Table 2 - uploaded by Mohamed Bnouham
Content may be subject to copyright.
Chromatogram data (Retention time, area, percent and compound name) of Ptychotis verticillata essential oil.
Source publication
Abstract
AIM: Ptychotis verticillata (Apiaceae), an endemic medicinal plant of Morocco. This plant is traditionally used for treatment of many diseases. The aim of the present study was to identify the constituents of the essential oil of P. verticillata (EOPV) and to carry out an evaluation of their antimicrobial activity.
METHODS: Chemical compos...
Similar publications
Microbial infections are major public health problems in the developing countries. Infectious disease consider as the most cause of death for approximately one-half of all deaths in tropical countries. This study was carried out to investigate the in-vitro antibacterial activity of Acacia nilotica methanolic fruits extract against clinical isolates...
Micro algae Spirulina platensis, Haematococcus pluvialis and Botryococcus braunii are cultured commercially and their productions are established in different parts of the world. In the present investigation the antibacterial properties of different solvent extracts of these three micro algae were evaluated. The maximum phenolic contents (128, 131,...
In response to the propagation of bacteria resistant to many antibiotics, the discovery of new and more efficient antibacterial agents is primordial. The present study was designed to evaluate the antimicrobial activity of three extracts of medical plants, Cichorium intybus, Dorema aucheri and Prangos ferulacea against some food born pathogens. The...
In this study, antimicrobial activity of various extracts prepared from Hyla arborea skin secretion have been investigated for their antimicrobial activity. Antimicrobial activity was determined with Escherichia coli ATCC 11230, Staphylococcus aureus 6538-P, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27583, Proteus vulgaris ATCC 8427,...
Prosopis juliflora is the most widespread species of genus Prosopis and it is a good source of compounds that have been shown to be pharmacologically active. This plant has been used as a traditional treatment for several diseases such as microbial infections are major public health problems in the developed and developing countries. Infectious dis...
Citations
... These molecules have sparked significant interest in medical research owing to their bioactivity, including their ability to neutralize free radicals and protect cells against oxidative damage [31]. Traditionally referred to as "Nûnkha," this plant is utilized for various purposes such as flavouring [32], perfumery, and the treatment of digestive and respiratory disorders. Several studies have highlighted the abundance of terpene compounds in this plants essential oil, including carvacrol, thymol, D-limonene and gamma-terpinene [29,33,34]. ...
... A further investigation carried out by Bnouham et al. revealed that the crucial oil derived from P. verticillate, there were a total of 10 components discovered. The main constituents were thymol, D-limonene, gamma-terpinene, and m-cymene, which accounted for 95.86 % of the total [32]. These findings contribute to our understanding of the chemical composition of PVEO and its potential applications in various fields, including pharmacology and aromatherapy. ...
The aim of this study is to explore the pharmacological properties of the essential oil derived from Ptychotis verticillata Duby (PVEO), a medicinal plant native to Morocco, focusing on its an-tidiabetic, anti-tyrosinase, and anti-inflammatory effects. Additionally, the study aims to characterize the phytochemical composition of PVEO and evaluate its potential as a natural therapeutic alternative for various health conditions. To achieve this, phytochemical analysis was conducted using gas chromatography-mass spectrometry (GC-MS). Furthermore, in vitro assessments were conducted to investigate PVEO's antidiabetic activity by inhibiting α-amylase, xan-thine oxidase, and α-glucosidase. Tests were also undertaken to evaluate the anti-inflammatory effect of PVEO on RAW 264.7 cells stimulated by lipopolysaccharide (LPS), as well as its efficacy as an anti-tyrosinase agent and its lipoxygenase inhibition activity. The results of the phyto-chemical analysis revealed that PVEO is rich in terpene compounds, with percentages of 40.35 % γ-terpinene, 22.40 % carvacrol, and 19.77 % β-cymene. Moreover, in vitro evaluations Note: Low-resolution images were used to create this PDF. The original images will be used in the final composition. C O R R E C T E D P R O O F M. Taibi et al. Heliyon xxx (xxxx) e29459 demonstrated that PVEO exhibits significant inhibitory activity against α-amylase, xanthine oxi-dase, and α-glucosidase, indicating promising antidiabetic, and anti-gout potential. Furthermore , PVEO showed significant anti-tyrosinase activity, with an IC50 of 27.39 ± 0.44 μg/mL, and remarkable lipoxygenase inhibition (87.33 ± 2.6 %), suggesting its candidacy for dermato-protection. Additionally, PVEO displayed a dose-dependent capacity to attenuate the production of NO and PGE2, two inflammatory mediators implicated in various pathologies, without compromising cellular viability. The findings of this study provide a solid foundation for future research on natural therapies and the development of new drugs, highlighting the therapeutic potential of PVEO in the treatment of gout, diabetes, pigmentation disorders, and inflammation.
... Ptychotis verticillata, known as Nûnkha and by other several names, including Psychotis ammoides Koch., Petroselium ammoides Rchb. Fil., Ammoides pusilla (Brot.), and Breistr., Ammoides verticillata Briq., is a medicinal and aromatic plant widespread in North African countries and widely used in traditional medicine as an antispasmodic, antidiabetic, and antipyretic agent, as well as an antiseptic, and for its antifungal and antibacterial properties [15][16][17]. The medicinal plant P. verticillata has been known to have several therapeutic effects and medical benefits, yet, to our understanding, there are very few studies that have explored and reported on its antioxidant and antimicrobial properties in depth. ...
... The differences in the oil's chemical composition may be due to a variety of factors, including biotic and abiotic factors, the age of the plant, the period of its vegetative cycle, or even genetic factors [18,19]. It is worth noting that numerous studies have demonstrated that plants with similar chemical compositions to P. verticillata exhibit diverse biological activities, such as antioxidant, antibacterial, antifungal, and insecticidal properties [16,20]. ...
Ptychotis verticillata Duby, referred to as Nûnkha in the local language, is a medicinal plant that is native to Morocco. This particular plant is a member of the Apiaceae family and has a longstanding history in traditional medicine and has been utilized for therapeutic purposes by practitioners for generations. The goal of this research is to uncover the phytochemical makeup of the essential oil extracted from P. verticillata, which is indigenous to the Touissite region in Eastern Morocco. The extraction of the essential oil of P. verticillata (PVEO) was accomplished through the use of hydro-distillation via a Clevenger apparatus. The chemical profile of the essential oil was then determined through analysis utilizing gas chromatography–mass spectrometry (GC/MS). The study findings indicated that the essential oil of P. verticillata is composed primarily of Carvacrol (37.05%), D-Limonene (22.97%), γ-Terpinene (15.97%), m-Cymene (12.14%) and Thymol (8.49%). The in vitro antioxidant potential of PVEO was evaluated using two methods: the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical trapping assay and the ferric reducing antioxidant power (FRAP) method. The data demonstrated considerable radical scavenging and relative antioxidative power. Escherichia coli, Staphylococcus aureus, Listeria innocua, and Pseudomonas aeruginosa were the most susceptible bacterial strains tested, while Geotrichum candidum, Candida albicans, and Rhodotorula glutinis were the most resilient fungi strains. PVEO had broad-spectrum antifungal and antibacterial properties. To elucidate the antioxidative and antibacterial characteristics of the identified molecules, we applied the methodology of molecular docking, a computational approach that forecasts the binding of a small molecule to a protein. Additionally, we utilized the Prediction of Activity Spectra for Substances (PASS) algorithm; Absorption, Distribution, Metabolism, and Excretion (ADME); and Pro-Tox II (to predict the toxicity in silico) tests to demonstrate PVEO’s identified compounds’ drug-likeness, pharmacokinetic properties, the anticipated safety features after ingestion, and the potential pharmacological activity. Finally, our findings scientifically confirm the ethnomedicinal usage and usefulness of this plant, which may be a promising source for future pharmaceutical development.
... While the yield of essential oil of A. verticillata is 6% harvested in Tlemcen regions, and in other regions, the extraction yields of its essential oils vary from 2.1 to 5.4% [23]. Much lower rates were reported at 2.51% by Bnouham in the region of Oujda [24], 2% in Ihfir (Morocco) [25,26], 2.8% by Khajeh [27], 2.7% by Bendahou (2007) [27], and 3.5% by Chialva (1993) [28]. ...
Background:
Potatoes are extremely important compared to other vegetable crops. Several species of fungi cause severe damage to different components of the plant (leaves, stems, and tubers), leading to significant losses during cultivation and even after harvest. In the framework of the investigation for alternative methods against the proliferation of these fungi, the present work focuses on the study of the antifungal effect of essential oils of some plants that could be used to solve these problems without the use of harmful chemical substances.
Aim and purpose:
This study aims to discuss the chemical composition of essential oils of Artemisia herba alba and Ammoides verticillata and evaluate their in vitro and in vivo antifungal activities in order to prevent fungal diseases of potatoes and replace chemical pesticides that cause neurodegenerative diseases.
Materials and methods:
Essential oils extracted from the aerial parts of the plants Artemesia herba alba and Ammoides verticillata were analyzed by gas chromatography-mass spectrometry (GC/MS) and tested individually and in combination for their antifungal effects against Fusarium solani, Penicillium expansum, and Aspergillus flavus, by the radial growth technique.
Results:
The essential oil of A. herba alba was mainly composed of hydrocarbon monoterpenes (80.8%), while the oil of A. verticillata was mainly composed of oxygenated monoterpenes (54.4%). The study on the antifungal effect of essential oils in vitro showed that essential oil of A. Verticillata was more effective against P. expansum (64.40%) than A. flovus (41.10%) and F. solani (53.30%), and the oil of A.herba alba was more effective against A. flavus (54.40%) and (42%) F. Solani. While the combination of these two essential oils of A. verticillata and A. herba-alba gave excellent results, i.e., 100% against P. expansum and A. flavus and 94.40% against F. solani and provided an in vivo protection to the potato in the range of 80% to 90% against the three fungi.
Conclusion:
A. herba alba and A. verticillata individual and combined essential oils are very effective antifungal biocides that can be used as an alternative to chemical pesticides to prevent their harmful effects on health.
... Ptychotis verticillata known as Nûnkha and by other several names including Psychotis ammoides Koch., Petroselium ammoides Rchb. Fil., Ammoides pusilla (Brot.), and Breistr., Ammoides verticillata Briq., is a medicinal and aromatic plant widespread in North African countries and widely used in the traditional medicine as antispasmodic, antidiabetic and antipyretic as well as antiseptic for its antifungal and antibacterial properties [7][8][9]. The medicinal plant P. verticillata has been known to have several therapeutic effects and medical benefits, yet, to our understanding, there are very few studies that have explored and reported on its antioxidant and antimicrobial properties in depth. ...
... The variations in chemical composition may be due to several factors including biotic and abiotic factors, the age of the plant, the period of its vegetative cycle, or even genetic factors [30,31]. It is important to note that several studies have shown that plants with similar chemical compositions to P. verticillata possess various biological activities such as antioxidant, antibacterial, antifungal, and insecticidal properties [8,32]. This preprint research paper has not been peer reviewed. ...
... In Morocco, Nûnkha is an aromatic and medicinal plant mainly found in the eastern region [5]. PV is widely used in traditional medicine as an infusion to treat headache, fever, flu, diarrhea, diabetes, and hypertension [16]. The use of individual compounds or in combination rather than the essential oil is a relevant question to be investigated. ...
Acute myeloid leukemia (AML) is a cancer of the myeloid lineage of blood cells, and treatment for AML is lengthy and can be very expensive. Medicinal plants and their bioactive molecules are potential candidates for improving human health. In this work, we studied the effect of Ptychotis verticillata (PV) essential oil and its derivatives, carvacrol and thymol, in AML cell lines. We demonstrated that a combination of carvacrol and thymol induced tumor cell death with low toxicity on normal cells. Mechanistically, we highlighted that different molecular pathways, including apoptosis, oxidative, reticular stress, autophagy, and necrosis, are implicated in this potential synergistic effect. Using quantitative RT-PCR, Western blotting, and apoptosis inhibitors, we showed that cell death induced by the carvacrol and thymol combination is caspase-dependent in the HL60 cell line and caspase-independent in the other cell lines tested. Further investigations should focus on improving the manufacturing of these compounds and understanding their anti-tumoral mechanisms of action. These efforts will lead to an increase in the efficiency of the oncotherapy strategy regarding AML.
... and commonly named Nûnkha, 29,30 is an aromatic herbaceous species (10-35 cm tall), endemic to northwestern Algeria and used for its culinary and medicinal properties as a febrifuge, antispasmodic, antiseptic and antidiabetic, especially in decoctions and infusions. 31,32 Moreover, the infusions and ethanolic extracts have been reported to have a wide diversity of polyphenols (flavonoids, saponins, and tannins), while its essential oils are mainly characterized by the presence of thymol and carvacrol. 33 L. guyonianum (Plumbaginaceae family) is a medicinal halophyte species endemic to the north of Africa; the infusion of its leaves and galls are traditionally used as an anti-dysenteric against infectious diseases or parasites responsible for painful and bloody diarrhea. ...
Infusions of Thymus pallescens Noë, Saccocalyx satureioides Coss. et Dur., Ptychotis verticillata Briq. and Limoniastrum guyonianum Boiss. have been used as medicinal remedies for many diseases in the Algerian folk medicine. These species have also been well documented as rich sources of phytochemicals, such as phenolic compounds with wide diversified chemical structures, which results in far-ranging biological activities. Thus, the phenolic compounds profile of the aqueous extracts, obtained by infusing, of the mentioned species was obtained by HPLC-DAD-ESI/MS, and their antibacterial activity was evaluated against clinical isolates. Several phenolic acids were identified and quantified, particularly caffeic acid derivatives along with glycosylated flavonoids. T. pallescens and S. satureioides contain 13 phenolic compounds, where rosmarinic acid was the most abundant phenolic acid present, while L. guyonianum presented myricetin-3-O-glucoside and myricetin-O-rhamnoside as the main compounds among the eight detected molecules. P. verticillata presented a profile of ten phenolic compounds, where 5-O-caffeoylquinic acid was the most abundant phenolic acid, followed by the flavone luteolin-3-O-glucoside. Antibacterial activity of the infusions ranged between 2.5 and 20 mg/mL (MIC values), and L. guyonianum showed the highest activity against all the tested bacteria, being Staphylococcus aureus and Pseudomonas aeruginosa the most sensitive and resistant strains, respectively. Thus, the studied plant species are sources of natural antibacterial substances that can be used to fight against pathogenic microorganisms.
... However, these types of components were not detected in this oil and reflect the activity. Moreover, no activity was observed on the other strains including fungi, could be explained by the migration of the active constituents into the water during the hydrodistillation process 13 . ...
The essential oil isolated by hydrodistillation from fresh whole plant parts of Piper muricatum
Blume (Piperaceae) was analyzed by GC and GC-MS. Forty components were identified, representing 90.8 %
of the total oil. The major components were aromadendrene (16.2 %), β-caryophyllene (8.8 %), germacrene D
(7.9 %), γ-cadinene (7.9 %), elemol (5.4 %), γ-elemene (4.9 %) and bicyclogermacrene (4.4 %). The antimicrobial
evaluation revealed that the essential oil had moderate activity towards Gram positive bacteria, Bacillus cereus
and Streptococcus mutans each with MIC value of 250 μg/mL.
