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The high performance liquid chromatographic (HPLC) method was developed for the combined estimation of sodium alginate and pectin in raft forming pharmaceuticals on C 18 column ZORBAX ODS (1.5 cm × 4.6 mm, 5 μm) with UV detection at 378 nm. The assay condition comprised of phosphate buffer pH 7.4 and methanol 60:40% v/v at a flow rate of 1.25 mL/mi...
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Context 1
... environment, while in alkaline media, rapid dissolution occurs, which leads to instability and limits its applications. Sodium alginate swells in water but typically produces an acid pH gel due to its protonation and can be crosslinked by the application of physical and chemical methods [1]. The chemical structure of alginate, as mentioned in Fig. 1, has a linear copolymer of 1, 4-glycosidically linked b D-mannuronic acid and a-L-guluronic acid. Pectin, illustrated in Fig. 1, is an abundantly available natural polysaccharide generally present in the cell wall of plants, while its structure is composed of uronic acid residues which are linked together by a-1, 4-glycosidic bonds ...
Context 2
... alginate swells in water but typically produces an acid pH gel due to its protonation and can be crosslinked by the application of physical and chemical methods [1]. The chemical structure of alginate, as mentioned in Fig. 1, has a linear copolymer of 1, 4-glycosidically linked b D-mannuronic acid and a-L-guluronic acid. Pectin, illustrated in Fig. 1, is an abundantly available natural polysaccharide generally present in the cell wall of plants, while its structure is composed of uronic acid residues which are linked together by a-1, 4-glycosidic bonds [2]. The pectin is favored in formulations due to its resistance property against certain enzymes such as amylase and protease but ...
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The high performance liquid chromatographic (HPLC) method was developed for the combined estimation of sodium alginate and pectin in raft forming pharmaceuticals on C 18 column ZORBAX ODS (1.5 cm 3 4.6 mm, 5 mm) with UV detection at 378 nm. The assay condition comprised of phosphate buffer pH 7.4 and methanol 60:40% v/v at a flow rate of 1.25 mL/mi...
Citations
... UV detection was carried out at 200 nm. The system was equilibrate with the mobile phase for 45 min before injection [28]. ...
Seaweeds are free-living macroalgae that are simple to grow since they do not require freshwater or rich soil. The low lignin concentration and high alginate, mannitol, and laminarin content in seaweed biomass make it a promising candidate for bioconversion. This study investigates the feasibility of a co-culture approach for simultaneous alginate degradation and acetone-butanol-ethanol (ABE) production using alginolytic Enterobacter hormaechei subsp. xiangfangensis SW2 isolated from degraded seaweed and solventogenic Clostridium acetobutylicum DSM 792. Molecular techniques were used to characterize the isolated E. hormaechei subsp. xiangfangensis SW2. The co-culture fermentation, which lasted 120 h, produced 9.02 g/L and 8.5 g/L of butanol, utilizing sodium alginate and alginate extract from macroalgae as the substrate. Both bacterial species showed compatible growth during alginate fermentation, indicating a prospective technique for sustainable solvent synthesis using seaweed alginate.
... UV detection was carried out at 200 nm. The system was equilibrate with the mobile phase for 45 min before injection [28]. ...
