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β-lactoglobulin (β-Lg) is a protein found in milk that can cause severe allergic reactions, including rash, vomiting, and diarrhea. Thus, it is crucial to develop a sensitive β-Lg detection method to protect people who are susceptible to allergies. Here, we introduce a novel and highly sensitive fluorescent aptamer biosensor for detecting β-Lg. Fir...
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... relative standard deviations for 1 ng mL −1 and 10 ng mL −1 of β-Lg were 2.44% and 4.43%, respectively, indicating good reproducibility of the assay. Currently, there are few studies detailing the use of DNA aptamers for β-Lg-detection-exploiting enzymes, and a comparison with other β-Lg assays is provided in Table 1. The approach demonstrated similar or better analytical performance compared with other β-Lg assays. ...Context 2
... relative standard viations for 1 ng mL −1 and 10 ng mL −1 of β-Lg were 2.44% and 4.43%, respectively, ind ing good reproducibility of the assay. Currently, there are few studies detailing the us DNA aptamers for β-Lg-detection-exploiting enzymes, and a comparison with other β assays is provided in Table 1. The approach demonstrated similar or better analytical formance compared with other β-Lg assays. ...Context 3
... relative standard viations for 1 ng mL −1 and 10 ng mL −1 of β-Lg were 2.44% and 4.43%, respectively, indi ing good reproducibility of the assay. Currently, there are few studies detailing the us DNA aptamers for β-Lg-detection-exploiting enzymes, and a comparison with other β assays is provided in Table 1. The approach demonstrated similar or better analytical p formance compared with other β-Lg assays. ...Citations
... β-lactoglobulin (β-LG) is a major milk allergen that can cause a range of allergic reactions, including skin irritation, respiratory distress, gastrointestinal issues, and even systemic allergic reactions [81]. To detect β-LG, several biosensors have been developed that employ innovative techniques to detect the allergen in real food samples [82]. ...
The excessive use of pesticides and drugs, coupled with environmental pollution, has resulted in the persistence of contaminants on food. These pollutants tend to accumulate in humans through the food chain, posing a significant threat to human health. Therefore, it is crucial to develop rapid, low-cost, portable, and on-site biosensors for detecting food contaminants. Among various biosensors, polymer-based biosensors have emerged as promising probes for detection of food contaminants in recent years, due to their various functions such as target binding, enrichment, and simple signal reading. This paper aims to discuss the characteristics of five types of food pollutants—heavy metals, pesticide residues, pathogenic bacteria, allergens, and antibiotics—and their adverse effects on human health. Additionally, this paper focuses on the principle of polymer-based biosensors and their latest applications in detecting these five types of food contaminants in actual food samples. Furthermore, this review briefly examines the future prospects and challenges of biosensors for food safety detection. The insights provided in this review will facilitate the development of biosensors for food safety detection.
Beta-lactoglobulin (β-Lg), a prominent milk protein, is a major contributor to milk allergies. The quantitative assessment of β-Lg is a valuable method for assessing the allergenic potential of dairy products. In this study, a specific aptamer, β-Lg-01, with an affinity constant (KD) of 28.6 nM for β-Lg was screened through seven rounds of magnetic bead SELEX (MB-SELEX). A novel bio-layer interferometry (BLI)-based aptasensor was developed, which had a limit of detection (LOD) of 0.3 ng mL-1, a linear range of 1.5 ng mL-1-15 μg mL-1, and a recovery rate of 102-116% among the milk samples. This aptasensor provides a potential tool for the detection and risk assessment of β-Lg within 10 min.
