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Culture of cyanobacteria: A. in the test tube as a culture collection, B. in Erlenmeyer flasks for fatty acid analysis
Source publication
The species diversity of thermophilic cyanobacteria from a geothermal area were poorly studied, especially in Indonesia. This study was aimed to explore the diversity of culturable thermophilic cyanobacteria from geysers in Cisolok Hot spring (90-100 °C, pH 7.0-8.0), West Java. Twelve cyanobacterial strains were isolated from this study. Based on m...
Contexts in source publication
Context 1
... intensity of light given was 3000-5000 lux, with photoperiodicity 12L/12D regulated by a timer. Incubation temperature which used were 20 ± 5 o C; 30 ± 5 o C; and 50 ± 5 o C. Cyanobacteria cultures in test tubes and Erlenmeyer flasks were shown at Fig.1. ...
Context 2
... addition, monounsaturated fatty acids 18:1w9c[(Z)-octadec-9-enoic acid, n-9/Oleic acid/ C17H33CO2H] ranged from 1.43 to 35.78% owned by most strains, especially strains Leptolyngbia (HS-16, HS-27, HS-28, HS-30A, HS-31A, HS-33, HS-34, HS- 36, HS-39, HS-40B, HS-40C, HS-41, HS-42, HS-49), Westiellopsis (HS-10), and Mastigocladus (HS-40A, HS- 46). The polyunsaturated fatty acids/ Di-unsaturated 18:2w6,9c[(6Z,9z)-octadec-6,9-dienoic acid, n- 6/Linoleic acid/ C17H33CO2H] were owned by the majority of genus Leptolyngbya (HS-28, HS-30A, HS- 31A, HS-33, HS-36, HS-39, HS-40B, HS-40C, HS-42, HS-49), the genus Westiellopsis (HS-10), the genus Mastigocladus (HS-40A), and the genus Nostoc (HS-5, HS-20) ranged from 2.56 to 29.62%. ...
Citations
... The studies on geothermal springs in Eastern Algeria reported the occurrence of nineteen cyanobacterial morphotypes with the dominance of the genus Leptolyngbya (Amarouche-Yala and Benouadah, 2014). Prihantini et al. (2018) reported twelve culturable strains with three novel taxa of thermophilic cyanobacteria from geysers of Cisolok Hot spring in Indonesia. Cyanobacterial diversity in highly saline environments could also be higher, where hypersaline lagoons such as Araruama's lagoons in Brazil exhibited a high cyanobacterial diversity with 36 morphospecies belonging to 22 genera (Ramos et al., 2017). ...
... Many previous studies on extreme environments also reported the frequent occurrence of Leptolyngbya. Leptolyngbya was recorded in all sampling points of geysers in Cisolok Hot spring in West Java, Indonesia, during the characterization of culturable cyanobacteria (Prihantini et al., 2018). The study on cyanobacterial composition in Eynal (Simav) hot spring in Kütahya, Turkey also recorded Leptolyngbya as the most abundant filamentous morphotype (YilmazCankilic, 2016). ...
... Not only in thermal springs but also in saline environments and mangroves, Leptolyngbya was one of the most abundant genera (Rigonato et al., 2012;Ramos et al., 2017). Easy morphological characterization and the ability to grow easily under laboratory conditions could be reasons for the higher detectability of this genus during diversity evaluations (Prihantini et al., 2018). Other than Leptolyngbya, Geitelerinema, Oscillatoria, and the unidentified genus of Chroococcales were recorded in all four ecosystems. ...
... Images of Leptolyngbya sp. were captured in sample SD-1. Prihantini et al. 54 reported that Leptolyngbya is one of the cyanobacteria that grow well in culturing and they are fine filaments that are long and thin, with 0.5 to 3.2 μm dimension. They have heterocysts 54 . ...
... Prihantini et al. 54 reported that Leptolyngbya is one of the cyanobacteria that grow well in culturing and they are fine filaments that are long and thin, with 0.5 to 3.2 μm dimension. They have heterocysts 54 . ...
... Furthermore, the PCR-based approach was conducted as a complementary method to confirm the flow cytometric analysis results. Identification using molecular characters was needed to identify cyanobacteria that could not be identified by morphological characters 54 . About seven phyla were amplified, of which the cyanobacteria had the largest fractions in most of the samples. ...
Globally, the occurrence of cyanobacteria in water currently remains an important subject as they produce cyanotoxins that pose threat to human health. Studies on the contamination of maize meals during mill grinding processes using cyanobacteria-contaminated water have not been conducted. The present study aimed to assess the diversity of cyanobacteria in the samples (process water, uncooked maize meal, and cooked maize meal (porridge)). Polymerized Chain Reaction (PCR) and Advanced digital flow cytometry (FlowCAM) were used to detect and identify cyanobacterial species available in these samples. 16S Primers (forward and reverse) tailed with Universal Sequences were used for amplification and sequencing of full-length 16S rRNA genes from cyanobacteria found in all samples. Cyanobacterial species from order Nostocales, Pseudanabaenales, Oscillatoriales Chroococcales, Synechococcales, and unclassified cyanobacterial order, some of which have the potential to produce cyanotoxins were amplified and identified in process water, raw maize meal and porridge samples using PCR. Images of the genus Microcystis, Phormidium, and Leptolyngbya were captured in process water samples using FlowCAM. These findings show the presence of cyanobacteria species in process water used for maize meal and the absence in cooked maize meal. The presence of cyanobacteria in process water is likely another route of human exposure to cyanotoxins.
