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Chromosome abnormalities in the various phases of mitotic cell division of naphthalene solution treated onion root tips.
Source publication
The cytotoxic effect of naphthalene was assessed in the root meristem of Allium cepa L. - a model plant for
chromosomal study. The newly emerging roots were treated with 10%, 30%, 50%, 75% and 100% naphthalene solution (v/v). Each treatment was kept for 6, 12, 18, 24 and 48 hours respectively to study their cytotoxic effect. Naphthalene exerted dep...
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The continuous production and release into the environment of chemicals has revealed the need to determine their cytotoxicity and genotoxicity. Sulfonamide-aldehyde (SA) derivatives, whose biological activity properties vary in a wide spectrum, are frequently used in agriculture, medicine, pharmacy and many other fields. These compounds have an imp...
Citations
... For 1 hour, root tips were hydrolyzed in 5N HCl at room temperature. The root tip is stained with 2% acetoorcein, which is then mashed up in 45% acetic acid (Hore and Tanti, 2014;Sarma et al., 2017). Root tip portion is prepared by being cut out and squashed (Sarma and Tanti, 2015). ...
... Each concentration was given a mean value and standard deviation. For each treatment 1000 cells were evaluated on one slide (Sarma et al., 2017). The number of dividing cells per total number of cells assessed is how the mitotic index (MI) was calculated: ...
Farmers’ preference for cultivation of Mung bean (Vigna radiata) especially in the activity of sprout germination can exhibit zestful features while in bio-physiological and metabolical actions. Desiring for improved use of this habitually consumed crop for its ranges of medicinal values which possesses anti-bacterial, anti-oxidant, anti-hyperglycemic, anti-hypertensive, anti-inflammatory, anti-diabetic, metabolic accommodation of lipid, and antitumor effect, and many more scientists are working on it in order gather more evidences. The current study utilised concentrations of 50, 100, 500, and 1000 ppm in mung bean somatic cells to determine the toxicity of 2,4-D. Each concentration is treated for a length of 24, 48, 72, and 96 hours. Low concentration (50 ppm) pollutants were exposed for 24 hours in Mung bean, and the mitotic index decreased with the increased exposure times of 24, 48, 72, and 96 hours. Mitotic indexes dropped, whereas chromosomal abnormalities rose. The mitotic index had a propensity to decline as pollution concentrations rose concurrently. Some of the common anomalies seen across all treatments include C-metaphase, star shaped, binucleate, micronuclei, sticky anaphase erosion, chromosomal distributional error, chromosomal clumping, and failure of cell plate formation. Chromosome stickiness at 50 ppm, increased cell size at 100 ppm, chromosomal compression at 500 ppm, and chromosomal disintegration at 1000 ppm are relatively prevalent among all the treatments. For such crops, which have numerous uses, biomonitoring is necessary. This study can provide guidelines for determining the proper pesticide dose for extensive farming.
Vanilla borneensis Rolfe is a monopodial climbing orchid growing naturally in the state of Assam, India. It has been reported as a rare, endemic and threatened terrestrial orchid. In the present study, karyotypic analysis of the V. borneensis was carried out to characterize the chromosome content. Somatic chromosome number in V. borneensis was found with 2n = 28, out of which 13 were median, 11 metacentric and 4 sub-metacentric chromosomes. This report can be considered as the first cytological report of the V. borneensis which is endemic to Assam in India and can be of valuable information for further taxonomic identification as well as genetic characterization of the group.
