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Conjoint analysis of DEGs and DMGs. (A) Nine-quadrant plot of DEGs and DMGs. The abscissa represents DMGs and the ordinate represents DEGs. Red dots represent genes up-regulated at the mRNA and m 6 A levels, yellow dots represent genes up-regulated at the mRNA level and down-regulated at the m 6 A level, and green dots represent genes down-regulated at both the mRNA and m 6 A levels. (B) Venn diagram of DEGs and DMGs. The p-value is the hypergeometric distribution, and all genes detected by sequencing was set as the total number. (C) GO enrichment analysis of genes with altered mRNA level and m 6 A modification. (D) KEGG enrichment analysis of genes with altered mRNA level and m 6 A modification.
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Background:
N6-methyladenosine (m6A) participates in diverse physiological processes and contributes to many pathological conditions. Epithelial-mesenchymal transition (EMT) of retinal pigmental epithelial (RPE) cells plays an essential role in retinal-related diseases, and transforming growth factor β2 (TGF-β2) is known to induce EMT in vitro. Ho...
Contexts in source publication
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... analysis of DEGs and DMGs was performed to identify genes with altered mRNA and m 6 A modification. As shown in Fig. 4A, one gene showed up-regulated mRNA and hypermethylated peaks, 215 genes had upregulated mRNA and hypomethylated peaks, and 75 genes had down-regulated mRNA and hypomethylated peaks. Venn diagram analysis showed that 290 genes were altered at the m 6 A and RNA levels (Fig. 4B, Supplementary Ta- ble 6). Comparing Fig. 4A,B, the CCNF gene ...
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... to identify genes with altered mRNA and m 6 A modification. As shown in Fig. 4A, one gene showed up-regulated mRNA and hypermethylated peaks, 215 genes had upregulated mRNA and hypomethylated peaks, and 75 genes had down-regulated mRNA and hypomethylated peaks. Venn diagram analysis showed that 290 genes were altered at the m 6 A and RNA levels (Fig. 4B, Supplementary Ta- ble 6). Comparing Fig. 4A,B, the CCNF gene was found to be up-regulated at the transcriptional level, but contained both hypermethylated and hypomethylated ...
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... diagram analysis showed that 290 genes were altered at the m 6 A and RNA levels (Fig. 4B, Supplementary Ta- ble 6). Comparing Fig. 4A,B, the CCNF gene was found to be up-regulated at the transcriptional level, but contained both hypermethylated and hypomethylated peaks. ...
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... enrichment analysis revealed the conjoint genes were associated with cell division, the cell cycle, chromosome segregation, and the microtubule cytoskeleton (Fig. 4C). KEGG enrichment analysis showed the conjoint genes were related to the cell cycle, apoptosis, focal adhesion, ECM-receptor interaction, protein digestion and absorption, and the signaling pathways for AGE-RAGE, PI3K-Akt, cGMP-PKG, and p53 (Fig. ...
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... with cell division, the cell cycle, chromosome segregation, and the microtubule cytoskeleton (Fig. 4C). KEGG enrichment analysis showed the conjoint genes were related to the cell cycle, apoptosis, focal adhesion, ECM-receptor interaction, protein digestion and absorption, and the signaling pathways for AGE-RAGE, PI3K-Akt, cGMP-PKG, and p53 (Fig. ...
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... analysis of DMGs and DEGs revealed that 290 genes were simultaneously altered at the m 6 A and RNA levels. These conjoint genes were enriched in ECMreceptor interactions, AGE-RAGE signaling, PI3K-Akt signaling, cGMP-PKG signaling, and the p53 signaling pathway (Fig. 4B,D). ECM-receptor interactions have pro- found effects on major cellular programs including growth, differentiation, migration and survival, and have been associated with the EMT process in RPE cells [48]. AGE-RAGE signaling enhances NF-kB signaling, stimulates the generation of reactive oxygen species, and accelerates the EMT process ...
