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Concentration-dependent uptake of GL by Caco-2 cells (A). The cells were incubated in HBSS containing different concentrations of GL ammonium salt at 37 ºC for 90 min. Time-dependent GL uptake by Caco-2 cells (B). The cells were incubated in HBSS containing 200 mol·L −1 GL ammonium salt at 37 ºC for different times. Effects of Laminarin on GL uptake (C) and GL metabolism (D) in Caco-2 cells. The cells were incubated with GL ammonium salt (200 mol·L −1 ) and Laminarin (10, 100 and 400 mol·L −1 ) for designated times. The data are expressed as mean ± SD (n = 3)
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