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... grinding time was 30 s per bird, so that the resulting mass could stay on the desired consistency and be mixed for the experiment. After grinding, the whole mass was again mixed in a container, forming the substrate as shown in Figure 1. For the conduct and development of the research, 9 units of batch biodigesters were used, each with a total capacity of 8 L. This model was chosen because it is a most recommended system for treating waste which is removed less frequently, or in systems where the daily cleaning is not practiced.The batch biodigesters are composed of three distinct pairs, one of them being the container with the material in fermentation, the gasometer and the compartment is to form the water-seal. ...

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... Biogas produced was allowed to bubble through a 3 M NaOH solution so that mostly methane, the volume of which was equivalent to the volume of alkali displaced, was collected and recorded daily [33]. The experiments were concluded when daily gas production became <1% of cumulative volume for three consecutive days [34]. Equation (5) was used to calculate the BMP as the average volume of methane produced by the substrate-inoculum systems less that from the inoculum only systems and reported as Nml CH 4 (gVS) À1 at STP condition [35]. ...
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