-Comparison of DAMD and ISSR markers and their results and analyses computed for the Gladiolus cultivars

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... genetic distance values among all the 62 Gladiolus cultivars ranged from (0.10 to 0.48) with an average of 0.37. The mean values of expected heterozygosity (He) and Shannon's information index (I) was 0.234 ± 0.013 and 0.349 ± 0.019, respectively (Table 4). ...

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... primers 857 and 866 produced 100% polymorphism. The PIC values were calculated for each primer, and primer (Table 4). ...

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... cumulative genetic distance ranged from 0.14 to 0.46 with an average value of 0.36 among all the accessions of Gladiolus. The maximum inter-cultivar average genetic distance (0.46) was between Tiger Flame and Snow Flower cultivars, while the corresponding least genetic distance (0.14) was between Friendship Pink and Friendship White cultivars, respectively (Table 4). The cumulative analysis carried out for the data generated with DAMD and ISSR methods showed 83.32% polymorphism across all the Gladiolus cultivars. ...

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... higher values of PIC, DI, MI are in agreement with the good discriminatory potential of an efficient marker. However, these values are slightly higher in ISSR in comparison to DAMD marker but did not show any significant difference (Table 4). Mantel's test was performed for three data sets (DAMD, ISSR and cumulative), which revealed a significant correlation between ISSR v/s Cumulative, and showed the maximum (0.90) correlation coefficient (r), followed by DAMD v/s Cumulative (0.75), and DAMD v/s ISSR (0.39), respectively. ...