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Comparison of different staining methods in the diagnosis of reticulin fibrosis in cavy liver A -haematoxylin-erythrosine saffron method B -Masson-Goldner trichrome C -silver impregnation D -haematoxylin-eosin saffron (HES) method; asterisk -collagen (mainly type I) fibres; arrowhead -reticular (type III collagen) fibres. All scale bars = 100 µm
Source publication
Introduction:
The aim of our study was to optimise an existing staining procedure: haematoxylin-eosin saffron (HES). The method follows the classical haematoxylin and eosin protocol with the addition of a staining step using natural saffron to better identify the collagen fibres.
Material and methods:
The saffron solution was obtained by dissolv...
Contexts in source publication
Context 1
... sample. In rabbit tooth samples (Fig. 2E and F), haematoxylin-erythrosine saffron did not stain the dentin. Contrary findings were made with HES, which impregnated this sample satisfactorily. HES in diagnostic applications. HES staining is also very useful in the diagnosis of reticulin fibrosis. Both type I and III collagen are clearly visible (Fig. 3D), while the structure of the cell cytoplasm is clearer than in Masson-Goldner trichrome staining (Fig. 3B). Haematoxylin-erythrosin saffron staining (Fig. 3A) applied to the same specimen of cavy liver results in lower contrast between connective and glandular (epithelial) tissue. Type III collagen is also more difficult to discern. ...
Context 2
... Contrary findings were made with HES, which impregnated this sample satisfactorily. HES in diagnostic applications. HES staining is also very useful in the diagnosis of reticulin fibrosis. Both type I and III collagen are clearly visible (Fig. 3D), while the structure of the cell cytoplasm is clearer than in Masson-Goldner trichrome staining (Fig. 3B). Haematoxylin-erythrosin saffron staining (Fig. 3A) applied to the same specimen of cavy liver results in lower contrast between connective and glandular (epithelial) tissue. Type III collagen is also more difficult to discern. Similar results to those of HES were obtained only with silver impregnation, an elaborate and specific ...
Context 3
... this sample satisfactorily. HES in diagnostic applications. HES staining is also very useful in the diagnosis of reticulin fibrosis. Both type I and III collagen are clearly visible (Fig. 3D), while the structure of the cell cytoplasm is clearer than in Masson-Goldner trichrome staining (Fig. 3B). Haematoxylin-erythrosin saffron staining (Fig. 3A) applied to the same specimen of cavy liver results in lower contrast between connective and glandular (epithelial) tissue. Type III collagen is also more difficult to discern. Similar results to those of HES were obtained only with silver impregnation, an elaborate and specific methodology designed to stain type III collagen. Fig. 1. ...
Citations
... HE and S staining, introduced by Edston and Grafti (1997) to substitute other combinations for staining specific tissues, provided impressive results. Natural saffron solution is used to identify collagen fibers, as it gives reproducible and reliable results (Ceccopieri et al., 2021). Collagen is one of the tissue components that adds strength to the organs and it protects structures, gives strength and elasticity, and helps the tissues to bear physical stress and tissue damages via its strong stiffened network. ...
In this paper, a structural analysis of the rarely encountered hydrozoan medusa
Limnocnida indica Annandale is discussed. This diploblastic, radially
symmetrical organism has a body plan typical of hydrozoan medusae. In this
study more emphasis was given to observation of the supporting components of
its anatomical structures. Detailed observation on the distribution of supportive
components including chitin, collagen, and noncellular fibers within the tissue
organization has been carried out with the application of different types of
staining methods. The neuromuscular arrangement at the rim of the umbrella and
basal part of the gastrovascular cavity and the distribution of the neurons in the
sub epithelial region of ectoderm have been analyzed both by staining and by
scanning electron microscopy studies. Supporting blocks of chitin at the base of
tentacles, along with statocysts, and ring-like deposition of chitin and collagen
on the tentacles are documented. Distribution of collagen around radial canals in
the mesoglea was found to be unique to this species. The mesoglea seems to be a
storehouse for the macromolecules chitin and collagen.
... Several other methods that can be used to evaluate sperm morphology are Eosin-nigrosin (5), AgNO3 (6) and Giemsa (7) staining. However, using these synthetic dyes can be harmful; using them can harm the environment and water sources because they prefer to decompose (8 (13), rosella (14), curcumin (15), saffron (16) and red dragon (17). ...
Examination of sperm morphology is an essential criterion for evaluating male fertility. This examination can be done by staining sperm cells with various techniques to facilitate the visualization of sperm cells. Several other methods that can be used to evaluate sperm morphology are Eosin-nigrosin, AgNO3 staining, Papanicolaou, Diff Quick and Giemsa. However, using these synthetic dyes can harm the environment and water sources because they prefer to decompose. The natural dye derived from environmentally friendly plants expected to minimize hazardous waste. This study aimed to identify and compare the potency of several natural dyes derived from purple cabbage, sappan wood and beetroot, which have not been studied in sperm studies. Sperm obtained from 30 men in the campus area and underwent 3-5 days of abstinence from intercourse in preparation for sampling. The sperm stained with natural dye, and their quality compared with WHO standard Papanicolaou. Extracts of sappan wood, purple cabbage and beetroot are made with various mordant alum, ethanol and acetic acid compositions. The study's results showed that the presence of mordant increased the staining quality of sperm with sappan wood and purple cabbage extracts. The use of acid improves the quality of sperm staining with beet extract. There was no different between dyeing with beetroot extract in various solvent compositions and dyeing with Papanicolaou to color all sperm components (p> 0.05). The results showed that beetroot extract has a high potential to evaluate sperm morphology.
... HE and S staining, introduced by Edston and Grafti (1997) to substitute other combinations for staining specific tissues, provided impressive results. Natural saffron solution is used to identify collagen fibers, as it gives reproducible and reliable results (Ceccopieri et al., 2021). Collagen is one of the tissue components that adds strength to the organs and it protects structures, gives strength and elasticity, and helps the tissues to bear physical stress and tissue damages via its strong stiffened network. ...
The extracellular matrix (ECM) proteome represents an important component of the tissue microenvironment that controls chemical flux and induces cell signaling through encoded structure. The analysis of the ECM represents an analytical challenge through high levels of post‐translational modifications, protease‐resistant structures, and crosslinked, insoluble proteins. This review provides a comprehensive overview of the analytical challenges involved in addressing the complexities of spatially profiling the extracellular matrix proteome. A synopsis of the process of synthesizing the ECM structure, detailing inherent chemical complexity, is included to present the scope of the analytical challenge. Current chromatographic and spatial techniques addressing these challenges are detailed. Capabilities for multimodal multiplexing with cellular populations are discussed with a perspective on developing a holistic view of disease processes that includes both the cellular and extracellular microenvironment.
