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Classification of spermatogenic cycle stages in Bos indicus (Nellore) bulls. (A), classification A, presence of spermatogonias, primary spermatocytes and some elongated spermatids arranged in bundles. (B), segment classified as B, presence of the second meiotic division and secondary spermatocytes. (C) classification C, are found two generations of rounded and elongated spermatids and presence of sperm residual bodies.
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The aim of this study was to evaluate morphological, morphometric and functional aspects of spermatogenesis based on reproductive conditions of Nellore bulls. The study used 25 bulls Bos indicus (Nellore), which were classified as satisfactory (n=10) and unsatisfactory (n=15) for reproduction. After orchiectomy, fragments of the right testis of eac...
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... classified as A, in the initial stages, were represented by cells with proliferative or mitotic divisions of the seminiferous epithelium. The classifications B and C represent later stages of the epithelium, grouping tubules with meiotic divisions until the differentiation of pre-spermiation spermatozoa (Figure 2). A similar classification system was proposed by Horn et al. (2003) for crossbreed bulls (Bos indicus × Bos taurus) on the basis of the stages of the spermatic cycle described by Amann (1962); the results were similar. ...
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... Begitu juga kajian histomorfometri testis pada berbagai spesies lainnya sudah banyak dilaporkan, seperti histomorfometri testis sapi fulani putih (Togun 2009), sapi shorthorn (Swierstra 1966), sapi nelore (Silva et al. 2015), kambing hitam bengal (Gofur 2015), kambing kacang dan domba lokal (Noviana et al. 2000), unta (Ibrahim et al. 2012), babi (Straaten dan Wensing 1977), dan tikus putih wistar (Miraglia dan Hisakazu 1993). Menurut Togun (2009) ketebalan testis pada bagian tubulus seminiferous sapi fulani adalah 179,94 ± 4,64 µm,Swierstra (1966) menyebutkan ketebalan testis sapi shorthorn adalah 160,5 ± 18,9 µm, penelitian dariSilva et al. (2015) menyebutkan testis pada sapi nelore memiliki ukuran 70,0 ± 0,54 µm pada ketebalan epitel, 229,8 ± 1,22 µm pada diameter tubulus dan 3,8 ± 0,04 µm pada tunika propria.Gofur (2015) menyebutkan pada kambing hitam bengal memiliki kelenjar vasikular yang lebih panjang di banding dengan kelenjar bulbouretralisGambar 5 Gambaran histologi uretra pada penis kuda gayo. A. Lekukan pada uretra. B. Insert dari A. L = lumen, ES = Epitel Silindris Berlapis, LP = Lamina Propria (Pewarnaan HE, Perbesaran 40x (A) dan 400x (B))Tabel 1 Ketebalan lapisan tunika albugenia dan uretra pada penis kuda gayo ...
Penis kuda adalah alat kopulasi utama pada kuda. Penelitian ini bertujuan untuk mengetahui gambaran histologi danhistomorfometri penis pada kuda gayo. Sampel penelitian menggunakan penis dari 3 ekor kuda gayo jantan berumur 5-10 tahun yang dipotong di Rumah Potong Hewan Dolok Sanggul, Humbang Hasundutan, Sumatera Utara. Prosesmikroteknik dilakukan terhadap sampel menggunakan pewarnaan hematoksilin-eosin (HE). Pengamatan strukturhistologi menggunakan mikroskop dan dilakukan pengukuran ketebalan lapisan epitel uretra, ketebalan tunikaalbugenia di corpora cavernosa dan corpus spongiosum radix, corpus, dan glans penis menggunakan program aplikasitoupview. Hasilnya dibahas secara deskriptif. Hasil penelitian menunjukkan gambaran histologi penis kuda gayo terdiriatas dua jaringan erektil yaitu corpora cavernosa dan corpus spongiosum serta satu uretra. Sinusoid cavernosal daricorpus cavernosum mengandung banyak trabekula yang terdiri atas jaringan ikat fibroelastis, serat otot polos danfibroblas. Corpus spongiosum memiliki trabekula yang lebih tipis dengan ruang kavernosa yang lebih besar. Corpuscavernosum dan corpus spongiosum ditutup oleh tunika albuginea. Uretra terdiri atas berbagai epitel, seperti epitelberlapis transisional, epitel kolumnar berlapis dan epitel skuamosa. Pengukuran histomorfometri menunjukkan bahwaketebalan tunika albugenia radix dan corpus penis kuda gayo berturut-turut adalah 2.181,10 ± 48,50 µm, dan 2.366,51 ±131,48 µm., sedangkan ketebalan lapisan epitel uretra adalah 50,02 ± 6,95 µm. Kesimpulannya adalah penis kuda gayoterdiri atas radix, corpus dan glans penis. Radix dan corpus penis kuda gayo terdiri atas jaringan ikat, otot polos dansinusoid cavernosal; glans penis terdapat sinus uretra.
... Each testis was divided into longitudinal halves, so the rete testis could be identified in the longitudinal axis of the testis. Next, en bloc samples of approximately 1 cm 3 in size were taken from three different locations (proximal, middle, distal), as described by Brito et al. [17] and Da Silva [18]. The samples were placed into Bouin's solution for 48 hours. ...
the aim of this study was to test whether a single testicular needle biopsy could provide histological results comparable to en bloc resection histology and whether one biopsy was sufficient to reflect the histology of an entire pair of testicles. Two methods of sample collection were tested on 32 bull calves aged five to eight months to compare histological parameters of needle biopsy with those of en bloc resection samples. One testicular needle biopsy of the right and three en bloc samples of both testicles were collected and compared for the number of tubular cross sections, tubules with elongated spermatids (ES), outer/inner diameter of tubules, thickness of tubular wall, and number of Sertoli cells (SC). Additionally, animal data were considered. No significant differences were found between the left and right testis or among the individual locations of en bloc samples. However, histologically significant differences (Bonferroni-adjusted significance level: p < 0.05/6 = 0.0083) were found between the needle biopsy and en bloc resection regarding the tubular cross sections per visual field (p < 0.05), the outer (p = 0.01) and inner diameter and the thickness of the tubular wall (both p < 0.01). In the SOX9 immunohistochemical staining, no significant differences (p > 0.05) could be observed for SC numbers between needle biopsy and en bloc samples. In conclusion, results of testicular needle biopsy do not have the same validity as the en bloc resection histology. Furthermore, one biopsy is insufficient to reflect the histology of the entire testicular pair.
