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The present study investigates the effect of polyphenolic extract of Capparis spinosa L. Leaves on acute toxicity in female rats and anti-bacterial in vitro. The working of this thesis involves the following terms: Analytical study involved extraction, isolation, and identification of polyphenolic content of Capparis spinosa L. extract. The UV-Vis...
Citations
... Other studies mentioned that the root extract could be an analgesic and antibacterial due to various traditional medicines' excellent economic and medicinal properties in many Asian and European countries such as Iran, China, Greece and Arabia [34,35] . According to Syrian tradition, only powdered roots or "pastes" obtained by mixing powder with water are widely used for oral/topical application on painful areas [36] . ...
... Interestingly, C. spinosa is safe and has no scientific evidence of side effects or toxicity. Based on the composition of the active ingredient of C. spinosa, it is believed that it may have potential protective effects against oxidative stress, genotoxicity and cytotoxicity in animal models [34,75,76] . ...
This study aimed to estimate the ant-arthritic activity of Capparis spinosa L. roots extract in complete Freund’s adjuvant (CFA)-induced arthritis mice by assessment of paw thickness, serological detection of inflammatory markers [rheumatoid factor (RF) and tumor necrosis factor-alpha (TNF-)] using ELISA, and histopathology. In total, 30 male mice were selected, prepared, and divided equally into five groups, including 2 control (NC and PC)and 3 experimental: EG1 (arthritis mice treated total effective dose of extract), EG2(arthritis mice treated total effective dose of Diclofenac Sodium), and EG3 (arthritis mice treated a half effective dose of each the extract and Diclofenac). Clinically, the extract administration lowered the paw thickness from day 7th onwards, the 21st day of study, while the extract administration lowered the paw thickness in EG1 from day 7th onwards compared to EG2 and EG3. For hematology, significant decreases were reported in RBCs, HCT, Hb, WBCs and neutrophils due to arthritis, and significant amelioration was seen obviously in mice of EG1 as a result of therapy. Significant increases in platelets, lymphocytes and monocytes were observed in PC and experimentally groups, significantly improving the values of treated groups due to therapy, in particular, EG1. All study groups ' values of MCV, MCH, MCHC, basophils and eosinophils have differed insignificantly. Concerning RF, the highest significant value was reported in PC while the lowest in NC, but without significant differences between EG1, EG2 and EG3 values. For TNF-, elevation had been recorded in PC mice, whereas reduction in NC, EG1 and EG3. Also, the value of EG2 was significantly higher than recorded in mice of EG1 and EG3. Concerning histology, the findings of EG1 revealed a significant amelioration compared to other groups. In conclusion, C. spinosa L. root extract demonstrated anti-inflammatory and anti-arthritic activity. Furthermore, studies to detect the therapeutic effects of the root extract on other systemic or local diseases are needed. Keywords: Paw thickness; Rheumatoid factor; Inflammatory markers; Diclofenac Sodium; Iraq
... As a result, photoreactions facilitated by UCNPs on UCNP surfaces are often more efficient than photoreactions facilitated by UCNPs in solution, because photosensitive compounds are located further away from UCNPs [113]. Therefore, Converting IR to UV for releasing drugs or producing ROS can be a hopeful candidate for cancer therapy [114][115][116]. By considering this idea, NaYF 4 :Yb,Tm was synthesized by Yeh group. ...
... So the Clinical and Laboratory Standards Institute (CLSI) recommends establishing population-specific reference ranges that take into account the varying ages and ethnicities of the general population as well as the various genders and racial/ethnic groups (8,9). Experimental preparation requires that patients' laboratory results be matched to the relevant reference intervals (RIs) before any clinical judgments can be made on their care (10,11). Risk factors for illness, including hematological and immunological risk variables (12), are used to identify people at risk for illness and evaluate their immune status, disease progression, and response to therapy (12). ...
... Oudah et al.,(53) reported the highest activity of extracted polyphenolic for C. spinosa against E. coli and showed 12mm inhibition zone. Hameed et al.,(54) noticed that the majority of phenols, regardless of their source from the plant, have shown efficacy against bacteria, and this indicates an increase in the concentration of the active components in the reproductive partsConc. ...
This study was conducted to evaluate the synergistic effect between Capparis Spinosa fruits methanolic extract and ciprofloxacin against resistant E. Coli O157:H7, in varying concentration. This experiment was carried out through the ultrasonic alcoholic extraction of C. Spinosa fruits, and an extraction ratio of 24% was obtained. The extract showed pronounced concentration dependent antibacterial activity. The susceptibility study revealed that E. Coli O157:H7 was sensitive to C. Spinosa fruits. The findings of the present study indicate that the use of pronounced C. Spinosa fruits extract may have the perfect to be choice in clinical control. The concentration of C. spinosa fruits extract increase the activity of inhibition, increase as well when used in combination with ciprofloxacin (half concentration from each other). In result appeared synergism effect between plant extract and ciprofloxacin and may make bacteria lost their resistance to antibiotics by this synergism phenomenon. The result of MIC (6400 µg/ ml), MBC (12800 µg/ ml) and for C. spinose fruits extract while ciprofloxacin MIC (12.5 µg/ ml) and MBC (25 µg/ ml). MICs of CIP/ C. spinosa fruits extract combinations against E. coli O157:H7 isolate was 1.562/ 1600 μg/ml. Value of minimum bactericidal concentration (MBC) of the synergistic combinations (CIP/ C. spinosa fruits extract) was 3.124/ 3200 μg/ml. According to obtained results fractional inhibitory concentration (FIC) value of (CIP/ C. spinosa fruits extract 1.562/ 1600 μg/ml) was (0.375) less than 0.5 indicates synergistic effect of interaction.
... As detected in this study, anti-inflammatory effect of C. spinosa L. roots extract was found by other studies in root [30,31] , fruits [32] , buds [30] and leaves [33] . Other studies mentioned that the root extract can be used as an analgesic and antibacterial due to the excellent economic and medicinal properties with various traditional medicines in many Asian and European countries such as Iran, China, Greece and Arabia [34,35] . According to Syrian tradition, only powdered roots or "pastes" obtained by mixing powder with water are widely used for oral / topical application on painful areas [36] . ...
... This could be explained by the fact that this extract is rich by glucosinolates (glucosinolates, glucosides, myrosins, glucosinolates), flavonoids, phenolic acids and alkaloidswhich provide health benefits from a variety of biological properties that have different biological activities including antioxidant, anti-inflammatory, anti-cancer, antibacterial, anti-mutagenic, and anti-diabetic properties [71][72][73][74] . Interestingly, C. spinosa is safe and has no scientific evidence of side effects or toxicity; and based on the composition of the active ingredient of C. spinosa, it is believed that it may have potential protective effects against oxidative stress, genotoxicity and cytotoxicity in animal models [34,75,76] . ...
This study aimed to estimate ant-arthritic activity of Capparis spinosa L. roots extract in complete
Freund’s adjuvant (CFA)-induced arthritis mice by assessment of paw thickness, serological detection of inflammatory markers [rheumatoid factor (RF) and tumor necrosis factor-alpha (TNF-)] using ELISA, and
histopathology. Totally, 30 male mice were selected, prepared, and divided equally to five groups including 2
control (NC and PC)and 3 experimental; EG1 (arthritis mice treated full effective dose of extract),
EG2(arthritis mice treated full effective dose of Diclofenac Sodium), and EG3 (arthritis mice treated a half
effective dose of each the extract and Diclofenac). Clinically, the extract administration lowered the paw
thickness from day 7th onwards the 21st day of study; while, the extract administration have lowered the paw
thickness in EG1 from day 7th onwards as compared to EG2 and EG3. For hematology, significant decreases
were reported in RBCs, HCT, Hb, WBCs and neutrophils due to arthritis, and significant amelioration was
seen obviously in mice of EG1 as a result of therapy. Significant increases in values of platelets, lymphocytes
and monocytes were observed in PC and experimentally groups, with significant improving for values of
treated groups due to therapy, in particular, EG1. Values of MCV, MCH, MCHC, basophils and eosinophils of
all study groups were differed insignificantly. Concerning RF, highest significant value was reported in PC
while the lowest in NC, but without significance differences between values of EG1, EG2 and EG3. For
TNF-, elevation had recorded in mice of PC; whereas, reduction in NC, EG1 and EG3. Also, value of EG2
was significantly higher than recorded in mice of EG1 and EG3. Concerning histology, the findings of EG1
were revealed a significant amiolration when compared to other groups. In conclussion, C. spinosa L. roots
extract demonstrated anti-inflammatory and anti-arthritic activity. Furthermore studies to detect therapeutic
effects of the roots extract on other systemic or local diseases are needed.
... In our study, caffeic acid was not detected in calluses in any medium, including the M1 medium (data not shown). According to Oudah et al. (2019) the amount of caffeic acid in caper leaves was 73.542 μg/ml, but Rezzan et al. (2013) did not find any caffeic acid in the caper leaves collected from Gaziantep (Turkey). Antognoni et al. (2008) reported that α-tocopherol production in calli of Amaranthus caudatus and Chenopodium species was approximately 40 times lower than the tocopherol content in the leaves and other plant organs. ...
The effect of strigolactones on plants, which has been recently described as a new group of plant hormones, has not been fully characterized. Capparis spinosa L. callus formation using synthetic strigolactone GR24 (0.1 and 0.2 μM) alone or combined with 1-naphthalene acetic acid (NAA) (10.74 μM) and 6-benzylaminopurine (BAP) (4.44 μM) and its effect on phenolic compounds production were evaluated. Compared to the other media, the media with 10.74 μM NAA + 4.44 μM BAP + 0.1 μM GR24 or 10.74 μM NAA + 4.44 μM BAP gave the highest callus formation. The highest rutin, quercetin and chlorogenic acid content were found in a medium with 10.74 μM NAA + 4.44 μM BAP + 0.1 μM GR24. Aromatic compounds in caper calluses were grouped as sulfur compounds (66.97–87.53%), aldehydes (4.88–7.90%), ketones (0.34–19.3%), hydrocarbons and derivatives (0.56–5.8%), alcohols (1.62–6.08%), others (0.61–2.37%) and their amounts varied at various hormone applications. When 0.2 μM GR24 was applied solitarily, the total sulfur compound in callus samples was 87.53% and the dominant compound was found to be methyl isothiocyanate. These results suggested that GR24 may be effective in the accumulation of chlorogenic acid, rutin and quercetin phenolics in caper callus culture.
... Animals were fed on standard rat pellet and tap water. The standard pellet contains wheat 66.6%, soya 25.6%, and sun flower oil 4.4%, lime stone 1.5%, salt 0.63%, methionine 0.158%, choline chloride 0.062% and trace elements 0.05% (Oudah et al., 2019). ...
The current study was conducted to find out the effect of sesame oil on white female rats by studying some biochemical parameters that included vitamin D, parathyroid hormone and calcium ion. The study included 35 animals divided into two main groups divided into groups, each group included 5 animals dosed with different doses 0.5'0.8' 1 ml. The period of dosing was for a month between one day and one day. After the end of the experiment, the animals were sacrificed and the serum was obtained. A significant increase in the volume of vitamin D (13.21±0.312, 16.01± 0.51, 19.51±0.78) (ng/dl) respectively compared with control group (9.739±0.096) (ng/dl) and a decrease in parathyroid hormone (0.101± 0. 001, 0.086± 0. 002 , 0.074± 0.002) (ng/dl) respectively compared with control group (0. 112± 0.002) (ng/dl), which caused an increase in calcium ion (9.85±0.206, 10.948±0.178, 12.62±0.158) mg/dL respectively compared with control group (8.7±0.063) mg/dL, was shown.
... Oudah et al.,(53) reported the highest activity of extracted polyphenolic for C. spinosa against E. coli and showed 12mm inhibition zone. Hameed et al.,(54) noticed that the majority of phenols, regardless of their source from the plant, have shown efficacy against bacteria, and this indicates an increase in the concentration of the active components in the reproductive partsConc. ...
This study was conducted to evaluate the synergistic effect between Capparis Spinosa fruits methanolic extract and ciprofloxacin against resistant E. Coli O157:H7, in varying concentration. This experiment was carried out through the ultrasonic alcoholic extraction of C. Spinosa fruits, and an extraction ratio of 24% was obtained. The extract showed pronounced concentration dependent antibacterial activity. The susceptibility study revealed that E. Coli O157:H7 was sensitive to C. Spinosa fruits. The findings of the present study indicate that the use of pronounced C. Spinosa fruits extract may have the perfect to be choice in clinical control. The concentration of C. spinosa fruits extract increase the activity of inhibition, increase as well when used in combination with ciprofloxacin (half concentration from each other). In result appeared synergism effect between plant extract and ciprofloxacin and may make bacteria lost their resistance to antibiotics by this synergism phenomenon. The result of MIC (6400 µg/ ml), MBC (12800 µg/ ml) and for C. spinose fruits extract while ciprofloxacin MIC (12.5 µg/ ml) and MBC (25 µg/ ml). MICs of CIP/ C. spinosa fruits extract combinations against E. coli O157:H7 isolate was 1.562/ 1600 μg/ml. Value of minimum bactericidal concentration (MBC) of the synergistic combinations (CIP/ C. spinosa fruits extract) was 3.124/ 3200 μg/ml. According to obtained results fractional inhibitory concentration (FIC) value of (CIP/ C. spinosa fruits extract 1.562/ 1600 μg/ml) was (0.375) less than 0.5 indicates synergistic effect of interaction.
... C. spinosa has been revealed to have many bioactivities, such as those of antioxidant, antifungal, antihepatotoxic, anti-inflammatory, antiallergic, and antihistaminic impacts. Also, it is considered as antidiabetic, chondroprotective, hypolipidemic, diuretic, antihypertensive, and antibacterial [10][11][12][13][14][15][16][17]. Acetic acid is a short-chain fatty acid which was reported to exert enhancement roles in the digestion and metabolism processes [18]. ...
This study aimed to determine the effects of alcoholic and aqueous extracts of caper (Capparis Spinosa) and acetic acid on serum lipid profile and proteins levels in mice. Sixty adult mice with an average weight of 24±4 g grams were divided into four groups (15 mice for each). The first group (G1) was administrated daily with an oral dose of caper alcoholic extract (200 mg/kg) for 28 days. The second group (G2) was administrated daily with an oral dose of caper aqueous extract (200 mg/kg) for 28 days. The third group (G3) was administrated with a daily dose of 10 % acetic acid (2 ml/kg) for 28 days. The fourth Group (G4) was administrated daily with distilled water for 28 days, as a control group. The levels of lipid profile parameters, blood urea, total protein, albumin, and globulin were determined. The results showed a significant reduction (P≤0.05) in cholesterol and triglyceride levels in mice that were treated with alcohol or aqueous extracts of caper compared with acetic acid-treated and control groups. On the other hands, the results showed a significant reduction (P≤0.05) of blood urea levels in mice that were treated with alcohol or aqueous extracts of caper compared with acetic acid-treated and control groups. While the results recorded non-significant differences in the levels of total protein, albumin, and globulin in the serum of mice of different treatment groups. From the results, it can be concluded that caper has protective effects via acting to improve the lipid profile and urea level in the blood of mice.
... Furthermore, L-carnitine is essential for maintaining normal lactate levels of in the cardiacmyocyte [28]. In heart failure, the pattern of energy expenditure switch to other substrate in expense of fatty acid leading to increase plasma level of fatty acid which further deteriorate cardiac performance [33].Heart failure is a multifactorial disease with a wide scope of pathophysiological mechanisms, it is believed that L-carnitine deficiency is one of the abnormalities which predispose to improper energy consumption due to impaired βoxidation of fatty acid in mitochondria [34].Accordingly, L-carnitine supplement in patient with heart failure may act to correct the derangement of mitochondrial function by increasing fatty acid utilization and improve cardiac performance to some extent [35][36][37][38][39]. ...
Background:L-carnitine deficiency is common in patients with chronic kidney disease, especially those who are in regular dialysis. L-carnitine deficiency associated with high incidence of oxidative stress, dyslipidemia and impaired cardiac performance.The present study tends to evaluate the effect of L-carnitine supplement on cardiac output in patients with chronic kidney disease on regular hemodialysis.
