Chromatograms using different solvents as a mobile phase. (a) Petroleum ether : acetone (8 : 2) revealed with vanillin: spots a (Rf = 0.31), b (Rf = 0.28), and c (Rf = 0.65) indicate the presence of mono- or sesquiterpenes; (b) toluene : acetone (8 : 2) revealed with vanillin: samples 2, 6, 7, 8, and 10 have a common spot; (c) chromatograms of samples number 3 and 5 obtained with dichloromethane : ethanol in an 8 : 2 ratio, revealed with (C1) vanillin, (C2) long-wave UV light (365 nm), and (C3) short-wave UV light (254 nm).

Chromatograms using different solvents as a mobile phase. (a) Petroleum ether : acetone (8 : 2) revealed with vanillin: spots a (Rf = 0.31), b (Rf = 0.28), and c (Rf = 0.65) indicate the presence of mono- or sesquiterpenes; (b) toluene : acetone (8 : 2) revealed with vanillin: samples 2, 6, 7, 8, and 10 have a common spot; (c) chromatograms of samples number 3 and 5 obtained with dichloromethane : ethanol in an 8 : 2 ratio, revealed with (C1) vanillin, (C2) long-wave UV light (365 nm), and (C3) short-wave UV light (254 nm).

Source publication
Article
Full-text available
Aim: To evaluate the efficacy of different brands of cigarettes in the preparation of tobacco agar, for the differentiation of these related yeasts. Methodology. Tobacco agar was prepared using six brands and four varieties of cigarettes, and 125 clinical isolates previously identified by PCR and Maldi-Tof were used. To determine whether the resul...

Similar publications

Article
Full-text available
PurposeEmbryo genotyping in IVF clinics aims to identify aneuploid embryos, and current methodologies rely on costly, invasive and time-consuming approaches such as PGT-A screening. MALDI-ToF-based mass spectral analysis of embryo culture has been demonstrated to be a non-invasive, affordable and accurate technique that is able to capture secretome...