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Colistin resistant Kp209_CSTR has been sensitized to MAC-mediated killing due to enhanced PhoQ activation. (a) RNA was isolated from log phase Kp209 and Kp209_CSTR, and their transcriptomes were analysed. The volcano plot depicts differential expressed genes in Kp209_CSTR compared to Kp209. In Kp209_CSTR 124 genes were upregulated and 60 downregulated. (b,c) Kp209_CSTR transposon (Tn) library was exposed to 16% NHS, followed by re-exposed to 32% NHS, and surviving colonies were selected. Two unique Tn mutants had a transposon insertion in phoQ (Tn::phoQ). (b) Inner membrane permeabilization of Kp209, Kp209_CSTR, and Kp209_CSTR Tn::phoQ mutants in the presence of 10% NHS. (c) Inner membrane permeabilization of Kp209, Kp209_CSTR, and Kp209_CSTR Tn::phoQ mutants in the presence of 1 µg/ml colistin. (b,c) Bacteria were incubated at 37 °C in the presence of 1 µM SYTOX green nucleic acid stain, and inner membrane permeabilization (SYTOX fluorescence intensity) was detected after 60 min in a microplate reader. Data represent mean ± standard deviation of three independent experiments.
Source publication
Due to multi-drug resistance, physicians increasingly use the last-resort antibiotic colistin to treat infections with the Gram-negative bacterium Klebsiella pneumoniae. Unfortunately, K. pneumoniae can also develop colistin resistance. Interestingly, colistin resistance has dual effects on bacterial clearance by the immune system. While it increas...
