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The antagonistic potentials of actinomycetes isolated from coastal area of Niger Delta against Citrus sinensis (sweet orange) and Lycopersicum esculentum (tomato) fungal pathogens were investigated. Marine sediment and water samples were collected from ten (10) different points from Onne Light Flow Terminal Seaport situated in Eleme Local Governmen...
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... result in Table 3 showed that out of 15 strains, 5 (33.33 %) strains possess yellowish green aerial mycelia and 6 (40.00 %) strains possess pinkish brown substrate mycelia. The result in Table 4 showed that out of 15 strains, 9 (60.00 %) strains possess grayish green aerial mycelia and 7 (46.67 %) strains possess brown substrate mycelia. The result in Table 5 showed that out of 15 strains, 14 (93.33 %) strains possess grayish green aerial mycelia and dark brown substrate mycelia with melanoid pigment individually synthesized by strains MAB13 (Streptomyces cyaneus) and MAB15 (Streptomyces lavendulae). ...Similar publications
Industrial applications of lipase enzymes make them one of the most important enzymes in the biomanufacturing sector. Microorganisms contribute a huge percent of lipases in use today and the search for microorganisms from various habitat for lipase production is continuous. In this study, spread plate method on nutrient agar was used to isolate bac...
In my research work, I primarily focused on the investigation of yogurt (made from home-made raw milk) spoilage by microbiological and dielectric measurements. During the experiment, I continuously monitored the changes in aerobic and anaerobic Lactobacillus cell counts of the product, as a possible spoilage process would cause the deteriorative mi...
Food crop security and security is a challenge in the agricultural sector to produce quality and healthy food products for consumption by the community. The use of synthetic fungicides to induce plant resistance negatively affects the abundance and diversity of biological agents.Cellulose enzymes and chitinase enzymes are capable lytic enzymes that...
Background: Melon (Cucumis melo L.) is one of the most important fruit crops grown in China. However, the yield and quality of melon have significantly declined under continuous cropping. Phenolic acids are believed to be associated with the continuous monocropping obstacle (CMO) and can influence plant microbe interactions. Coumaric acid (CA) is o...
Actinomycetes are widely distributed in the environment and used for the production of several important secondary metabolites like antibiotics, immunosuppressive agents, enzymes and antitumor agents. Therefore, the main objective of this study is to isolate and assess antibacterial potential of different actinomycetes obtained from different soil...
Citations
... The results revealed that marine water (7.12 logCFU/mL) was shown to had more fungal Uba et al.; AJBGMB, 5(1): 17-27, 2020; Article no.AJBGMB.57693 18 count than the fresh water (7.07 logCFU/mL) ecosystem. ...
... Five treatments and the controls designs designated as 6.25%, 12 ...
... The suspected Aspergillus terreus fungal isolates were selected, purified by sub-culturing into new SDA plates and identified based on colonial and microscopic features such as colonial growth outline, conidial structure, and pigmentation. The technique described by Uba et al. [12] was also adopted for the identification of the isolated fungi by comparison with those of known taxa from a fungal key of identification atlas. ...
Aims: The present study aimed at assessing the toxicity effects of phone battery wastes on aquatic and terrestrial bioindicators. Study Design: Five treatments and the controls designs designated as 6.25%, 12.5%, 25%, 50%, 100%, CTRL and 1 mg/kg, 2 mg/kg, 3 mg/kg, 4 mg/kg, 5 mg/kg, 6 mg/kg, CTRL were set up in triplicates and incubated for 24 h, 72 h and 20 days at 25 ± 2°C. Place and Duration of Study: Methodology: The growth inhibition and mortality were analyzed using Aspergillus terreus toxicity test, Allium cepa toxicity test and Limicolaria flammea toxicity test. Results: The results revealed that marine water (7.12 logCFU/mL) was shown to had more fungal Original Research Article Uba et al.; AJBGMB, 5(1): 17-27, 2020; Article no.AJBGMB.57693 18 count than the fresh water (7.07 logCFU/mL) ecosystem. On A. terreus, Itel in fresh water sample had the highest LC 50 values of 30.49% while Gionee in fresh water sample had the lowest LC 50 values of 21.74% after 24 h, respectively. The Itel battery sample had higher EC 50 value (86.08%) than Gionee battery sample with EC 50 value of 65.46% after 72 h on A. cepa. On L. flammea, Itel phone battery sample had lower (5.11%) LC 50 value than Gionee phone battery sample with higher (6.20%) LC 50 value at 6 mg/kg concentration after 20 days' exposure, respectively. Conclusion: The results indicate that indiscriminate release of E-wastes into the aquatic and terrestrial environments should be discouraged.
... Methodology: The microbial growth inhibition was analysed using standard method of spread plate technique while growth indices and percentage seedling emergence were adopted for the seed growth inhibition. Uba et al.; AJOB, 9(2): 33-46, 2020; Article no.AJOB.57691 34 Results: The result revealed that fungal population was the most sensitive followed by bacterial population and then actinomycetes population in terms of toxic responses to the spent laptop battery samples soil contamination. ...
... After serially diluting of the samples up to 10 -3 , 0.1 mL aliquot was aseptically pipetted and dispensed on the surfaces of sterile Nutrient Agar (NA) (ketoconazole 0.025 %) plates, Potato Dextrose Agar (PDA) (chloramphenicol 0.005%) plates and Modified Glycerol Starch Casein Agar (MGSCA) (cycloheximide 50 µg/ mL); nystatin 25 µg/ mL) plates, respectively. The inoculants were evenly spread using a sterile glass rod and plates were incubated for 72 h at 25 ± 2°C for fungi; 24 h at 37 ± 2°C for bacteria and 96 h at 25 ± 2°C for actinomycetes [13,15]. The experiments were carried out in duplicates. ...
Aims: The study was undertaken to assess the toxicity potentials of spent laptop battery wastes on essential soil microbes and plant bio-indicators. Study Design: Five treatments and the controls designs were set up in triplicates and incubated at 25 ± 2°C for 21 days. The five treatments and controls set ups were designated as 6.25%, 12.5%, 25%, 50%, 100% and CTRL. Place and Duration of Study: Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University (COOU), Uli Anambra State, Nigeria during May, 2019 - July, 2019. Methodology: The microbial growth inhibition was analysed using standard method of spread plate technique while growth indices and percentage seedling emergence were adopted for the seed growth inhibition. Results: The result revealed that fungal population was the most sensitive followed by bacterial population and then actinomycetes population in terms of toxic responses to the spent laptop battery samples soil contamination. The order of toxicity of the spent battery samples on the growth indices and percentage seedling emergence of P. vulgaris and S. bicolor were: Product B-bean (-18.89%) > Product A-sorghum (-32.22%) > Product A-bean (- 38.63%) > Product B- sorghum (- 45.77%) revealing that both P. vulgaris and S. bicolor are very good bio-monitoring models for spent product A and B battery pollution assessment. Conclusion: Thus, strict and stringent measures on release of these electronic wastes in the environment are recommended.
Microcloning is an effective method of plant reproduction that is actively developing in Ukraine for the mass propagation of valuable food crops such as blackberries. However, the problem of losing a large number of microclones often arises during the ex vitro acclimatization stage. Inoculation of the rhizosphere with potentially useful microorganisms could have a positive impact on the survival rate and biometric characteristics of acclimatized seedlings. The aim of this study was to establish the impact of 23 actinobacteria isolates on blackberry microclones (Rubus fruticosus L.) during acclimatization to ex vitro conditions, and to determine the protective potential and plant growth-promoting effects of these bacteria. Methods. The antagonistic properties of the experimental microorganisms were determined using the agar block method. Bacteria were inoculated into the rhizosphere of blackberry microclones before planting in the soil. Results. The antagonistic activity of actinobacteria against phytopathogenic fungi P. expansum, P. variotii, A. niger, C. cladosporioides, F. oxysporum, A. alternata, R. cerealis, and A. tenuissima was established. The positive effect of bacteria on micropropagated blackberry plants during acclimatization to ex vitro conditions resulted in an increase in the survival rate of microclones in the soil by 34.8%, average height of experimental plants by 2.0 cm, node number by 3.4 nodes, and leaf area by 0.4 cm2. Conclusion. It was established that isolates of mycelial actinobacteria Lim4, Myt7ch, Conc32, Conc4 were promising inoculants for ex vitro acclimatization of micropropagated plants and could be recommended for the subsequent research in order to establish the interaction mechanisms between these microorganisms and plants.
Aim: To study the growth rate and antifungal activities of acetone extracts of Ocimum gratissimum (scent leaf) and Allium sativum (garlic) on cassava and banana peels formulated media. Study Design: Nine treatments and control designs were set up in triplicates and incubated at 25 for 72 h. The nine treatments and control set ups designated as Cassava Glucose Agar, CGA), Banana Glucose Agar, (BGA) and Control (Potato Dextrose Agar, PDA) were used to screen for the growth rate and antifungal activities of plant extracts. Place and Duration of Study: Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli Nigeria between June, 2019 and August, 2019. Methodology: The research study was carried on two formulated media obtained from cassava and banana peels and PDA) using proximate analysis, pour plate technique, agar plug diffusion technique and agar well diffusion technique. Results: The results revealed that the cassava and banana peels contained nutrients that can meet the nutritional conditions for fungi cultivation. The PDA statistically (P < 0.05) had higher mean radial growth (32.33 mm) and growth rate (0.449 h-1) than BGA (14.33 mm; 0.199 h-1) followed by CGA (14.16 mm; 0.197 h-1) in most of the test fungal isolates. The scent leaf acetone extract and nystatin antifungal had higher zones of inhibition (25.00 mm) than garlic acetone extract (24.00 mm) on the tested fungal isolates. There was no statistical significance at P < 0.05 on the inhibition zones of the extracts and Nystatin on the formulated media and PDA showing that the extracts possess comparable antifungal activities to the Nystatin. Conclusion: Thus, our formulated media were comparable to PDA with regards to antifungal activity of the acetone extracts and nystatin and could be utilized as an alternative and cheap ideal reference media for mycological assays.
