Figure - available from: Frontiers in Genetics
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Conjoint analysis of MeRIP-seq and RNA-seq data. (A) Volcano plots showing the differentially expressed genes in villous tissues of SA group compared with normal group. (B) Heatmap plots showing the differentially expressed genes in villous tissues of SA group compared with normal group. (C) Four-quadrant plots showing the distribution of genes with significant changes in both the m⁶A modification and mRNA levels. (D) The top 5 GO terms of genes with significant changes in both the m⁶A modification and mRNA levels.
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A growing number of studies have demonstrated that N6 methyladenine (m⁶A) acts as an important role in the pathogenesis of reproductive diseases. Therefore, it is essential to profile the genome-wide m⁶A modifications such as in spontaneous abortion. In this study, due to the trace of human villi during early pregnancy, we performed high-throughput...
Citations
... Abnormal RNA methylation plays a role in abnormal embryo implantation and may also lead to spontaneous abortion. High-throughput sequencing of villous tissue from the spontaneous abortion group and the normal group during early pregnancy, based on combined analysis of meRIP-seq and RNA-seq data, revealed that genes with differential m6A methylation were primarily related to the Wnt signaling pathway, phosphatase activity regulation, protein phosphatase inhibition activity, and transcriptional inhibition activity (198). The expression levels of ALKBH5 and CYR61 were negatively correlated in the villous tissue of the recurrent abortion group, which is related to the pathogenesis of recurrent abortion (169). ...
The maternal-fetal interface is defined as the interface between maternal tissue and sections of the fetus in close contact. RNA methylation modifications are the most frequent kind of RNA alterations. It is effective throughout both normal and pathological implantation and placentation during pregnancy. By influencing early embryo development, embryo implantation, endometrium receptivity, immune microenvironment, as well as some implantation and placentation-related disorders like miscarriage and preeclampsia, it is essential for the establishment of the maternal-fetal interface. Our review focuses on the role of dynamic RNA methylation at the maternal-fetal interface, which has received little attention thus far. It has given the mechanistic underpinnings for both normal and abnormal implantation and placentation and could eventually provide an entirely novel approach to treating related complications.
... RNA m 6 A methylomes across fetus and adult tissues depicted the dynamic m 6 A methylation across different tissue types, covering both broadly or tissue-specifically m 6 A sites (Xiao et al., 2019;Zhang et al., 2020a). In agreement with previous studies, the m 6 A modifications in placental tissues are mostly enriched around stop codons, with a consensus motif of RRACH, indicating the high conservation of m 6 A in human placentas (Taniguchi et al., 2020;She et al., 2022). However, abundant tissue-differential m 6 A peaks are identified in placenta which is higher than that in brain, heart, kidney and other tissues (Xiao et al., 2019). ...
... Epigenetics also participates in the pathogenesis, while the detailed mechanism has not been fully understood. Based on MeRIP-seq data of villous tissues from SA, m 6 A peaks are still mainly located in the codding region and near the stop codon, with a consensus sequence of RRACH (She et al., 2022). Differential m 6 A-modified genes are mainly involved in the Hippo and Wnt signal pathways, phosphatase activity regulation and transcription inhibitor activity (She et al., 2022). ...
... Based on MeRIP-seq data of villous tissues from SA, m 6 A peaks are still mainly located in the codding region and near the stop codon, with a consensus sequence of RRACH (She et al., 2022). Differential m 6 A-modified genes are mainly involved in the Hippo and Wnt signal pathways, phosphatase activity regulation and transcription inhibitor activity (She et al., 2022). At the maternal-fetal interface, FTO, IGF2BP1 and IGF2BP2 are decreased in abortion tissues, with a decreased level of FTObound HLA-G, VEGFR and MMP9 mRNA (Qiu et al., 2021). ...
As a medium among pregnant women, environment and fetus, placenta owns powerful and delicate epigenetic processes to regulate gene expression and maintain cellular homeostasis. N6-methyladenosine (m⁶A) is the most prevalent modification that determines the fate of RNA, and its dynamic reversibility indicates that m⁶A may serve as a sensitive responder to environmental stimuli. Emerging evidence suggests that m⁶A modifications play an essential role in placental development and maternal-fetal crosstalk, and are closely related to gestational diseases. Herein, we summarized the latest techniques for m⁶A sequencing and highlighted current advances of m⁶A modifications in maternal-fetal crosstalk and the underlying mechanisms in gestational diseases. Therefore, proper m⁶A modifications are important in placental development, but its disturbance mainly caused by various environmental factors can lead to abnormal placentation and function with possible consequences of gestational diseases, fetal growth and disease susceptibility in adulthood.
