Clinical, histopatho­ logical and immunofluore­ scence staining features. (ac) Clinical and histopathological findings. (a) Serpiginous, raised tracts with vesicles on the trunk. (b, c) Histopathological findings (haematoxylin and eosin staining) of the lesions. Cross sections of Spiruroid type X larvae in the mid-dermis (b) surrounded by degranulated eosinophils, neutrophils and macrophages (c). (d-l) Immunofluorescence staining. (d) 2D7 + basophils (red) around the larvae. (e, f) Expression of interleukin (IL)-4 (green) (e) and IL-13 (green) (f) by 2D7 + basophils (red). (g-l) Extracellular DNA traps (ETs) identified with citrullinated histone H3 (CitH3, green). Major basic protein (MBP) + eosinophils (red) (g-i) and myeloperoxidase (MPO) + neutrophils (j-l) forming ETs. The blue areas indicate nuclei. *Larvae. Bars: 25 µm (i, l), 50 µm (c, e, f, h, k,), 100 µm (d, g, j) and 200 µm (b), respectively.

Contexts in source publication

Context 1

... 49-year-old, otherwise healthy, Japanese man presented with a 5-day-history of itchy, red moving eruptions 1 week after eating raw firefly squid. Physical examination revealed serpiginous, raised tracts with vesicles on his trunk (Fig. 1a). Laboratory tests showed peripheral blood eosinophilia (1,150/µl) with elevated serum levels of IgE (1,300 IU/ml; normal, <170 IU/ml) and thymus ...

Context 2

... of Spiruroid type X larvae in the mid-dermis, with massive infiltrates of degranulated eosinophils, neutrophils and macrophages surrounding the larvae (Fig. 1b, c). Based on a diagnosis of Spiruroid larva migrans due to Crassicauda giliakiana larvae, the anthelmintic drug ivermectin was administered, leading to gradual resolution of ...

Context 3

... staining showed massive dermal infiltration of 2D7 + basophils, major basic protein (MBP) + eosinophils and myeloperoxidase (MPO) + neutrophils, especially around the larvae (Fig. 1d, g, j). The infiltrating basophils expressed type 2 cytokines IL-4 and IL-13 (Fig. 1e, f). In addition, since eosinophils and neutrophils are known to be protective against parasites through forming extracellular DNA traps (ETs) (3), we investigated ETs from eosinophils (EETs) and neutrophils (NETs) using an ET-marker, citrullinated histone ...

Context 4

... staining showed massive dermal infiltration of 2D7 + basophils, major basic protein (MBP) + eosinophils and myeloperoxidase (MPO) + neutrophils, especially around the larvae (Fig. 1d, g, j). The infiltrating basophils expressed type 2 cytokines IL-4 and IL-13 (Fig. 1e, f). In addition, since eosinophils and neutrophils are known to be protective against parasites through forming extracellular DNA traps (ETs) (3), we investigated ETs from eosinophils (EETs) and neutrophils (NETs) using an ET-marker, citrullinated histone H3 (CitH3) (4, 5). CitH3 + web-like structures were found around the larvae, and ...

Context 5

... eosinophils and neutrophils are known to be protective against parasites through forming extracellular DNA traps (ETs) (3), we investigated ETs from eosinophils (EETs) and neutrophils (NETs) using an ET-marker, citrullinated histone H3 (CitH3) (4, 5). CitH3 + web-like structures were found around the larvae, and were seen to co-express MBP or MPO (Fig. 1h, i, k, l), indicating the formation of EETs and ...

Context 6

... study revealed massive lesional infiltration of basophils with eosinophils and neutrophils. Animal studies have shown that IgE-activated basophils infiltrate into the sites of parasitic infections and secrete a significant amount of IL-4/13, which leads to parasite expulsion partially through the activation of innate lymphoid cell type 2, recruitment of eosinophils, and induction of M2 macrophages secreting arginase-1 (1, 6). In humans, basophils might also have protective roles against parasites through these mechanisms. ...