Table 3 - uploaded by Nishant Kumar
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Comparative chart of the allele distribution (genotype) of different loci of the DNA tested.
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Identifying the obscured exhibits is both a challenging and a commendable task in forensic science. Here we report on solving the case of a doctor's murder (victim) carried out for demand of gross ransom by five perpetrators. The crime scene happened in the Gumla District which lies in the Red Corridor of Jharkhand State (Eastern India). The incide...
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Citations
... The epigenetic markers present in the DNA of human cells or tissues can be used to distinguish the different tissues of same the human being, discern monozygotic twins, identify human races, predict human behaviour as well as to accurately determine the age of a DNA donor (Bekaert et al., 2015) using whole-genome bisulfite sequencing using Illumina Infinium BeadChip array (McCartney et al., 2016;Moran et al., 2016;, or methylated DNA immunoprecipitation sequencing (Borgel et al., 2012;Cheung et al., 2011;Mohn et al., 2009;Pelizzola & Molinaro, 2011;Sorensen & Collas, 2009;Taiwo et al., 2012;Zhao et al., 2014) as discussed above. Interestingly, when micro-volume blood stain, saliva laden cigarette stubs or few fallen hair strands are recovered from the crime scene, extremely low amounts of DNA (≤ 100 pg) as in the forensic case reported from Jharkhand (Rana et al., 2016), can be treated with bisulfite and then subjected to genome-wide amplification (Bundo et al., 2012), followed by quantitative methylation detection using the above technologies. The above methods have been found to produce comparable methylation results, but differ in CpG coverage, resolution, quantitative accuracy, efficiency, and cost. ...
Discerning monozygotic twins, precisely predicting the age of human beings and most importantly interpreting human behavior remains a big challenge in forensic science. Modern advances in the field of epigenetics especially analysis of DNA methylation pattern can certainly help to identify and discriminate various single-source DNA samples such as bodily fluids collected from the crime scene. In this review, cytosine methylation analysis of DNA has been methodologically discussed with a broad range of applications in forensic science such as identifying single or mixed body fluid, discriminating monozygotic twins, age prediction, addiction/behavioral prediction as well as race/ethnicity identification. With the advances of next-generation sequencing techniques, blooming of DNA methylation datasets and together with standard epigenetic protocols, the prospect of investigating and solving these critical issues in forensic science is highly promising in the court of law.
Identifying the obscured exhibits is both a challenging and a commendable task in forensic science. Here we report on solving the case of a doctor's murder (victim) carried out for demand of gross ransom by five perpetrators. The crime scene happened in the Gumla District which lies in the Red Corridor of Jharkhand State (Eastern India). The incident took place at two spots (atrocities for demand at a house and final murder in a forest) leaving behind some challenging evidences such as few scalp hairs on the bed, cigarette stubs, chewed tobacco and few blood drops scattered in a forest soil. These rare evidences were collected by the forensic team of Jharkhand and proceeded for DNA extraction and forensic analysis. The DNA obtained from these evidences matched with the DNA profile of the three suspects out of five under police detention. This is rare and first case reported in Jharkhand where chewed tobacco and few fallen hairs on bed sheet have been used to solve a critical crime case. Gumla police and the state forensic team were honoured with first prize in whole Jharkhand for solving this case scientifically.
