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Changes in skin hydration in individuals receiving Low-molecular-weight Collagen peptide (LMWCP) preparation or placebo. Skin hydration was measured with a Corneometer CM825, and changes from baseline values are shown, with arbitrary units (AU). Data are expressed as the mean ± SEM. *** indicates a significant difference from the baseline (p < 0.001; t-test). †† and ††† indicate significant differences between the test group and the placebo group (p < 0.01 and p < 0.001, respectively; t-test).
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Collagen-peptide supplementation could be an effective remedy to improve hydration, elasticity, and wrinkling in human skin. The aim of this study was to conduct a double-blind, randomized, placebo-controlled trial to clinically evaluate the effect on human skin hydration, wrinkling, and elasticity of Low-molecular-weight Collagen peptide (LMWCP) w...
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Citations
... Numerous studies have reported that the supplementation of collagen or its tripeptides improves skin wrinkles, elasticity, and hydration [13][14][15]. Collagen tripeptides can be a potent anti-aging component, enhancing the synthesis of type 1 collagen and suppressing matrix metalloproteinase (MMP) protein expression [5,16]. A recent study reported hydrolyzed marine collagen ameliorates skin health in an aging population by improving skin wrinkles, elasticity, hydration, radiance, and firmness [17]. ...
... The average molecular weight of the entire Tripep20 collagen was 500 Da, with a total content of 20% tripeptides containing 3.2% Gly-Pro-Hyp. Other in vivo studies confirmed the stability and bioavailability of Gly-Pro-Hyp [11,14]. A 50 ml bottle contained 1,000 mg of Tripep20 and vehicle materials ( Table 1). ...
... Based on the guidelines for estimating the maximum safe dose in initial clinical trials of healthy subjects, 1,000 mg of Tripep20 appeared suitable for humans with an average body weight of 60 kg [18]. In addition, several comparable studies using doses from 1.0 to 3.0 g/ day have been reported [14,[19][20][21]. ...
BACKGROUND/OBJECTIVES: Collagen is commonly used in diverse forms as a functional component in skincare products. On the other hand, the effects of collagen on human skin are controversial. Dietary collagen hydrolysates from freshwater Pangasius hypophthalmus fish skin ameliorated photo-aged skin of hairless mice. This study conducted a randomized, double-blind, placebo-controlled clinical trial to determine if liquid fish collagen (Collagen-Tripep20™, Tripep20) as a drink strengthens skin health and quality. SUBJECTS/METHODS: In this clinical trial, 85 subjects aged 35-60 yrs were diagnosed with photo-aged skin. Eighty-five subjects were randomized to receive either Tripep20 (n = 44) or placebo (n = 41). Seventy-eight subjects fully participating for a 12-week period consumed 1,000 mg of Tripep20 (n = 41) or placebo (n = 37) in a 50-mL bottle as a daily drink. The intend-to-treat and per-protocol populations were 85 and 78, respectively. Skin hydration, wrinkles, and elasticity were assessed at 0 (baseline), 6, and 12 weeks during the study period. RESULTS: Skin hydration in the Tripep20 group was significantly higher from 6 weeks (P < 0.001) than the baseline. After 12 weeks, the crow's-feet visual score and skin roughness (R a , R q , and R max) were significantly improved in the Tripep20 group than in the placebo group (P < 0.05). Consuming liquid collagen Tripep20 greatly enhanced skin elasticity (gross R2, net R5, and biological elasticity R7) in 6 weeks compared to the placebo group. The Tripep20 group showed a significant increase in skin elasticity from the baseline after 6 and 12 weeks (P < 0.001). Neither abnormal symptoms nor adverse events were encountered during the study period in subjects ingesting Tripep20 or placebo. The changes in parameters related to hematology and clinical chemistry were within the normal ranges. CONCLUSION: Oral consumption of liquid collagen Tripep20 was safe and well-tolerated. The results of this study show that freshwater fish-derived liquid collagen Tripep20 can be used as a healthy functional food ingredient to improve skin moisturizing, anti-wrinkling, and elasticity in an aging population.
... Our study found that the content of HYP and HA in the skin of aging mice increased, the expressions of filaggrin, involucrin, and loricrin mRNA were up-regulated, and the hydration ability of the skin of mice was improved. The results were consistent with those of Kim et al. [41]. Improving the hydration ability of the skin can keep the skin moist, reduce the generation of wrinkles, and improve the roughness of the skin, thus playing an anti-aging role. ...
Skin problems caused by aging have attracted much attention, and marine collagen peptides have been proved to improve these problems, while mammalian collagen peptides are rarely reported. In this study, fermented deer bone collagen peptide (FCP) and non-fermented deer bone collagen peptide (NCP) were extracted from fermented and non-fermented deer bone, respectively, and their peptide sequences and differential proteins were analyzed using LC-MS/MS technology. After they were applied to aging mice induced with D-gal, the skin hydration ability, antioxidant ability, collagen synthesis, and degradation ability of the mice were studied. The results show that FCP and NCP are mainly peptides that constitute type Ⅰ collagen, and their peptide segments are different. In vivo experiments show that FCP and NCP can improve the richness of collagen fibers in the skin of aging mice; improve the hydration ability of skin; promote the activity of antioxidant-related enzymes; and also show that through the TGF-β and MAPK pathways, the synthesis and degradation of collagen in skin are regulated. These results show that deer bone collagen peptide can improve skin problems caused by aging, promote skin hydration and antioxidant capacity of aging mice, and regulate collagen synthesis and degradation through the MAPK pathway.
... It has been proven that after oral ingestion of collagen in hydrolysed form, high concentrations of small-molecule collagen peptides are recorded in the blood [20,21,22,23]. ...
... The biologically active form of the Pro-Hyp dipeptide initiates fibroblast migration and the growth of these cells in the skin. This action has a positive effect on the skin's structure, as the fibroblasts stimulate the synthesis of collagen and elastic fibers, resulting in a reduction in the signs of skin aging and an increase in skin elasticity [22,23]. Pro-Hyp dipeptide in the skin has also been shown to promote hyaluronic acid (HA) production. ...
... Kim et al. [22] investigated how oral collagen supplementation affects human skin. 64 healthy women aged 40-60 years who had dermatologists-diagnosed wrinkles participated in this study. ...
IntroductionCollagen is a protein that has found widespread use both as an ingredient in cosmetics applied externally and as an oral supplement. This substance is obtained from a number of animal sources, including bovine tissues, as well as marine fish. The characteristic properties of collagen peptides, such as scientifically proven anti-inflammatory effects, activation of fibroblast skin cells, stimulation of synovial cells to produce hyaluronic acid, and much more make this type of supplement promising in improving the body's general condition and in alleviating the symptoms of many ailments. Numerous scientific studies have shown that supplementation with hydrolyzed collagen has a beneficial effect on both the overall condition of the skin by improving its elasticity, hydration level, and positive action on its protective barrier and on the musculoskeletal system, where, when combined with appropriately selected physical activity, it can help to reduce pain associated with conditions such as osteoarthritis and even increase the range of movement in the joints. Easy availability, user-friendly forms of administration, such as powders or ready-to-drink liquid formulations, and a low incidence of side effects make this supplementation highly beneficial for users. Aim of the studyThis study aims to discuss the known processes and review the evidence supporting the beneficial effects of hydrolyzed collagen supplementation on the human skin and musculoskeletal system.Material and methodThis article presents the current state of knowledge about the benefits of oral collagen peptide supplementation in various scientific articles. Publications describing the mechanisms of action and the effects of collagen supplementation on skin conditions and the musculoskeletal system, including recent reports in this field, were reviewed using the PubMed platform. The search included the keywords ‘collagen’, ‘collagen supplementation’, ‘nutritional supplement’, ‘recovery’
... Numerous studies have reported that the supplementation of collagen or its tripeptides improves skin wrinkles, elasticity, and hydration [13][14][15]. Collagen tripeptides can be a potent anti-aging component, enhancing the synthesis of type 1 collagen and suppressing matrix metalloproteinase (MMP) protein expression [5,16]. A recent study reported hydrolyzed marine collagen ameliorates skin health in an aging population by improving skin wrinkles, elasticity, hydration, radiance, and firmness [17]. ...
... The average molecular weight of the entire Tripep20 collagen was 500 Da, with a total content of 20% tripeptides containing 3.2% Gly-Pro-Hyp. Other in vivo studies confirmed the stability and bioavailability of Gly-Pro-Hyp [11,14]. A 50 mL bottle contained 1,000 mg of Tripep20 and vehicle materials ( Table 1). ...
... Based on the guidelines for estimating the maximum safe dose in initial clinical trials of healthy subjects, 1,000 mg of Tripep20 appeared suitable for humans with an average body weight of 60 kg [18]. In addition, several comparable studies using doses from 1.0 to 3.0 g/ day have been reported [14,[19][20][21]. ...
Background/objectives:
Collagen is commonly used in diverse forms as a functional component in skincare products. On the other hand, the effects of collagen on human skin are controversial. Dietary collagen hydrolysates from freshwater Pangasius hypophthalmus fish skin ameliorated photo-aged skin of hairless mice. This study conducted a randomized, double-blind, placebo-controlled clinical trial to determine if liquid fish collagen (Collagen-Tripep20™, Tripep20) as a drink strengthens skin health and quality.
Subjects/methods:
In this clinical trial, 85 subjects aged 35-60 yrs were diagnosed with photo-aged skin. Eighty-five subjects were randomized to receive either Tripep20 (n = 44) or placebo (n = 41). Seventy-eight subjects fully participating for a 12-week period consumed 1,000 mg of Tripep20 (n = 41) or placebo (n = 37) in a 50-mL bottle as a daily drink. The intend-to-treat and per-protocol populations were 85 and 78, respectively. Skin hydration, wrinkles, and elasticity were assessed at 0 (baseline), 6, and 12 weeks during the study period.
Results:
Skin hydration in the Tripep20 group was significantly higher from 6 weeks (P < 0.001) than the baseline. After 12 weeks, the Crow's-feet visual score and skin roughness (Ra, Rq, and Rmax) were significantly improved in the Tripep20 group than in the placebo group (P < 0.05). Consuming liquid collagen Tripep20 greatly enhanced skin elasticity (Gross R2, Net R5, and Biological elasticity R7) in 6 weeks compared to the placebo group. The Tripep20 group showed a significant increase in skin elasticity from the baseline after 6 and 12 weeks (P < 0.001). Neither abnormal symptoms nor adverse events were encountered during the study period in subjects ingesting Tripep20 or placebo. The changes in parameters related to hematology and clinical chemistry were within the normal ranges.
Conclusion:
Oral consumption of liquid collagen Tripep20 was safe and well-tolerated. The results of this study show that freshwater fish-derived liquid collagen Tripep20 can be used as a healthy functional food ingredient to improve skin moisturizing, anti-wrinkling, and elasticity in an aging population.
... Low molecular weight collagen peptide improves skin hydration and skin elasticity without any side-effect [55]. Collagen improves UV-B induced skin damage because of its antioxidant properties [56]. ...
Collagen is an excellent biomaterial. Biological characteristics of collagen including biodegradability and weak antigenicity made it a primary resource in medical application. In biomedical field collagen is mainly used as sponges for wound/burn, supplement in rheumatoid arthritis, drug delivery system, controlling material for transdermal delivery and basic matrices for cell culture system due to its ability to stimulate formation of tissue and organ. Moreover, collagen is also applied in tissue engineering including bone defect, tissue regeneration, skin replacement and artificial blood vessels and valves. The article reviews biomedical application of collagen in wound healing, rheumatoid arthritis, drug delivery system, tissue engineering and lung function improvement in pulmonary fibrosis due to viral infection.
... Collagen is often used as a regenerative biomaterial due to its high biodegradability, solubility, and tensile strength [4][5][6][7][8][9]. Its low immunogenicity and excellent biocompatibility have prompted extensive research into its application as a polymer across various biomedical products, including cosmetics and pharmaceuticals [5]. ...
... While the aging process of the skin is considered complex, the incorporation of marine collagen in anti-aging supplements has been used to treat select skin concerns, including visible signs of aging [1]. In particular, the application of marine collagen peptides (MCPs) emerges as a promising therapeutic according to multiple animal and in vitro studies [7,11,[16][17][18]. MCPs are obtained by hydrolyzing collagen into small peptides of low molecular weight to improve bioavailability and absorption [19]. ...
... Among the most popular products is marine-derived collagen used for skin health and restoration. Several papers have cited the effects of collagen supplementation on skin appearance [7,8,[20][21][22]32]. ...
Aging is closely associated with collagen degradation, impacting the structure and strength of the muscles, joints, bones, and skin. The continuous aging of the skin is a natural process that is influenced by extrinsic factors such as UV exposure, dietary patterns, smoking habits, and cosmetic supplements. Supplements that contain collagen can act as remedies that help restore vitality and youth to the skin, helping combat aging. Notably, collagen supplements enriched with essential amino acids such as proline and glycine, along with marine fish collagen, have become popular for their safety and effectiveness in mitigating the aging process. To compile the relevant literature on the anti-aging applications of marine collagen, a search and analysis of peer-reviewed papers was conducted using PubMed, Cochrane Library, Web of Science, and Embase, covering publications from 1991 to 2024. From in vitro to in vivo experiments, the reviewed studies elucidate the anti-aging benefits of marine collagen, emphasizing its role in combating skin aging by minimizing oxidative stress, photodamage, and the appearance of wrinkles. Various bioactive marine peptides exhibit diverse anti-aging properties, including free radical scavenging, apoptosis inhibition, lifespan extension in various organisms, and protective effects in aging humans. Furthermore, the topical application of hyaluronic acid is discussed as a mechanism to increase collagen production and skin moisture, contributing to the anti-aging effects of collagen supplementation. The integration of bio-tissue engineering in marine collagen applications is also explored, highlighting its proven utility in skin healing and bone regeneration applications. However, limitations to the scope of its application exist. Thus, by delving into these nuanced considerations, this review contributes to a comprehensive understanding of the potential and challenges associated with marine collagen in the realm of anti-aging applications.
... 31 Another study by Kim DU et al. documented dropout rates of 17% overall, with 21% in the test group. 32 Additionally, a previous report in our group observed an 18% dropout rate overall, with 11% in the test group. 33 This study had a few limitations. ...
Background
Recent in vitro and in vivo studies have suggested that the elastin peptide improves the skin's biophysical properties, enhancing the proliferation of fibroblasts and elastin synthesis, resulting in anti‐aging properties. Therefore, we conducted a randomized, double‐blinded, placebo‐controlled study to clinically evaluate the effect of elastin peptide intake on human skin.
Materials and Methods
Healthy adult participants (N = 100) were randomly assigned to receive a test product containing 100 mg of Bonito elastin peptide (VGPG Elastin®) or placebo. In this study, all participants were Asian from Korea. The parameters of skin wrinkles, hydration, and brightening (melanin index) were measured at baseline and 4, 8, and 12 weeks after intervention.
Results
The average skin roughness, maximum peak‐to‐valley values, maximum peak height of the wrinkle, maximum valley depth of the wrinkle, average maximum height of the wrinkle, and eye wrinkle volume improved considerably in the test group compared with the placebo after 12 weeks of intervention. Skin hydration was enhanced, and the melanin index was significantly lower in the test group than in the placebo group. No participant experienced adverse events related to the test product.
Conclusion
Oral consumption of Bonito elastin peptide (VGPG Elastin®) reduced fine wrinkles, enhanced skin moisture, and decreased melanin index without significant adverse effects and may be a promising anti‐wrinkle, anti‐dryness, and anti‐pigmentation treatment.
... The corneometer is widely employed to appraise topical products' effectiveness and gauge the skin's general condition by offering valuable insights into the skin's moisture barrier. Consequently, it is helpful for measuring skin hydration levels and appraising the efficacy of skincare products [2,7,10,12]. ...
... Cutometry finds extensive use in research and clinical contexts to evaluate skin elasticity levels and track the evolution of the skin's condition over time. Generally, it is a dependable and secure tool for skin health assessment [2,3,7,12,14,16,17]. ...
... Our meta-analysis of 14 studies on oral administration of HC after four weeks proved it improves skin hydration [2,9,[11][12][13][14][15]19], elasticity [2,5,[8][9][10][12][13][14]16,17], wrinkles [2,9,10,12,[17][18][19], TEWL [10,17,19], firmness [2,9,19], and radiance [9,11]. ...
With increasing life expectancy, the quest for skin rejuvenation has gained prominence among individuals of diverse age groups. The popularity of nutricosmetics, notably dietary supplements, has garnered significant attention in recent years. Many scientific investigations have amassed compelling evidence highlighting the positive impact of hydrolyzed collagen supplementation in mitigating the visible signs of skin aging. This study aims to know the powerful effect of hydrolyzed collagen on the skin. This research method is to conduct a systematic review followed by a meta-analysis of the clinical trial focusing on randomized, double-blind, and controlled trials that examined the oral consumption of hydrolyzed collagen and reported outcomes related to skin aging, wrinkles, moisture levels, elasticity, and firmness. The selected articles from CENTRAL, PubMed, Google Scholar, and ScienceDirect databases were published from 2017 to 2023. The subsequent meta-analysis, comprising 14 distinct studies and a collective cohort of 967 participants, revealed encouraging findings favoring hydrolyzed collagen supplementation. It consistently demonstrated substantial enhancements in skin moisture levels and elasticity compared to the placebo group, a trend robustly corroborated by subgroup analysis. These compelling findings underscore the effectiveness of a 12-week regimen of hydrolyzed collagen supplementation in revitalizing the skin by augmenting its hydration and elasticity.
... LMCP is individually recognized as a health functional food ingredient for skin function by the Ministry of Food and Drug Safety in the Republic of Korea. In our previous study, oral LMCP administration displayed health benefits in human skin by reducing wrinkles and increasing hydration and elasticity [16]. Through a mechanism study, we confirmed that LMCP stimulated skin fibroblasts to synthesize type I collagen and hyaluronic acid [17,18]. ...
This study reveals that low-molecular-weight collagen peptide (LMWCP) can stimulate the differentiation and the mineralization of MC3T3-E1 cells in vitro and attenuate the bone remodeling process in ovariectomized (OVX) Sprague-Dawley rats in vivo. Moreover, the assessed LMWCP increased the activity of alkaline phosphatase (ALP), synthesis of collagen, and mineralization in MC3T3-E1 cells. Additionally, mRNA levels of bone metabolism-related factors such as the collagen type I alpha 1 chain, osteocalcin (OCN), osterix, bone sialoprotein, and the Runt family-associated transcription factor 2 were increased in cells treated with 1,000 μg/ml of LMWCP. Furthermore, we demonstrated that critical bone morphometric parameters exhibited significant differences between the LMWCP (400 mg/kg)-receiving and vehicle-treated rat groups. Moreover, the expression of type I collagen and the activity of ALP were found to be higher in both the femur and lumbar vertebrae of OVX rats treated with LMWCP. Finally, the administration of LMWCP managed to alleviate osteogenic parameters such as the ALP activity and the levels of the bone alkaline phosphatase, the OCN, and the procollagen type 1 N-terminal propeptide in OVX rats. Thus, our findings suggest that LMWCP is a promising candidate for the development of food-based prevention strategies against osteoporosis.
... Collagen and elastin are two primary structural constituents of the dermal extracellular matrix that provide the skin with resistance to pressure and stretching. 1 The aging process, including ultraviolet radiation, mechanical stress, and the intrinsic process of senescence, induces shorter and less organized collagen fibers of the dermal collagen network that decrease the collagen density of the dermis and dermal thickness. 2 These intrinsic and extrinsic aging processes make the skin lose elasticity and become vulnerable to damage. ...
Collagen peptides enriched with X-Hyp or X-Hyp-Gly have demonstrated resistance to digestive and systemic enzymes, suggesting their potential for improved absorption efficiency and enhancement of skin properties. This study aimed...
