is widely utilized in conventional treatment in the east of Turkey. Due to the insufficient data on the safety profile, the acute and sub acute toxicity of Diplotaenia turcica was determined. Furthermore, total phenolic content, flavonoid content and antioxidant activity of the extract were determined. The acute toxicity of hydr...
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Context 1
... other liver and kidney function parameters tested were non significantly different in all the groups compared to control (Table 3). No change in histopathology also showed the absence of toxic effects on vital organ examined and supported the biochemical and hematological results (Fig 3-4-5). ...
Citations
... The oral acute toxicity test showed that the extract of Caralluma dalzielii at 5000 mg/kg caused no mortality or any visible sign of toxicity in mice, suggesting that it may be safe (Ya'u et al., 2013;Ö zdek et al., 2018). This is in conformity with our earlier reports on the safety of the plant in rats (Ugwah-Oguejiofor et al., 2019). ...
Ethnopharmacological Relevance: Caralluma dalzielii belongs to the family of Asclepiadiaceae. It is a succulent perennial plant widely distributed across the Sahel region from Senegal to Northwest Nigeria. Extracts from Caralluma dalzielii are used traditionally in the treatment of pain, infertility, epilepsy and other neurological diseases. Aim of the study: The aim of the present study was to evaluate the anticonvulsant effects of aqueous extracts of aerial parts of Caralluma dalzielii using various experimental models. Materials and methods: The anticonvulsant effect of the plant extract (250, 500 and 1000 mg/kg p.o) were assessed using maximal electroshock (MES) model in chicks, pentylenetetrazole (PTZ) and strychnine (STN)-induced seizures models in mice. Diazepam-induced sleep in chicks was used to study the sedative property of the plant. In the anticonvulsant and sedative property studies, the onset and duration of seizures and sleep were recorded respectively. The oral acute toxicity study was assessed. The extract was screened preliminarily for its phytochemicals and was further analysed using Gas chromatography-mass spectrometry (GC–MS) to identify its major chemical components. Results: The oral acute toxicity studies showed that the LD50 was greater than 5000 mg/kg in mice. Pre-treatment of mice with the aqueous extract of Caralluma dalzielii produced a dose-dependent protection against PTZ-induced seizures and mortality with 100% protection at the highest dose of the extract. The extract also significantly increased the latency/onset of myoclonic jerks and reduced the duration of tonic seizures dose dependently. In STN-induced seizure, the extract had no protection against seizure induction similar to diazepam the positive control. However, it significantly (p
... Leaf parts were removed, washed and left to dry. After drying at room temperature for 15 minutes, the grinding process was performed [22]. For the synthesis of nanoparticles, Gurunathan et al. [23] method was used in a modified form. ...
Metal nanoparticles have been intensely researched and developed in recent years due to their superior properties. There is a growing interest in economical and environmentally friendly techniques. Cu metal is preferred in research studies due to its cheapness and effects on human health (such as production of blood cells, oxidation and reduction). In recent years, the use of plant extracts in nanoparticle synthesis has become quite popular. In this study, we aimed to investigate the antioxidant capacity and protective properties of Cu nanoparticles obtained by using Diplotaenia turcica plant against damage to pBR322 plasmid DNA. In addition, we investigated the antimicrobial effect of Cu nanoparticles against Staphylococcus aureus ATTC 29213, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 10876, Escherichia coli ATCC 25952, Candida albicans ATCC 25952. Diplotaenia turcica plant characterization with Cu metal (Cu NPs/Dt) ultraviolet and visible light absorption spectroscopy (UV-vis), fourier-transformed infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive X-ray (EDX) analysis. When looking at the antioxidant activity analysis, it is understood that it is a powerful antioxidant. It was determined that Cu NPs/Dt have significant antimicrobial activity. Its effect against some pathogens was found to be stronger than the positive control antibiotic. It was determined by the obtained DNA images that there is a potential to prevent breaks that may occur in DNA depending on the concentration.
Developing defense mechanisms against antibiotics and derivatives of microorganisms that cause infection has led to the search for new antibiotics. Many antibiotics have been isolated from plants so far. Therefore, scientists have made numerous studies on plants for the discovery of new antibiotics and continue to do so. In this study, the antimicrobial effect of extracts of Diplotaenia turcica was investigated, plants were collected from Van Çatak district. The roots and topsoil parts of Diplotaenia turcica were separated and extracted. The samples were dissolved in alcohol and ether. The pathogens used in this study were Escherichia coli ATCC 25952, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Candida albicans ATTC 90028. The effects of extracts on pathogens were determined using disc diffusion method. When the zones of plant extracts were examined, it was seen that the extract obtained from root parts of Diplotaenia turcica showed the highest inhibitory effect against Bacillus subtilis and the extract obtained from the topsoil parts of Diplotaenia turcica showed the highest inhibitory effect against Escherichia coli. Van İlinde Yetişen Diplotaenia turcica Bitkisinin Antimikrobiyal Etkisinin Araştırılması Özet Enfeksiyon sebebi olan mikroorganizmaların antibiyotik ve türevlerine karşı savunma mekanizmaları geliştirmeleri, yeni antibiyotiklerin araştırılmasına yol açmıştır. Bitkilerden şimdiye kadar birçok antibiyotik izole edilmiştir. Bundan dolayı bilim adamları, yeni antibiyotiklerin keşfi için bitkiler üzerinde sayısız çalışma yapmış ve yapmaya devam etmektedirler. Bu bağlamda mevcut çalışmamızda Van Çatak ilçesinden toplanan endemik bir tür olan Diplotaenia turcica bitkisinden elde edilen ekstraktların antimikrobiyal etkisi araştırılmıştır. Diplotaenia turcica bitkisinin kök ve toprak üstü kısımları ayrılarak ekstrakt haline getirilmiştir. Numunelerin alkol ve eter içerisinde çözünmesi sağlanmıştır. Çalışmada kullanılan patojenler Escherichia coli ATCC 25952, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Candida albicans ATTC 90028 olarak belirlenmiştir. Disk difüzyon metodu kullanılarak ekstraktların patojenler üzerine etkileri tespit edilmiştir. Bitki ekstraktlarının oluşturduğu zonlar incelendiğinde Diplotaenia turcica bitkisinin kök kısımlarından elde edilen ekstraktın Bacillus subtilis'e ve gövde kısımlarından alınan ekstraktın Escherichia coli'ye karşı en yüksek inhibitör etkiyi gösterdiği görülmüştür.
We have studied the antioxidant activity and analyzed active substances of the aqueous and methanol extracts of endemic species Thecocarpus carvifolius Hedge and Lamond. The antioxidant activity was identified and assessed using the DPPH, FRAP and iron chelating capacity assay. Active substances were detection, total phenolic, flavonoids and volatile compounds were analyzed. According to DPPH analyzes, the inhibition percentage was 48.57% for aqueous extract and 76.34% for methanol extract. The FRAP reduction capacity was determined as 3.32 mg/mL for aqueous extract and 11.75 mg/mL FESO4 for methanol extract. The total concentrations of phenolics and flavonoids were calculated as 64.08 – 35.29 μg/mL and 146.84 – 555.66 μg/mL in aqueous and methanol extracts, respectively. Finally, five volatile compounds were detected in methanol extracts. The results indicated that the endemic species of T. carvifolius can be evaluated as a natural antioxidant plant.
This study was conducted to determine the effects of Diplotaenia turcica root extract on sperm parameters and reproductive hormones in streptozotocin (STZ) induced diabetic rats. The study was performed on a total of 42 male rats divided into 6 groups of equal numbers. Single-dose physiological saline was administrated intraperitoneally to Group 1. STZ 45 mg/kg was administered intraperitoneally to Group 2. Diplotaenia turcica root extract 100 mg/kg was administrated through gastric gavage for 28 days to Group 3. Diplotaenia turcica root extract 200 mg/kg was administrated through gastric gavage for 28 days to Group 4. STZ 45 mg/kg administrated intraperitoneally to Group 5. In addition, Diplotaenia turcica root extract 100 mg/kg was administrated through gastric gavage for 28 days. STZ 45 mg/kg administrated intraperitoneally to Group 6. Also, Diplotaenia turcica root extract 200 mg/kg was administrated through gastric gavage for 28 days. Reproductive hormones and sperm parameters were analyzed. In Group 2, it was determined that sperm motility and density and reproductive hormone values were significantly lower when compared to group 1, and the abnormal sperm rate was significantly higher when compared to Group 1. Testosterone levels in Groups 3 and 4 were significantly higher than Groups 2, 5, and 6. It was determined that the 100 mg/kg dose of Diplotaenia turcica root extract, which is given also to the diabetic group, creates an improvement in sperm parameters and the hormone testosterone, but the 200 mg/kg dose does not have the same effect. As a result, we can recommend the 100 mg/kg dose of Diplotaenia turcica root extract in patients with diabetes.
Almond is a nut that is consumed abundantly all over the world. In Turkey, almonds are grown in most parts of the Turkey and the consumption of nuts has become an essential part of healthy life. Almond contain powerful nutrients, which may be related to their fatty acid composition. Therefore, the aim of present study was to investigate the antioxidant potentials and the protective activity of ethanol extraction of Amygdalus trichamygdalus fruit parts (Hull and Drupe) against the lipid peroxidation, oxidative DNA damage and protein oxidation using soxhlet ethanol extraction. To the best of our knowledge, our study can be evaluated as the first report. The total phenolic content was found to be equivalent to 28.16 ± 9.990 μg/g gallic acid extract and the total flavonoid content was equivalent to 8.866 ± 0.208 μg/g quercetin extract. The present study shows that A. trichamygdalus extract have good protective activity against lipid peroxidation in the FeCl2–H2O2 system. The extract also showed a strong DPPH radical scavenging ability in a concentration-dependent manner. At 2000 μg/ml, the DPPH radical scavenging activity was found to be 84.47 ± 0.147%. The protein oxidation was also inhibited by A. trichamygdalus extract. At 2000 μg/ml, A. trichamygdalus extract protected protein oxidation by 76.27 ± 1.14%. In addition, the DNA oxidation damage against OH radicals resulting from H2O2 photolysis was inhibited concentration dependently and DNA damage was suppressed by 50.26 ± 2.6% at 1000 μg/ml concentration. The results suggest that A. trichamygdalus fruit extract have antioxidant activity and protect lipid, protein and DNA against oxidative damage. Therefore, the extract may be useful in the food industry as natural antioxidants.
Background
Diplotaenia turcica has been used traditionally to diabetes treatment. In this study, the effects of D. turcica root extract (DT) on diabetes mellitus induced by streptozotocin (STZ) were investigated.
Materials and methods
In this study, 78 male rats were used, rats were divided into 9 groups randomly. In diabetic groups, STZ was given a single dose of 45 mg/kg by intraperitoneally. DT (50, 100 and 200 mg/kg) and glibenclamide (5 mg/kg) were given by orally. Blood and pancreas tissue samples were taken for biochemical and pathological tests.
Results
It was found that glucose levels decreased, and insulin levels increased in the treatment groups compared with the diabetes group. In addition, only in 200 mg/kg DT dose group was found to decrease HbA1c levels. Pancreatic tissue analysis showed that MDA levels decreased and GSH levels and CAT, SOD, GSH-Px and GSH-R activities increased in diabetic rats treated with DT. Histopathological and immunohistochemical examinations of the pancreas showed significant improvements in the treatment with DT.
Conclusion
These results clearly show the antioxidant property of DT. The findings of this study showed that increased doses of DT may have a therapeutic effect on STZ-induced pancreatic damage.
