| Characterization of the extracorporeal photopheresis product. (A) Impact of ECP on the viability of Lew DA . Representatives dot plots from Lew DA cells, ECP cells, ECP cells cultured with full medium for 3 days and ECP cells cultured with phytohemagglutinin-L (PHA-L) for 3 days. In order to differentiate alive cells, early and late apoptotic cells, samples were stained with a viable die and Annexin V. (B) Impact of ECP on the proliferative capacity of Lew DA splenonocytes and ECP product using CFSE staining. Left plot, Lew DA splenocytes cultured for 3 days with or without PHA-L (red and blue lines, respectively). Right plot, ECP cells cultured for 3 days with or without PHA-L (red and blue lines, respectively). Gray shaded areas, cells stained with CFSE at Day 0.

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... ECP cell product's viability was assessed immediately after the photopheresis and after 3 days in culture with or without PHA-L as a mitogenic stimulus. ECP cell product seems to be alive (79.4 ± 1.4%) when the analysis was performed immediately after the photopheresis procedure compared to allogeneic Lew DA cells ( Figure 5A). However, when the ECP cell product was cultured for 3 days with or without PHA-L, about 95.2 ± 3.6% and 94.0 ± 0.2% of cells had to be considered apoptotic, respectively; moreover, just about 0.15 ± 0.06% and 0.14 ± 0.10% of cells remained alive, respectively ( Figure 5A). ...

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... cell product seems to be alive (79.4 ± 1.4%) when the analysis was performed immediately after the photopheresis procedure compared to allogeneic Lew DA cells ( Figure 5A). However, when the ECP cell product was cultured for 3 days with or without PHA-L, about 95.2 ± 3.6% and 94.0 ± 0.2% of cells had to be considered apoptotic, respectively; moreover, just about 0.15 ± 0.06% and 0.14 ± 0.10% of cells remained alive, respectively ( Figure 5A). ...

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... order to evaluate the ECP cell product's proliferative capacity, a mitogenic stimulus was added in the cell culture media. The photopheresis procedure completely avoided the proliferation observed in Lew DA cells ( Figure 5B). Figure S2), whereas a low dose of ECP did not confer any improvement in renal function compared to the TAC group. ...

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... ECP cell product's viability was assessed immediately after the photopheresis and after 3 days in culture with or without PHA-L as a mitogenic stimulus. ECP cell product seems to be alive (79.4 ± 1.4%) when the analysis was performed immediately after the photopheresis procedure compared to allogeneic Lew DA cells ( Figure 5A). However, when the ECP cell product was cultured for 3 days with or without PHA-L, about 95.2 ± 3.6% and 94.0 ± 0.2% of cells had to be considered apoptotic, respectively; moreover, just about 0.15 ± 0.06% and 0.14 ± 0.10% of cells remained alive, respectively ( Figure 5A). ...

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... cell product seems to be alive (79.4 ± 1.4%) when the analysis was performed immediately after the photopheresis procedure compared to allogeneic Lew DA cells ( Figure 5A). However, when the ECP cell product was cultured for 3 days with or without PHA-L, about 95.2 ± 3.6% and 94.0 ± 0.2% of cells had to be considered apoptotic, respectively; moreover, just about 0.15 ± 0.06% and 0.14 ± 0.10% of cells remained alive, respectively ( Figure 5A). ...

Context 6

... order to evaluate the ECP cell product's proliferative capacity, a mitogenic stimulus was added in the cell culture media. The photopheresis procedure completely avoided the proliferation observed in Lew DA cells ( Figure 5B). Figure S2), whereas a low dose of ECP did not confer any improvement in renal function compared to the TAC group. ...